LOCUS       sequence-391243-        4160 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick (MoClo) Level 0 Part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4160)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 4160)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pMC-6-TaGRF-GIF-9
FEATURES             Location/Qualifiers
     source          1..4160
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2040..2058)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2126..2230
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2452..3243
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3414..4002
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3903..3922
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(4156..4160,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcaa atggcgatgc cgtatgcctc tctttccccg
      121 gcaggcgacc gccgctcctc cccggccgcc accgccaccg cctccctcct cccattctgc
      181 cgctcctccc ccttctccgc cggcggcaat ggcggcatgg gggaggaggc gaggatggac
      241 gggaggtgga tggcgaggcc ggtgcccttc acagcggcgc agtacgagga gctggagcac
      301 caggcgctca tatacaagta cctggtggcc ggcgtgtccg tcccgccgga tctcgtgctc
      361 ccaatccgcc gcggcatcga gtccctcgcc gcccgcttct accacaaccc cctcgccatc
      421 gggtacggat cttacctggg caagaaggtg gatccggagc cgggccggtg caggcgcacg
      481 gacggcaaga agtggcggtg cgccaaggag gccgcctccg actccaagta ttgcgagcgc
      541 cacatgcacc gcggccgcaa cagatcaaga aagcctgtgg aaacgcagct cgtgtcgcac
      601 tcccagccgc cggccgcctc cgtcgtgccg cccctcgcca ccggcttcca caaccactcc
      661 ctctacccag ccatcggcgg caccaacggt ggtggaggcg gggggaacaa cggcatgtcc
      721 atgcccggca cattctcctc cgcgctgggg ccgcctcagc agcacatggg caacaatgcc
      781 gcctctccct acgcggctct cggcggcgcc ggaacatgca aagatttcag gtataccgca
      841 tatggaataa gatctttggc agacgagcag agtcagctca tgacagaagc catgaacacc
      901 tccgtggaga acccatggcg cctgccgcca tcttctcaaa cgactacatt cccgctctca
      961 agctactctc ctcagcttgg agcaacgagt gacctgggtc agaacaacag cagcaacaac
     1021 aacagcggcg tcaaggccga gggacagcag cagcagcagc cgctctcctt cccggggtgc
     1081 ggcgacttcg gcagcggcga ctccgcgaag caggagaacc agacactgag gccgttcttc
     1141 gacgagtggc caaagacgag ggactcgtgg tctgacctga ccgacgacaa ctcgaacgtc
     1201 gcctccttct cggccaccca gctgtctatc tcgataccaa tgacgtcctc cgacttctcc
     1261 gccgccagct cccagtcgcc caacggcatg ctgttcgccg gcgaaatgta cgcggccgct
     1321 gccatgcagc agcaacacct gatgcagatg aaccagagca tgatgggggg ctacgcttcc
     1381 tctaccactg ccaccactga tctcattcag cagtacctgg atgagaacaa gcagctgatc
     1441 ctggccatcc tcgacaacca gaacaacggc aaggtggagg agtgcgcacg gaaccaagct
     1501 aagctccagc agaacctcat gtacctcgcc gccatcgccg acagccagcc tccgcagaca
     1561 gcatctctgt ctcagtaccc gtccaacctg atgatgcagt ccgggccgcg gtacatgcag
     1621 cagcagtcgg cgcagatgat gtcgccgcag tctctgatgg cggcgaggtc gtcgatgatg
     1681 tacgcgcagc aggccatgtc tccgctccag caacagcagc aacaacagca gcaccaggcg
     1741 gccgcgcacg gccagctggg gatgtcctcc ggcgcgacca ccgggttcaa cctcctgcac
     1801 ggtgaggcca gcatgggcgg cggcggcggc gccactggca acagcatgat gaacgccagc
     1861 gtgttctcgg actatggccg cggcggcagc ggcgccaagg aggggtcgac ctcgctgtcg
     1921 gccgacgctc gcggcgccaa ctctggcgcg cacagcggcg acggggagta cctcaagggc
     1981 accgaggagg aaggaagcta gctttgagac cacgaagtgg ctcttcagtg gacgaaaggg
     2041 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     2101 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     2161 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     2221 aaggaagagt atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa
     2281 cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttttggggt acagtctatg
     2341 cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc
     2401 agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat catgagggaa
     2461 gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat
     2521 ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag
     2581 ccacacagcg atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg
     2641 cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc
     2701 cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct
     2761 aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag
     2821 ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt
     2881 gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt
     2941 gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag
     3001 cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg
     3061 ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc
     3121 atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca
     3181 gatcagttgg aagaatttgt ccattacgta aaaggcgaga tcaccaaggt agtcggcaaa
     3241 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     3301 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     3361 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     3421 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     3481 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     3541 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     3601 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     3661 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     3721 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     3781 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     3841 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     3901 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     3961 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     4021 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     4081 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     4141 cgaacgaccg agcgcagcga
//
