LOCUS       sequence-391213-       10671 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A pVS1-based T-DNA binary vector with NPTII as a plant selectable 
            marker gene. BsaI site was removed..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10671)
  AUTHORS   Lee K, Kang M, Ji Q, Grosic S, Wang K
  TITLE     New T-DNA binary vectors with NptII selection and RUBY reporter for 
            efficient maize transformation and targeted mutagenesis.
  JOURNAL   Plant Physiol. 2023 Apr 18:kiad231. doi: 10.1093/plphys/kiad231.
  PUBMED    37070560
REFERENCE   2  (bases 1 to 10671)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2023 Apr 18:kiad231. doi: 10.1093/plphys/kiad231."
COMMENT     Sequence Label: pCBL101.1
FEATURES             Location/Qualifiers
     source          1..10671
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    183..204
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(219..224,225..242,243..249)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    257..273
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     262..284
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     281..297
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     281..297
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        427..2422
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     protein_bind    2451..2498
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             2518..3312
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MVEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2572..2591)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3182..3201
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(3879..3896)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3879..3895)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3888..3910)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    4110..4134
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             4655..5446
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5692..6280
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6181..6200
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6434..6451
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6466..6606
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6532..6551)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6692..6714
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      6950..7144
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7210..8283)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(8712..9341)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
       61 aatctgatcc aagctcaagc tccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
      121 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
      181 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
      241 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgacatg
      301 attacgaatt cgagctctat tgagaccggc gcgcctaggt accacgcgtc cgcgggtctc
      361 accaggggat cctctagagt cgacctgcag gcatgcaata gttccggact taccggcgcc
      421 gctagcctgc agtgcagcgt gacccggtcg tgcccctctc tagagataat gagcattgca
      481 tgtctaagtt ataaaaaatt accacatatt ttttttgtca cacttgtttg aagtgcagtt
      541 tatctatctt tatacatata tttaaacttt actctacgaa taatataatc tatagtacta
      601 caataatatc agtgttttag agaatcatat aaatgaacag ttagacatgg tctaaaggac
      661 aattgagtat tttgacaaca ggactctaca gttttatctt tttagtgtgc atgtgttctc
      721 cttttttttt gcaaatagct tcacctatat aatacttcat ccattttatt agtacatcca
      781 tttagggttt agggttaatg gtttttatag actaattttt ttagtacatc tattttattc
      841 tattttagcc tctaaattaa gaaaactaaa actctatttt agttttttta tttaataatt
      901 tagatataaa atagaataaa ataaagtgac taaaaattaa acaaataccc tttaagaaat
      961 taaaaaaact aaggaaacat ttttcttgtt tcgagtagat aatgccagcc tgttaaacgc
     1021 cgtcgacgag tctaacggac accaaccagc gaaccagcag cgtcgcgtcg ggccaagcga
