LOCUS       sequence-39125-e         947 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Certo MT, Ryu BY, Annis JE, Garibov M, Jarjour J, Rawlings DJ, 
            Scharenberg AM
  TITLE     Tracking genome engineering outcome at individual DNA breakpoints.
  JOURNAL   Nat Methods. 2011 Jul 10;8(8):671-6. doi: 10.1038/nmeth.1648.
  PUBMED    21743461
REFERENCE   2  (bases 1 to 947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-07-10- 10"; volume: "8"; 
            issue: "8"; pages: "671-6"
COMMENT     Sequence Label: pCVL.SFFV.d14GFP.EF1a.HA.NLS.Sce(opt).T2A.TagBFP
FEATURES             Location/Qualifiers
     source          1..947
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        3..214
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      223..232
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             238..264
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             238..264
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             274..294
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
       61 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
      121 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
      181 tctttttcgc aacgggtttg ccgccagaac acaggatctg ccgccaccat gggcgtatac
      241 ccctacgacg tgcccgacta cgcccccggg ccccctaaga aaaagaggaa ggtgaagaac
      301 atcaagaaga accaggtcat gaacctgggc cccaacagca agctgctgaa ggagtacaag
      361 agccagctga tcgagctgaa catcgagcag ttcgaggccg gcatcggcct gatcctgggc
      421 gacgcctaca tcaggagcag ggacgagggc aagacctact gcatgcagtt cgagtggaag
      481 aacaaggcct acatggacca cgtgtgcctg ctgtacgacc agtgggtgct gagccccccc
      541 cacaagaagg agagggtgaa ccacctgggc aacctggtca tcacctgggg cgcccagacc
      601 ttcaagcacc aggccttcaa caagctggcc aacctgttca tcgtgaacaa caagaagacc
      661 atccccaaca acctggtgga gaactacctg acccccatga gcctggccta ctggttcatg
      721 gacgacggcg gcaagtggga ctacaacaag aacagcacca acaagagcat cgtgctgaac
      781 acccagagct tcaccttcga ggaggtggag tacctggtga agggcctgag gaacaagttc
      841 cagctgaact gctacgtgaa gatcaacaag aacaagccca tcatctacat cgacagcatg
      901 agctacctga tcttctacaa cctgatcaag ccctacctga tccccca
//
