LOCUS       sequence-391252-        2554 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick (MoClo) Level 0 Part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pMC-9-SlUbi-11
FEATURES             Location/Qualifiers
     source          1..2554
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(434..452)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        520..624
                     /gene="bla"
                     /label=AmpR promoter
     CDS             846..1637
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1808..2396
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2297..2316
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(2550..2554,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcag cttgttgtgg ttgtctggtt gcgtctgttg
      121 cccgttgtct gttgcccatt gtggtggttg tgtttgtatg atggtcgtta aggatcatca
      181 atgtgttttc gctttttgtt ccattctgtt tctcatttgt gaataataat ggtatcttta
      241 tgaatatgca gtttgtggtt tcttttctga ttgcagttct gagcattttg tttttgcttc
      301 cgtttactat accacttaca gtttgcacta atttagttga tatgcgagcc atctgatgtt
      361 tgatgattca aatggcgttt atgtaactcg tacccgcttg agaccacgaa gtggctcttc
      421 agtggacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
      481 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
      541 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
      601 caataatatt gaaaaaggaa gagtatgcgc tcacgcaact ggtccagaac cttgaccgaa
      661 cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttttg
      721 gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt
      781 tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa
      841 acatcatgag ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg
      901 tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg
      961 atggcggcct gaagccacac agcgatattg atttgctggt tacggtgacc gtaaggcttg
     1021 atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag
     1081 agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt
     1141 ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg
     1201 caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa
     1261 gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg
     1321 aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact
     1381 gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa
     1441 ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc
     1501 agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct
     1561 tggcctcgcg cgcagatcag ttggaagaat ttgtccatta cgtaaaaggc gagatcacca
     1621 aggtagtcgg caaataactg tcagaccaag tttactcata tatactttag attgatttaa
     1681 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     1741 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     1801 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     1861 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     1921 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     1981 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     2041 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     2101 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     2161 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     2221 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     2281 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     2341 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     2401 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     2461 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     2521 ccgctcgccg cagccgaacg accgagcgca gcga
//
