LOCUS       sequence-391217-       12670 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Golden Gate recipient vector for transgene overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12670)
  AUTHORS   Lee K, Kang M, Ji Q, Grosic S, Wang K
  TITLE     New T-DNA binary vectors with NptII selection and RUBY reporter for 
            efficient maize transformation and targeted mutagenesis.
  JOURNAL   Plant Physiol. 2023 Apr 18:kiad231. doi: 10.1093/plphys/kiad231.
  PUBMED    37070560
REFERENCE   2  (bases 1 to 12670)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2023 Apr 18:kiad231. doi: 10.1093/plphys/kiad231."
COMMENT     Sequence Label: pCBL101gg
FEATURES             Location/Qualifiers
     source          1..12670
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    183..204
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(219..224,225..242,243..249)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    257..273
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     262..284
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     281..297
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     281..297
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        367..812
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             join(813..899,900..2234)
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVSEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     promoter        2427..4421
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     protein_bind    4450..4497
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             4517..5311
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MVEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4571..4590)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5181..5200
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5878..5895)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5878..5894)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5887..5909)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    6109..6133
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6654..7445
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7691..8279
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8180..8199
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8433..8450
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8465..8605
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8531..8550)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8691..8713
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8949..9143
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9209..10282)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10711..11340)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
       61 aatctgatcc aagctcaagc tccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
      121 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
      181 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
      241 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgacatg
      301 attacgaatt cgagctctat tgagaccggc tggggttcgc tagaggatcg atccttttta
      361 acccatcaca tatacctgcc gttcactatt atttagtgaa atgagatatt atgatatttt
      421 ctgaattgtg attaaaaagg caactttatg cccatgcaac agaaactata aaaaatacag
      481 agaatgaaaa gaaacagata gattttttag ttctttaggc ccgtagtctg caaatccttt
      541 tatgattttc tatcaaacaa aagaggaaaa tagaccagtt gcaatccaaa cgagagtcta
      601 atagaatgag gtcgaaaagt aaatcgcgcg ggtttgttac tgataaagca ggcaagacct
      661 aaaatgtgta aagggcaaag tgtatacttt ggcgtcaccc cttacatatt ttaggtcttt
      721 ttttattgtg cgtaactaac ttgccatctt caaacaggag ggctggaaga agcagaccgc
      781 taacacagta cataaaaaag gagacatgaa cgatgaacat caaaaagttt gcaaaacaag
      841 caacagtatt aacctttact accgcactgc tggcaggagg cgcaactcaa gcgtttgcga
      901 aagaaacgaa ccaaaagcca tataaggaaa catacggcat ttcccatatt acacgccatg
      961 atatgctgca aatccctgaa cagcaaaaaa atgaaaaata tcaagtttct gaatttgatt
     1021 cgtccacaat taaaaatatc tcttctgcaa aaggcctgga cgtttgggac agctggccat
     1081 tacaaaacgc tgacggcact gtcgcaaact atcacggcta ccacatcgtc tttgcattag
     1141 ccggagatcc taaaaatgcg gatgacacat cgatttacat gttctatcaa aaagtcggcg
     1201 aaacttctat tgacagctgg aaaaacgctg gccgcgtctt taaagacagc gacaaattcg
     1261 atgcaaatga ttctatccta aaagaccaaa cacaagaatg gtcaggttca gccacattta
     1321 catctgacgg aaaaatccgt ttattctaca ctgatttctc cggtaaacat tacggcaaac
     1381 aaacactgac aactgcacaa gttaacgtat cagcatcaga cagctctttg aacatcaacg
     1441 gtgtagagga ttataaatca atctttgacg gtgacggaaa aacgtatcaa aatgtacagc
