LOCUS       sequence-391265-       12577 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BRET lux luciferase with cp157Venus for mammalian expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12577)
  AUTHORS   Kaku T, Sugiura K, Entani T, Osabe K, Nagai T
  TITLE     Enhanced brightness of bacterial luciferase by bioluminescence 
            resonance energy transfer.
  JOURNAL   Sci Rep. 2021 Jul 22;11(1):14994. doi: 10.1038/s41598-021-94551-4.
  PUBMED    34294849
REFERENCE   2  (bases 1 to 12577)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2021-07-22"; volume: "11(1):14994. doi"; pages: " 
            10.1038/s41598-021-94551-4"
COMMENT     Sequence Label: pCMVlux-cp157Venus
FEATURES             Location/Qualifiers
     source          1..12577
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(19..38)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     265..285
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(294..722)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(404..425)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     616..635
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    768..992
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     981..998
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(1748..1801)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(2948..3004)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(join(3035..3493,3494..3496,3497..3499))
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITA"
     primer_bind     3174..3193
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     complement(3557..3578)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(4748..4801)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(5915..5974)
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     regulatory      8458..8467
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    8462..8624
                     /label=QBI SP163
                     /note="strong translational enhancer derived from the 5' 
                     UTR of the mouse VEGF gene"
     primer_bind     complement(8653..8677)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(8674..8885)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(8703..8723)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        8886..9265
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9485..9589
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9590..9658,9659..10450)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9808..9827)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10621..11209
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11110..11129
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11363..11380
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    11413..11534
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11424..11443)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     11478..11497
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(11708..12502)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MVEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(11819..11838)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     12429..12448
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(join(12528..12577,1..280))
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(12542..12577,1..100)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 gctcagaggc agaggcggcc tcggcctctg cataaataaa aaaaattagt cagcctaggg
       61 gcggagaatg ggcggaactg ggcggagtta ggggcgggat gggcggagtt aggggcggga
      121 ctatggttgc tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg
      181 gggactttcc acacctggtt gctgactaat tgagatgcat gctttgcata cttctgcctg
      241 ctggggagcc tggggacttt ccacacccta actgacacac attccacaga attaattcgc
      301 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
      361 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
      421 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
      481 tggcccacta cgtgaaccat caccctaatc aagttttttg ggggagaggt gccgtaaagc
