LOCUS       sequence-391210-        3519 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick (MoClo) Level 0 Part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 3519)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pMC-6-NnH3H-9
FEATURES             Location/Qualifiers
     source          1..3519
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1399..1417)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1485..1589
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1811..2602
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2773..3361
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3262..3281
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(3515..3519,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcaa atggcatcct ttgaaaattc actcagtgtc
      121 ttaatagttg gggctggctt aggtggtttg gccgctgcta tagcactgcg aaggcaagga
      181 catgtagtca agatatacga tagttcttca ttcaaggccg aattgggagc aggattagct
      241 gtaccaccca atactctccg ttccctgcaa caactgggct gtaatacaga aaacctgaac
      301 ggagtagaca acctctgctt tacggccatg ggatacgacg ggagtgtagg tatgatgaat
      361 aatatgaccg actaccgtga ggcatacggc acttcctgga taatggtaca tcgagtggat
      421 ctccacaacg agctgatgcg agtcgccttg gatccgggcg ggttagggcc tcctgccact
      481 cttcacctta accatcgtgt tacgttctgc gatgtggatg cctgtacagt cacgtttacg
      541 aacgggacga cacagtctgc tgacttgatt gtcggagctg atggtattag gtctacgatt
      601 cgaagattcg ttctggagga ggacgtgact gttccagcta gcggaattgt cggctttcga
      661 tggttggtcc aggccgatgc tctcgaccca tatcccgaac tggattggat tgtcaaaaaa
      721 ccacccttgg gagccaggtt gatttctact ccacagaacc cgcagagtgg cgtaggactg
      781 gccgatagac gaacgatcat tatttacgca tgcagaggtg ggaccatggt taatgtatta
      841 gccgtacacg acgacgaaag agatcaaaac actgctgatt ggtccgttcc cgcatcaaag
      901 gatgatttat tcagagtgtt tcacgattac catccaagat tccgacgact cttagagtta
      961 gcacaggaca ttaatttatg gcagatgagg gtggtcccgg tcttgaaaaa atgggtgaac
     1021 aagagggtat gcttgctggg tgatgccgcc catgcttcac tgcccacact tggacaaggc
     1081 ttcgggatgg ggttggagga tgcagtcgct ttgggcacgt tgttgcctaa gggaacgacc
     1141 gcatcacaga tcgagacaag gctggccgtt tacgaacagc tgcgaaagga tcgagctgag
     1201 tttgtcgccg cagagagcta tgaggagcaa tatgtcccag agatgagagg gctgtacctt
     1261 aggtccaaag aattgagaga cagagtgatg ggttatgata ttaaggtaga gtcagagaag
     1321 gttttggaaa cactgttgcg atcatccaac tcagcttgag ctttgagacc acgaagtggc
     1381 tcttcagtgg acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     1441 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     1501 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     1561 tgcttcaata atattgaaaa aggaagagta tgcgctcacg caactggtcc agaaccttga
     1621 ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt
     1681 ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg
     1741 atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa
     1801 gttaaacatc atgagggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt
     1861 tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc
     1921 agtggatggc ggcctgaagc cacacagcga tattgatttg ctggttacgg tgaccgtaag
     1981 gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc
     2041 tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat
     2101 tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat
     2161 tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa
     2221 agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt
     2281 tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc
     2341 cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc
     2401 agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc
     2461 ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga
     2521 tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cattacgtaa aaggcgagat
     2581 caccaaggta gtcggcaaat aactgtcaga ccaagtttac tcatatatac tttagattga
     2641 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     2701 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     2761 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     2821 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     2881 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     2941 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     3001 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     3061 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     3121 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     3181 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     3241 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     3301 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     3361 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     3421 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     3481 tgataccgct cgccgcagcc gaacgaccga gcgcagcga
//
