LOCUS       sequence-391245-        2972 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick (MoClo) Level 0 Part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 2972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pMC-6-IPT-9
FEATURES             Location/Qualifiers
     source          1..2972
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(852..870)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        938..1042
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1264..2055
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2226..2814
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2715..2734
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(2968..2972,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcaa atggatctgc gtctaatttt cggtccaact
      121 tgcacaggaa agacgtcgac cgcgatacgt cttgcccagc agactggcct tccagtcctt
      181 tcgctcgatc gggtccaatg ctgtcctcaa ctgtcaaccg gaagcggacg accaacagtg
      241 gaagaactga aaggaacgac ccgtctatac cttgaagatc ggcctctggt gaagggtatc
      301 atcgcagcca agcaagctca cgaaaggctg atcggggaag tgtacaatta tgaggcccac
      361 ggcgggctta ttcttgaggg aggatctatc tcgttgctca ggtgcatggc gcaaagcagt
      421 tattggagta ccgattttcg ttggcatatt attcgccaca agttagcaga cgaggagaca
      481 ttcatgaacg cggccaaggc cagagttagg cagatgttgc gccctgctgt aggcccatct
      541 attattcaag agttggttca tctttggaat gagcctcggc tgaggcccat actgaaagag
      601 atcgacggat atcgatatgc catgttattt gctagccaga accagatcac acccgatatg
      661 ctattgcagc ttgacccaga tatggagggt gagttgattc atggaatcgc tcaggagtat
      721 ctcatccatg cgcgccggca ggagcaggaa ttccctccag tgagcgtggt cgctttcgaa
      781 ggattcgaag gtccaccgtt cggaatgtgc tagctttgag accacgaagt ggctcttcag
      841 tggacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
      901 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
      961 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     1021 ataatattga aaaaggaaga gtatgcgctc acgcaactgg tccagaacct tgaccgaacg
     1081 cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttttggg
     1141 gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg
     1201 atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac
     1261 atcatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc
     1321 atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat
     1381 ggcggcctga agccacacag cgatattgat ttgctggtta cggtgaccgt aaggcttgat
     1441 gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag
     1501 agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg
     1561 cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca
     1621 ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga
     1681 gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa
     1741 caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg
     1801 gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc
     1861 ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag
     1921 tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg
     1981 gcctcgcgcg cagatcagtt ggaagaattt gtccattacg taaaaggcga gatcaccaag
     2041 gtagtcggca aataactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     2101 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     2161 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     2221 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     2281 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     2341 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     2401 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     2461 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     2521 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     2581 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     2641 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     2701 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     2761 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     2821 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     2881 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     2941 gctcgccgca gccgaacgac cgagcgcagc ga
//