      541 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
      601 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
      661 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
      721 gtggggatac cccctagagc cccagctggt tctttccgcc tcagaagcca tagagcccgg
      781 gccatcccca gcatgcctgc tattgtcttc ccaatcctcc cccttgctgt cctgccccac
      841 cccacccccc agaatagaat gacacctact cagacaatgc gatgcaattt cctcatttta
      901 ttaggaaagg acagtgggag tggcaccttc cagggtcaag gaaggcacgg gggaggggca
      961 aacaacagat ggctggcaac tagaaggcac agtcgaggct gatcagcgag cttctagatt
     1021 atctcttggc caggcccttg ctgttcaggt agggcagaat gtggggtctg ctctcgccag
     1081 cgagcttgcc ggtgacttcc tgggaccagg tgctcagctt ctggttgctg gttctgctgg
     1141 cgtagtaggc ctgcatggtc tggtcgtagc tctggatgtc gtccaggttc agttcctggt
     1201 actgattctc gtgcaccacc acgtgggcgg gcagtctggg cttcacttcg gggttctggt
     1261 cggggtggcc caggcacatg ccgaacagca cggcgctgtt ctcaggcagc cccagcagct
     1321 cgtccacctg ggcggcagag tttctcaggc cgccgatgta cacgccgccc aggcccatag
     1381 actcggcagc cagcaggcaa ttctgggcca tgatgccgga gtccacggcg ccgatcagtg
     1441 tcagctcggt gaaatcggcc tgcacgtcgg ggttgattgt ggcgtgccgc tggtagtcga
     1501 tgcagaacac cagaaactcg gcggcagatt ccacgtaggc ctggttgccg gcgaactggg
     1561 ccagctcatt ccgcttctcg ctgtcggtca cgcgcacgat ggacaccacc tgcagcatag
     1621 agctggagct ggcagccagt ccggcctgga tgatggtctg tctctgctcg tcggtgatgg
     1681 gcacggcggt aaacttccgg atggaccggt gggccaggat tgtctcgatg gtgttgttca
     1741 ttcgcgaagg gccgggattc tcctccacgt caccgcatgt tagaagactt cctctgccct
     1801 caccgccccc gctgcctcca ccgttaacgc tgtcgaaggc ctctgtcagg ctcagggcgc
     1861 agcccttctg ggtccgggtg ttcactctcc gcaggatttc caccagcacg ccggggtact
     1921 tgccgtactc gcggctgatg atgcccacgt cgtcggtcac gataaaggcg gggtagctgg
     1981 tggcgctggc gtccatgtag ctcatcaggc caggggtgcc atcaggcacg ggcttcagtg
     2041 tctcggggtc cagggctcta gcgtacaccc aggggggcac gtgcttccgc tgcatctcgt
     2101 cctcgaagaa gcaggtattc agttccacct ggttgaagat gtcccggatc tgggagatgt
     2161 cgctcaggtt gaaggtgtcg aacagcagat gattgaagtc gtcccgcttc agggactctt
     2221 tctcgtagga cttccatccg ccgcctgtga tgatgtacag ggacttgtcg ccgctgaagg
     2281 agatcttctt gtccttcatg tagtggcaca gcaggtagat gaagtagggg gagccgatca
     2341 ggcacaggtc cttgccctgg ttcttgatcc gttccaggga gttcagggtt ttcacgaagt
     2401 cgatccgttc ctcggtcacg gtgaaggtgg tggggtacag cagttccacc aggctcatca
     2461 cgtatttgaa ccagatattg tgggcgttga accggtcggg tcccaggttg accagctcga
     2521 tctggtgatc gaaccaactg cccacgtact tcatgccgta ggacacggag cccagcagtc
     2581 tctcgatgct cagccggtcc cgggccacct gggacttcag gccgttggtg ccggagctgg
     2641 tgaaccagga ctcgatctcg ttttcctggc tggtcagcag ccgggtgaac ttgaacacgc
     2701 tggtagggaa cacggggatg tcatcgatct cggtgatatt atcgtccacc ttgtgggcct
     2761 ggcagtagtg ccggtactct ctgcagtgct tgtagtggtt ccggaaggcg tccagcacca
     2821 gtttcttccg gatcttttcc tgctcgtcgt aggaccagac cagggggtcg ctgctgaaga
     2881 tcagatcgtc gatctcgctg ctggcggtaa tttcctgctt gtccacgtag ctggtcatcc
     2941 acccgcgtgg tccgggattc tcctcaacgt caccagcttg cttcagcaag ctaaagtttg
     3001 tcgcgccacc ccctgatccc ccaccgatct tgtcggcggt gatatagacg ttgtggctgt
     3061 tgtagttgta ctccagcttg tgccccagga tgttgccgtc ctccttgaag tcgatgccct
     3121 tcagctcgat gcggttcacc agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt
     3181 agttgccgtc gtccttgaag aagatggtgc gctcctggac gtagccttcg ggcatggcgg
     3241 acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg ggcgaagcac tgcaggccgt
     3301 agcccagggt ggtcacgagg gtgggccagg gcacgggcag cttgccggtg gtgcagatca
     3361 gcttcagggt cagcttgccg taggtggcat cgccctcgcc ctcgccggac acgctgaact
     3421 tgtggccgtt tacgtcgccg tccagctcga ccaggatggg caccaccccg gtgaacagct
     3481 cctcgccctt gctcaccatg ccaccgctgc cacccttgta cagctcgtcc atgccgagag
     3541 tgatcccggc ggcggtcacg aactccagca ggaccatgtg atcgcgcttc tcgttggggt
     3601 ctttgctcag ggcggactgg tagctcaggt agtggttgtc gggcagcagc acggggccgt
     3661 cgccgatggg ggtgttctgc tggtagtggt cggcgagctg cacgccgccg tcctcgatgt
