LOCUS       sequence-39122-e         699 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Lin CW, Ting AY
  TITLE     A genetically encoded fluorescent reporter of histone 
            phosphorylation in living cells.
  JOURNAL   Angew Chem Int Ed Engl. 2004 May 24;43(22):2940-3.
  PUBMED    15170310
REFERENCE   2  (bases 1 to 699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Angew Chem 
            Int Ed Engl."; date: "2004-05-24"; volume: "43(22)"; pages: "2940-3"
COMMENT     Sequence Label: pRSETB-S28 histone 3 phosphorylation reporter
FEATURES             Location/Qualifiers
     source          1..699
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(34..285)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(76..97)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     425..444
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 cttgtagcag ccggatcaag cttcgaattc ttacttgtac agctcgtcca tgccgagagt
       61 gatcccggcg gcggtcacga actccagcag gaccatgtga tcgcgcttct cgttggggtc
      121 tttgctcagg gcggactggt agctcaggta gtggttgtcg ggcagcagca cggggccgtc
      181 gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt
      241 gtggcggatc ttgaagttca ccttgatgcc gttcttctgc ttgtcggcca tgatatagac
      301 gttgtggctg ttgtagttgt actccagctt gtgccccagg atgttgccgt cctccttgaa
      361 gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg ccctcgaact tcacctcggc
      421 gcgggtcttg tagttgccgt cgtccttgaa gaagatggtg cgctcctgga cgtagccttc
      481 gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc gggcgaagca
      541 catcaggccg tagccgaagg tggtcacgag ggtgggccag ggcacgggca gcttgccggt
      601 ggtgcagatg aacttcaggg tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga
      661 cacgctgaac ttgtggccgt ttacgtcgcc gtccagctc
//