     1081 agcagacggc acggcatctc tgtcgctgcc tctggacccc tctcgagagt tccgctccac
     1141 cgttggactt gctccgctgt cggcatccag aaattgcgtg gcggagcggc agacgtgagc
     1201 cggcacggca ggcggcctcc tcctcctctc acggcaccgg cagctacggg ggattccttt
     1261 cccaccgctc cttcgctttc ccttcctcgc ccgccgtaat aaatagacac cccctccaca
     1321 ccctctttcc ccaacctcgt gttgttcgga gcgcacacac acacaaccag atctccccca
     1381 aatccacccg tcggcacctc cgcttcaagg tacgccgctc gtcctccccc ccccccctct
     1441 ctaccttctc tagatcggcg ttccggtcca tgcatggtta gggcccggta gttctacttc
     1501 tgttcatgtt tgtgttagat ccgtgtttgt gttagatccg tgctgctagc gttcgtacac
     1561 ggatgcgacc tgtacgtcag acacgttctg attgctaact tgccagtgtt tctctttggg
     1621 gaatcctggg atggctctag ccgttccgca gacgggatcg atttcatgat tttttttgtt
     1681 tcgttgcata gggtttggtt tgcccttttc ctttatttca atatatgccg tgcacttgtt
     1741 tgtcgggtca tcttttcatg cttttttttg tcttggttgt gatgatgtgg tctggttggg
     1801 cggtcgttct agatcggagt agaattctgt ttcaaactac ctggtggatt tattaatttt
     1861 ggatctgtat gtgtgtgcca tacatattca tagttacgaa ttgaagatga tggatggaaa
     1921 tatcgatcta ggataggtat acatgttgat gcgggtttta ctgatgcata tacagagatg
     1981 ctttttgttc gcttggttgt gatgatgtgg tgtggttggg cggtcgttca ttcgttctag
     2041 atcggagtag aatactgttt caaactacct ggtgtattta ttaattttgg aactgtatgt
     2101 gtgtgtcata catcttcata gttacgagtt taagatggat ggaaatatcg atctaggata
     2161 ggtatacatg ttgatgtggg ttttactgat gcatatacat gatggcatat gcagcatcta
     2221 ttcatatgct ctaaccttga gtacctatct attataataa acaagtatgt tttataatta
     2281 ttttgatctt gatatacttg gatgatggca tatgcagcag ctatatgtgg atttttttag
     2341 ccctgccttc atacgctatt tatttgcttg gtactgtttc ttttgtcgat gctcaccctg
     2401 ttgtttggtg ttacttctgc aggtcgactc tagaggatca attcgctagc gaagttccta
     2461 ttccgaagtt cctattctct agaaagtata ggaacttcag atccaccggg atccaccatg
     2521 gttgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
     2581 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
     2641 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag
     2701 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
     2761 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
     2821 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
     2881 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
     2941 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
     3001 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc
     3061 gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
     3121 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
     3181 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
     3241 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
     3301 gagttcttct gaggatccac catggttaac ctagacttgt ccatcttctg gattggccaa
     3361 cttaattaat gtatgaaata aaaggatgca cacatagtga catgctaatc actataatgt
     3421 gggcatcaaa gttgtgtgtt atgtgtaatt actagttatc tgaataaaag agaaagagat
     3481 catccatatt tcttatccta aatgaatgtc acgtgtcttt ataattcttt gatgaaccag
     3541 atgcatttca ttaaccaaat ccatatacat ataaatatta atcatatata attaatatca
     3601 attgggttag caaaacaaat ctagtctagg tgtgttttgc aagcttgcat gcctgcagtc
     3661 gtctattgga ccgacagttg ctattaatat aatgggccac catagtagac tgacaaataa
     3721 attacctgac aacatcgttt cacaaaaaaa caaacacaaa aagggagtgc attttccagg
     3781 gcatttttgt aataaaaaac agttaaaagg gagtgcaata gaaatatagg ggtgtggaaa
     3841 tagtgatttg agcacgtctt gaagcgaatt agcttggcac tggccgtcgt tttacaacgt
     3901 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     3961 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     4021 ctgaatggcg aatgctagag caattcggcg ttaattcagt acattaaaaa cgtccgcaat
     4081 gtgttattaa gttgtctaag cgtcaatttg tttacaccac aatatatcct gccaccagcc
     4141 agccaacagc tccccgaccg gcagctcggc acaaaatcac cactcgatac aggcagccca
     4201 tcagtccggg acggcgtcag cgggagagcc gttgtaaggc ggcagacttt gctcatgtta