     1501 agttcatcga tgaaggcaac tacagctcag gcgacaacca tacgctgaga gatcctcact
     1561 acgtagaaga taaaggccac aaatacttag tatttgaagc aaacactgga actgaagatg
     1621 gctaccaagg cgaagaatct ttatttaaca aagcatacta tggcaaaagc acatcattct
     1681 tccgtcaaga aagtcaaaaa cttctgcaaa gcgataaaaa acgcacggct gagttagcaa
     1741 acggcgctct cggtatgatt gagctaaacg atgattacac actgaaaaaa gtgatgaaac
     1801 cgctgattgc atctaacaca gtaacagatg aaattgaacg cgcgaacgtc tttaaaatga
     1861 acggcaaatg gtacctgttc actgactccc gcggatcaaa aatgacgatt gacggcatta
     1921 cgtctaacga tatttacatg cttggttatg tttctaattc tttaactggc ccatacaagc
     1981 cgctgaacaa aactggcctt gtgttaaaaa tggatcttga tcctaacgat gtaaccttta
     2041 cttactcaca cttcgctgta cctcaagcga aaggaaacaa tgtcgtgatt acaagctata
     2101 tgacaaacag aggattctac gcagacaaac aatcaacgtt tgcgccgagc ttcctgctga
     2161 acatcaaagg caagaaaaca tctgttgtca aagacagcat ccttgaacaa ggacaattaa
     2221 cagttaacaa ataaaaacgc aaaagaaaat gccgatgggt accgagcgaa atgaccgacc
     2281 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt
     2341 tgggcttcgg aatgggtctc accaggggat cctctagagt cgacctgcag gcatgcaata
     2401 gttccggact taccggcgcc gctagcctgc agtgcagcgt gacccggtcg tgcccctctc
     2461 tagagataat gagcattgca tgtctaagtt ataaaaaatt accacatatt ttttttgtca
     2521 cacttgtttg aagtgcagtt tatctatctt tatacatata tttaaacttt actctacgaa
     2581 taatataatc tatagtacta caataatatc agtgttttag agaatcatat aaatgaacag
     2641 ttagacatgg tctaaaggac aattgagtat tttgacaaca ggactctaca gttttatctt
     2701 tttagtgtgc atgtgttctc cttttttttt gcaaatagct tcacctatat aatacttcat
     2761 ccattttatt agtacatcca tttagggttt agggttaatg gtttttatag actaattttt
     2821 ttagtacatc tattttattc tattttagcc tctaaattaa gaaaactaaa actctatttt
     2881 agttttttta tttaataatt tagatataaa atagaataaa ataaagtgac taaaaattaa
     2941 acaaataccc tttaagaaat taaaaaaact aaggaaacat ttttcttgtt tcgagtagat
     3001 aatgccagcc tgttaaacgc cgtcgacgag tctaacggac accaaccagc gaaccagcag
     3061 cgtcgcgtcg ggccaagcga agcagacggc acggcatctc tgtcgctgcc tctggacccc
     3121 tctcgagagt tccgctccac cgttggactt gctccgctgt cggcatccag aaattgcgtg
     3181 gcggagcggc agacgtgagc cggcacggca ggcggcctcc tcctcctctc acggcaccgg
     3241 cagctacggg ggattccttt cccaccgctc cttcgctttc ccttcctcgc ccgccgtaat
     3301 aaatagacac cccctccaca ccctctttcc ccaacctcgt gttgttcgga gcgcacacac
     3361 acacaaccag atctccccca aatccacccg tcggcacctc cgcttcaagg tacgccgctc
     3421 gtcctccccc cccccctctc taccttctct agatcggcgt tccggtccat gcatggttag
     3481 ggcccggtag ttctacttct gttcatgttt gtgttagatc cgtgtttgtg ttagatccgt
     3541 gctgctagcg ttcgtacacg gatgcgacct gtacgtcaga cacgttctga ttgctaactt
     3601 gccagtgttt ctctttgggg aatcctggga tggctctagc cgttccgcag acgggatcga
     3661 tttcatgatt ttttttgttt cgttgcatag ggtttggttt gcccttttcc tttatttcaa
     3721 tatatgccgt gcacttgttt gtcgggtcat cttttcatgc ttttttttgt cttggttgtg
     3781 atgatgtggt ctggttgggc ggtcgttcta gatcggagta gaattctgtt tcaaactacc
     3841 tggtggattt attaattttg gatctgtatg tgtgtgccat acatattcat agttacgaat
     3901 tgaagatgat ggatggaaat atcgatctag gataggtata catgttgatg cgggttttac
     3961 tgatgcatat acagagatgc tttttgttcg cttggttgtg atgatgtggt gtggttgggc
     4021 ggtcgttcat tcgttctaga tcggagtaga atactgtttc aaactacctg gtgtatttat
     4081 taattttgga actgtatgtg tgtgtcatac atcttcatag ttacgagttt aagatggatg
     4141 gaaatatcga tctaggatag gtatacatgt tgatgtgggt tttactgatg catatacatg
     4201 atggcatatg cagcatctat tcatatgctc taaccttgag tacctatcta ttataataaa
     4261 caagtatgtt ttataattat tttgatcttg atatacttgg atgatggcat atgcagcagc
     4321 tatatgtgga tttttttagc cctgccttca tacgctattt atttgcttgg tactgtttct
     4381 tttgtcgatg ctcaccctgt tgtttggtgt tacttctgca ggtcgactct agaggatcaa
     4441 ttcgctagcg aagttcctat tccgaagttc ctattctcta gaaagtatag gaacttcaga
     4501 tccaccggga tccaccatgg ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     4561 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     4621 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     4681 tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     4741 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     4801 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     4861 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     4921 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     4981 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     5041 ggcgcgcatg cccgacggcg atgatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     5101 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     5161 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     5221 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     5281 cttctatcgc cttcttgacg agttcttctg aggatccacc atggttaacc tagacttgtc
