LOCUS       sequence-391254-        2694 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick (MoClo) Level 0 Part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pMC-1-AtU6-Y
FEATURES             Location/Qualifiers
     source          1..2694
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(574..592)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        660..764
                     /gene="bla"
                     /label=AmpR promoter
     CDS             986..1777
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1948..2536
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2437..2456
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(2690..2694,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcag gagcttcgtt gaacaacgga aactcgactt
      121 gccttccgca caatacatca tttcttctta gctttttttc ttcttcttcg ttcatacagt
      181 ttttttttgt ttatcagctt acattttctt gaaccgtagc tttcgttttc ttctttttaa
      241 ctttccattc ggagtttttg tatcttgttt catagtttgt cccaggatta gaatgattag
      301 gcatcgaacc ttcaagaatt tgattgaata aaacatcttc attcttaaga tatgaagata
      361 atcttcaaaa ggcccctggg aatctgaaag aagagaagca ggcccattta tatgggaaag
      421 aacaatagta tttcttatat aggcccattt aagttgaaaa caatcttcaa aagtcccaca
      481 tcgcttagat aagaaaacga agctgagttt atatacagct agagtcgaag tagtgatttg
      541 agaccacgaa gtggctcttc agtggacgaa agggcctcgt gatacgccta tttttatagg
      601 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
      661 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
      721 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgcgc tcacgcaact
      781 ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt
      841 gttatgactg tttttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg
      901 ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag
      961 tcgccctaaa acaaagttaa acatcatgag ggaagcggtg atcgccgaag tatcgactca
     1021 actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca
     1081 tttgtacggc tccgcagtgg atggcggcct gaagccacac agcgatattg atttgctggt
     1141 tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga
     1201 aacttcggct tcccctggag agagcgagat tctacgcgct gtagaagtca ccattgttgt
     1261 gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg
     1321 gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc
     1381 tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga
     1441 actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct
     1501 atggaactcg ccgccagact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg
     1561 catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc
     1621 aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct
     1681 tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccatta
     1741 cgtaaaaggc gagatcacca aggtagtcgg caaataactg tcagaccaag tttactcata
     1801 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     1861 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttacact gagcgtcaga
     1921 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     1981 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     2041 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     2101 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     2161 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     2221 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     2281 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     2341 atgagaaagc gccacgcttc cagaagggag aaaggcggac aggtatccgg taagcggcag
     2401 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     2461 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     2521 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     2581 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     2641 agcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcga
//