     4261 ccgatgctat tcggaagaac ggcaactaag ctgccgggtt tgaaacacgg atgatctcgc
     4321 ggagggtagc atgttgattg taacgatgac agagcgttgc tgcctgtgat caattcgggc
     4381 acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg
     4441 ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt
     4501 tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg catccaagca
     4561 gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg atgttacgca
     4621 gcagggcagt cgccctaaaa caaagttaaa catcatgggg gaagcggtga tcgccgaagt
     4681 atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct
     4741 ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca gtgatattga
     4801 tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga
     4861 ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg tagaagtcac
     4921 cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt
     4981 tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca caatcgacat
     5041 tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc
     5101 ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac
     5161 cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac
     5221 gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc
     5281 cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg aagctagaca
     5341 ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt
     5401 tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataatgtc tagctagaaa
     5461 ttcgttcaag ccgacgccgc ttcgcggcgc ggcttaactc aagcgttaga tgcactaagc
     5521 acataattgc tcacagccaa actatcaggt caagtctgct tttattattt ttaagcgtgc
     5581 ataataagcc ctacacaaat tgggagatat atcatgcatg accaaaatcc cttaacgtga
     5641 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     5701 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     5761 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     5821 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     5881 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     5941 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     6001 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     6061 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     6121 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     6181 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     6241 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     6301 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     6361 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     6421 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt
     6481 tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg
     6541 ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg
     6601 gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
     6661 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
     6721 ccgtcatcac cgaaacgcgc gaggcagggt gccttgatgt gggcgccggc ggtcgagtgg
     6781 cgacggcgcg gcttgtccgc gccctggtag attgcctggc cgtaggccag ccatttttga
     6841 gcggccagcg gccgcgatag gccgacgcga agcggcgggg cgtagggagc gcagcgaccg
     6901 aagggtaggc gctttttgca gctcttcggc tgtgcgctgg ccagacagtt atgcacaggc
     6961 caggcgggtt ttaagagttt taataagttt taaagagttt taggcggaaa aatcgccttt
     7021 tttctctttt atatcagtca cttacatgtg tgaccggttc ccaatgtacg gctttgggtt
     7081 cccaatgtac gggttccggt tcccaatgta cggctttggg ttcccaatgt acgtgctatc
     7141 cacaggaaag ataccttttc gacctttttc ccctgctagg gcaatttgcc ctagcatctg
     7201 ctccgtacat taggaaccgg cggatgcttc gccctcgatc aggttgcggt agcgcatgac
     7261 taggatcggg ccagcctgcc ccgcctcctc cttcaaatcg tactccggca ggtcatttga
     7321 cccgatcagc ttgcgcacgg tgaaacagaa cttcttgaac tctccggcgc tgccactgcg
     7381 ttcgtagatc gtcttgaaca accatctggc ttctgccttg cctgcggcgc ggcgtgccag
     7441 gcggtagaga aaacggccga tgccgggatc gatcaaaaag taatcggggt gaaccgtcag
     7501 cacgtccggg ttcttgcctt ctgtgatctc gcggtacatc caatcagcta gctcgatctc
     7561 gatgtactcc ggccgcccgg tttcgctctt tacgatcttg tagcggctaa tcaaggcttc