     5341 catcttctgg attggccaac ttaattaatg tatgaaataa aaggatgcac acatagtgac
     5401 atgctaatca ctataatgtg ggcatcaaag ttgtgtgtta tgtgtaatta ctagttatct
     5461 gaataaaaga gaaagagatc atccatattt cttatcctaa atgaatgtca cgtgtcttta
     5521 taattctttg atgaaccaga tgcatttcat taaccaaatc catatacata taaatattaa
     5581 tcatatataa ttaatatcaa ttgggttagc aaaacaaatc tagtctaggt gtgttttgca
     5641 agcttgcatg cctgcagtcg tctattggac cgacagttgc tattaatata atgggccacc
     5701 atagtagact gacaaataaa ttacctgaca acatcgtttc acaaaaaaac aaacacaaaa
     5761 agggagtgca ttttccaggg catttttgta ataaaaaaca gttaaaaggg agtgcaatag
     5821 aaatataggg gtgtggaaat agtgatttga gcacgtcttg aagcgaatta gcttggcact
     5881 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     5941 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     6001 ttcccaacag ttgcgcagcc tgaatggcga atgctagagc aattcggcgt taattcagta
     6061 cattaaaaac gtccgcaatg tgttattaag ttgtctaagc gtcaatttgt ttacaccaca
     6121 atatatcctg ccaccagcca gccaacagct ccccgaccgg cagctcggca caaaatcacc
     6181 actcgataca ggcagcccat cagtccggga cggcgtcagc gggagagccg ttgtaaggcg
     6241 gcagactttg ctcatgttac cgatgctatt cggaagaacg gcaactaagc tgccgggttt
     6301 gaaacacgga tgatctcgcg gagggtagca tgttgattgt aacgatgaca gagcgttgct
     6361 gcctgtgatc aattcgggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt
     6421 aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg
     6481 gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta
     6541 tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga
     6601 gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac atcatggggg
     6661 aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc
     6721 atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga
     6781 agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc
     6841 ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc
     6901 tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag
     6961 ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg
     7021 agccagccac aatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg
     7081 ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat
     7141 ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg
     7201 agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg
     7261 cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg
     7321 tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg
     7381 cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca
     7441 aataatgtct agctagaaat tcgttcaagc cgacgccgct tcgcggcgcg gcttaactca
     7501 agcgttagat gcactaagca cataattgct cacagccaaa ctatcaggtc aagtctgctt
     7561 ttattatttt taagcgtgca taataagccc tacacaaatt gggagatata tcatgcatga
     7621 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     7681 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     7741 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     7801 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     7861 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     7921 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     7981 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     8041 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     8101 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     8161 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     8221 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     8281 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     8341 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     8401 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     8461 agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt
     8521 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
     8581 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
     8641 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     8701 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg
     8761 ggcgccggcg gtcgagtggc gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc
     8821 gtaggccagc catttttgag cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc
     8881 gtagggagcg cagcgaccga agggtaggcg ctttttgcag ctcttcggct gtgcgctggc
     8941 cagacagtta tgcacaggcc aggcgggttt taagagtttt aataagtttt aaagagtttt
     9001 aggcggaaaa atcgcctttt ttctctttta tatcagtcac ttacatgtgt gaccggttcc
     9061 caatgtacgg ctttgggttc ccaatgtacg ggttccggtt cccaatgtac ggctttgggt
     9121 tcccaatgta cgtgctatcc acaggaaaga taccttttcg acctttttcc cctgctaggg
