LOCUS       sequence-391250-        2503 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick (MoClo) Level 0 Part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pMC-9-AtHSP-11
FEATURES             Location/Qualifiers
     source          1..2503
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      95..343
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     primer_bind     complement(383..401)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        469..573
                     /gene="bla"
                     /label=AmpR promoter
     CDS             795..1586
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1757..2345
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2246..2265
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(2499..2503,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcag cttatatgaa gatgaagatg aaatatttgg
      121 tgtgtcaaat aaaaagcttg tgtgcttaag tttgtgtttt tttcttggct tgttgtgtta
      181 tgaatttgtg gctttttcta atattaaatg aatgtaagat cacattataa tgaataaaca
      241 aatgtttcta taatccattg tgaatgtttt gttggatctc ttctgcagca tataactact
      301 gtatgtgcta tggtatggac tatggaatat gattaaagat aagcgcttga gaccacgaag
      361 tggctcttca gtggacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
      421 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
      481 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
      541 taaatgcttc aataatattg aaaaaggaag agtatgcgct cacgcaactg gtccagaacc
      601 ttgaccgaac gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt
      661 ttttttttgg ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg
      721 gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa
      781 caaagttaaa catcatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg
      841 tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct
      901 ccgcagtgga tggcggcctg aagccacaca gcgatattga tttgctggtt acggtgaccg
      961 taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt
     1021 cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca
     1081 tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg
     1141 acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga
     1201 caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc
     1261 cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc
     1321 cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca
     1381 gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc
     1441 tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag
     1501 aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccattac gtaaaaggcg
     1561 agatcaccaa ggtagtcggc aaataactgt cagaccaagt ttactcatat atactttaga
     1621 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     1681 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     1741 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     1801 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     1861 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     1921 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     1981 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     2041 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     2101 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     2161 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     2221 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     2281 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     2341 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     2401 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     2461 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cga
//