     7621 accctcggat accgtcacca ggcggccgtt cttggccttc ttcgtacgct gcatggcaac
     7681 gtgcgtggtg tttaaccgaa tgcaggtttc taccaggtcg tctttctgct ttccgccatc
     7741 ggctcgccgg cagaacttga gtacgtccgc aacgtgtgga cggaacacgc ggccgggctt
     7801 gtctcccttc ccttcccggt atcggttcat ggattcggtt agatgggaaa ccgccatcag
     7861 taccaggtcg taatcccaca cactggccat gccggccggc cctgcggaaa cctctacgtg
     7921 cccgtctgga agctcgtagc ggatcacctc gccagctcgt cggtcacgct tcgacagacg
     7981 gaaaacggcc acgtccatga tgctgcgact atcgcgggtg cccacgtcat agagcatcgg
     8041 aacgaaaaaa tctggttgct cgtcgccctt gggcggcttc ctaatcgacg gcgcaccggc
     8101 tgccggcggt tgccgggatt ctttgcggat tcgatcagcg gccgcttgcc acgattcacc
     8161 ggggcgtgct tctgcctcga tgcgttgccg ctgggcggcc tgcgcggcct tcaacttctc
     8221 caccaggtca tcacccagcg ccgcgccgat ttgtaccggg ccggatggtt tgcgaccgct
     8281 cacgccgatt cctcgggctt gggggttcca gtgccattgc agggccggca gacaacccag
     8341 ccgcttacgc ctggccaacc gcccgttcct ccacacatgg ggcattccac ggcgtcggtg
     8401 cctggttgtt cttgattttc catgccgcct cctttagccg ctaaaattca tctactcatt
     8461 tattcatttg ctcatttact ctggtagctg cgcgatgtat tcagatagca gctcggtaat
     8521 ggtcttgcct tggcgtaccg cgtacatctt cagcttggtg tgatcctccg ccggcaactg
     8581 aaagttgacc cgcttcatgg ctggcgtgtc tgccaggctg gccaacgttg cagccttgct
     8641 gctgcgtgcg ctcggacggc cggcacttag cgtgtttgtg cttttgctca ttttctcttt
     8701 acctcattaa ctcaaatgag ttttgattta atttcagcgg ccagcgcctg gacctcgcgg
     8761 gcagcgtcgc cctcgggttc tgattcaaga acggttgtgc cggcggcggc agtgcctggg
     8821 tagctcacgc gctgcgtgat acgggactca agaatgggca gctcgtaccc ggccagcgcc
     8881 tcggcaacct caccgccgat gcgcgtgcct ttgatcgccc gcgacacgac aaaggccgct
     8941 tgtagccttc catccgtgac ctcaatgcgc tgcttaacca gctccaccag gtcggcggtg
     9001 gcccatatgt cgtaagggct tggctgcacc ggaatcagca cgaagtcggc tgccttgatc
     9061 gcggacacag ccaagtccgc cgcctggggc gctccgtcga tcactacgaa gtcgcgccgg
     9121 ccgatggcct tcacgtcgcg gtcaatcgtc gggcggtcga tgccgacaac ggttagcggt
     9181 tgatcttccc gcacggccgc ccaatcgcgg gcactgccct ggggatcgga atcgactaac
     9241 agaacatcgg ccccggcgag ttgcagggcg cgggctagat gggttgcgat ggtcgtcttg
     9301 cctgacccgc ctttctggtt aagtacagcg ataaccttca tgcgttcccc ttgcgtattt
     9361 gtttatttac tcatcgcatc atatacgcag cgaccgcatg acgcaagctg ttttactcaa
     9421 atacacatca cctttttaga cggcggcgct cggtttcttc agcggccaag ctggccggcc
     9481 aggccgccag cttggcatca gacaaaccgg ccaggatttc atgcagccgc acggttgaga
     9541 cgtgcgcggg cggctcgaac acgtacccgg ccgcgatcat ctccgcctcg atctcttcgg
     9601 taatgaaaaa cggttcgtcc tggccgtcct ggtgcggttt catgcttgtt cctcttggcg
     9661 ttcattctcg gcggccgcca gggcgtcggc ctcggtcaat gcgtcctcac ggaaggcacc
     9721 gcgccgcctg gcctcggtgg gcgtcacttc ctcgctgcgc tcaagtgcgc ggtacagggt
     9781 cgagcgatgc acgccaagca gtgcagccgc ctctttcacg gtgcggcctt cctggtcgat
     9841 cagctcgcgg gcgtgcgcga tctgtgccgg ggtgagggta gggcgggggc caaacttcac
     9901 gcctcgggcc ttggcggcct cgcgcccgct ccgggtgcgg tcgatgatta gggaacgctc
     9961 gaactcggca atgccggcga acacggtcaa caccatgcgg ccggccggcg tggtggtgtc
    10021 ggcccacggc tctgccaggc tacgcaggcc cgcgccggcc tcctggatgc gctcggcaat
    10081 gtccagtagg tcgcgggtgc tgcgggccag gcggtctagc ctggtcactg tcacaacgtc
    10141 gccagggcgt aggtggtcaa gcatcctggc cagctccggg cggtcgcgcc tggtgccggt
    10201 gatcttctcg gaaaacagct tggtgcagcc ggccgcgtgc agttcggccc gttggttggt
    10261 caagtcctgg tcgtcggtgc tgacgcgggc atagcccagc aggccagcgg cggcgctctt
    10321 gttcatggcg taatgtctcc ggttctagtc gcaagtattc tactttatgc gactaaaaca
    10381 cgcgacaaga aaacgccagg aaaagggcag ggcggcagcc tgtcgcgtaa cttaggactt
    10441 gtgcgacatg tcgttttcag aagacggctg cactgaacgt cagaagccga ctgcactata
    10501 gcagcggagg ggttggatca aagtacttta aagtacttta aagtacttta aagtactttg
    10561 atcccgaggg gaaccctgtg gttggcatgc acatacaaat ggacgaacgg ataaaccttt
    10621 tcacgccctt ttaaatatcc gattattcta ataaacgctc ttttctctta g
//