     9181 caatttgccc tagcatctgc tccgtacatt aggaaccggc ggatgcttcg ccctcgatca
     9241 ggttgcggta gcgcatgact aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt
     9301 actccggcag gtcatttgac ccgatcagct tgcgcacggt gaaacagaac ttcttgaact
     9361 ctccggcgct gccactgcgt tcgtagatcg tcttgaacaa ccatctggct tctgccttgc
     9421 ctgcggcgcg gcgtgccagg cggtagagaa aacggccgat gccgggatcg atcaaaaagt
     9481 aatcggggtg aaccgtcagc acgtccgggt tcttgccttc tgtgatctcg cggtacatcc
     9541 aatcagctag ctcgatctcg atgtactccg gccgcccggt ttcgctcttt acgatcttgt
     9601 agcggctaat caaggcttca ccctcggata ccgtcaccag gcggccgttc ttggccttct
     9661 tcgtacgctg catggcaacg tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt
     9721 ctttctgctt tccgccatcg gctcgccggc agaacttgag tacgtccgca acgtgtggac
     9781 ggaacacgcg gccgggcttg tctcccttcc cttcccggta tcggttcatg gattcggtta
     9841 gatgggaaac cgccatcagt accaggtcgt aatcccacac actggccatg ccggccggcc
     9901 ctgcggaaac ctctacgtgc ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc
     9961 ggtcacgctt cgacagacgg aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc
    10021 ccacgtcata gagcatcgga acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc
    10081 taatcgacgg cgcaccggct gccggcggtt gccgggattc tttgcggatt cgatcagcgg
    10141 ccgcttgcca cgattcaccg gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct
    10201 gcgcggcctt caacttctcc accaggtcat cacccagcgc cgcgccgatt tgtaccgggc
    10261 cggatggttt gcgaccgctc acgccgattc ctcgggcttg ggggttccag tgccattgca
    10321 gggccggcag acaacccagc cgcttacgcc tggccaaccg cccgttcctc cacacatggg
    10381 gcattccacg gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc ctttagccgc
    10441 taaaattcat ctactcattt attcatttgc tcatttactc tggtagctgc gcgatgtatt
    10501 cagatagcag ctcggtaatg gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt
    10561 gatcctccgc cggcaactga aagttgaccc gcttcatggc tggcgtgtct gccaggctgg
    10621 ccaacgttgc agccttgctg ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc
    10681 ttttgctcat tttctcttta cctcattaac tcaaatgagt tttgatttaa tttcagcggc
    10741 cagcgcctgg acctcgcggg cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc
    10801 ggcggcggca gtgcctgggt agctcacgcg ctgcgtgata cgggactcaa gaatgggcag
    10861 ctcgtacccg gccagcgcct cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg
    10921 cgacacgaca aaggccgctt gtagccttcc atccgtgacc tcaatgcgct gcttaaccag
    10981 ctccaccagg tcggcggtgg cccatatgtc gtaagggctt ggctgcaccg gaatcagcac
    11041 gaagtcggct gccttgatcg cggacacagc caagtccgcc gcctggggcg ctccgtcgat
    11101 cactacgaag tcgcgccggc cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat
    11161 gccgacaacg gttagcggtt gatcttcccg cacggccgcc caatcgcggg cactgccctg
    11221 gggatcggaa tcgactaaca gaacatcggc cccggcgagt tgcagggcgc gggctagatg
    11281 ggttgcgatg gtcgtcttgc ctgacccgcc tttctggtta agtacagcga taaccttcat
    11341 gcgttcccct tgcgtatttg tttatttact catcgcatca tatacgcagc gaccgcatga
    11401 cgcaagctgt tttactcaaa tacacatcac ctttttagac ggcggcgctc ggtttcttca
    11461 gcggccaagc tggccggcca ggccgccagc ttggcatcag acaaaccggc caggatttca
    11521 tgcagccgca cggttgagac gtgcgcgggc ggctcgaaca cgtacccggc cgcgatcatc
    11581 tccgcctcga tctcttcggt aatgaaaaac ggttcgtcct ggccgtcctg gtgcggtttc
    11641 atgcttgttc ctcttggcgt tcattctcgg cggccgccag ggcgtcggcc tcggtcaatg
    11701 cgtcctcacg gaaggcaccg cgccgcctgg cctcggtggg cgtcacttcc tcgctgcgct
    11761 caagtgcgcg gtacagggtc gagcgatgca cgccaagcag tgcagccgcc tctttcacgg
    11821 tgcggccttc ctggtcgatc agctcgcggg cgtgcgcgat ctgtgccggg gtgagggtag
    11881 ggcgggggcc aaacttcacg cctcgggcct tggcggcctc gcgcccgctc cgggtgcggt
    11941 cgatgattag ggaacgctcg aactcggcaa tgccggcgaa cacggtcaac accatgcggc
    12001 cggccggcgt ggtggtgtcg gcccacggct ctgccaggct acgcaggccc gcgccggcct
    12061 cctggatgcg ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg cggtctagcc
    12121 tggtcactgt cacaacgtcg ccagggcgta ggtggtcaag catcctggcc agctccgggc
    12181 ggtcgcgcct ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca
    12241 gttcggcccg ttggttggtc aagtcctggt cgtcggtgct gacgcgggca tagcccagca
    12301 ggccagcggc ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg caagtattct
    12361 actttatgcg actaaaacac gcgacaagaa aacgccagga aaagggcagg gcggcagcct
    12421 gtcgcgtaac ttaggacttg tgcgacatgt cgttttcaga agacggctgc actgaacgtc
    12481 agaagccgac tgcactatag cagcggaggg gttggatcaa agtactttaa agtactttaa
    12541 agtactttaa agtactttga tcccgagggg aaccctgtgg ttggcatgca catacaaatg
    12601 gacgaacgga taaacctttt cacgcccttt taaatatccg attattctaa taaacgctct
    12661 tttctcttag
//