     3721 tgtggcggat cttgaagttg gccttgatgc cgttcttctg catgagatcc atatggtatt
     3781 tcttgatgtt gtcgtccacg atgttgatca cgtttttctg gtgcatcagg tcgttcatgg
     3841 gctcgaagct cagcagcacc cgcttggcgc cgcacttctc gatggccagc ttggcagcgg
     3901 cgatacactc ggtgtagtcg cccacggcgt tctcggtgat gatctcttcc agcttgtttt
     3961 ccaggttctc gttggggtac atttccagca cgtagtcccg gatgaaggcc cgggtttcct
     4021 gcttagcctt gtggctgtcc tcgttgtagt tcaccagcac catcagctgg tggtcgatgg
     4081 cgctcaggtc gatgccgtac ttatcggcca cggccttgta ccgctcggcg tactcgtagc
     4141 gcacgtcgtt gctgtcgtcc cacttgaaga tcagggggat gcccttcttg gcggcccatt
     4201 ccacgatgtg gtggctggtg gcggtgatgt accgtctggg tccgccctgg gtgtaggcgt
     4261 gggggttcac gctgatcttg gggaagctgt agaagtcgtt gtcggggttg cagtagccgg
     4321 tggtcagggc gtcgttgatg atctcgtagc attcctcgaa cagctgctgc tggtattcca
     4381 cgggccggtt gaacagccgc atctcgtctt tcttctcgca gtcgctgaag cccaggatga
     4441 accggccctc gctcagctga tccagcaggc aggcttcctc ggcgatccgc acggggtggt
     4501 gggtggtgat gatgtggttc aggctgccga tcttgatctt ctcggtcagg cccagcagga
     4561 agccagacac ggtcagaggg gctccgacca cgccgttgcc gctgaagtgg ttctcgtaca
     4621 ccaggatctg ctcgaagttc agcttgtcaa cgtactcggt gatttcctgc atccggacga
     4681 tgctctgttc ctgcacggtg gtactgttga tgaagttcag gaagaacagg ccgaacttca
     4741 tagcgctggg tcctgggttt tcttcgacat ctccacaggt caataatgaa cccctacctt
     4801 ctcctccgcc tgaaccgcca ccctggtaca gcaggctccg ctgcttctct ttcaggaagg
     4861 gcatcacgtc gctctggaac agcttcatgc tggcgataat ctcgtccacg gtgccgttgg
     4921 cctcaaagcc gcagcagatg ttgctgatgc cggtggcgtc gatgtctttc tggatgatgt
     4981 cgatgcattc ctggggggtg cccacagggt tgatctcgta gctgtagtcg atccgccggt
     5041 tggtgtcctt gtgtcccttc agcacgaagt cccgccactg gcccttgttg aagtcgtagc
     5101 cccgggtctg gtcgctgtcg tcgaagatgg tggtggcgtt cacgtaggag tcgtaccaat
     5161 ggcccaggaa cttccggcag atctctttgg ccttgatgct gtcgtggtcc acgctggtaa
     5221 tgtagctcag gcagtggtcg atgttgtgga tgtcgtggcc gtattcctgg gccacctcgt
     5281 tgtacagttc cagctgggcc ttcttctcgt tggtgttgat gatccagctc aggatcatgg
     5341 gcaggccaaa ctgagcggcc cactctgtgg tgctggcgct ctcggccacc acgtacacag
     5401 gggctccgcc tctgctgtag gcggcagggt tcactttcac tttgtggaac ttgatgtgct
     5461 cgttgtcggc ttccatgtag ccctcggtca tgccgttctt gatcaggccg taccaacatt
     5521 cggccagggc tctggagttg ttcatgtcgg tgccgaacac ccggaagtcc ttgttgtaca
     5581 ggcctctgca gatgccgaat ctgaaccggc ccttagacat ctggtcgagc aggttcacat
     5641 cttccagctg ccgcacaggg tgagctgtag gcagcacgat ggcggcggtg cccacgttca
     5701 gtttcttggt ggcgcccagc aggtaggcag cggccacgta ggggtttccc agcaggccga
     5761 actcggtgaa gtggtgttcc agcagccaca cggtgtcgaa gccgcattcc tcgctgatcc
     5821 ggcccagttt gaccagtctc ttcatcactt cggtctggct gaactggggg ggctggtagg
     5881 tcagcaggaa gttgccgaac ttcatattta aattaggacc ggggttactt tcaacatcgc
     5941 cagcgagttt caacaaagcg tagttagtac actggccccc tcctgaacct cccccgttta
     6001 aaccggacag gctgatggcc tggttctcga tctcgatccg catccgccgc tcgttcacgg
     6061 tggcgattgt caggtgctcg aagctgggct cggtgatgtc cacgtcaatg tccaggtggt
     6121 cgttgtccat agcaatggcg gccttggtca cgctctggta gaagttccgc agcaccacca
     6181 ggttctcgct caggtcgtgg gagctgccca gcaggctgta gatcttgcac cggttggacc
     6241 ggatgttgct cagcagggtg atcacttcgt cctgtttgac ccagttgtcg ttgttggcgg
     6301 taaaggcgat aaaggggatg tccaggtaca tcatattgtt gatggtgctg gccaggtcct
     6361 cccagccgaa atccaggcag tcccgggcga acacctcggc gcccagcttg tggccctcga
     6421 agtccaggtt gtcgggcagc tcattgatgg gcaggctcag gtagtcgaag cccagggccc
     6481 gttccaggct gtaccgcagg ttgaccacgc ccacggcggt aatcaggaag ctggcgttga
     6541 tctcggacag gctggcgtag gcgattctgg cgctcaggct agaggccagc atgccgaagt
     6601 tgttgatctt ccgggtggtc agccagtcca ccacggccag cagagactgc ttgccgatgc
     6661 tcatggtgaa ctcgtcgatg gtgccgctgc tcaggcccac atggtgcagg ctgtcgtatc
     6721 tgatcacgtg gaagccgttc cggctcaggt actcggccag gccggcaaag tggtccatcc
     6781 gtctggcgaa gccgctggca atgatgatgg cgttcttccg cttggggctg ttttcctcgg
     6841 gcagtgtctc ccacacgtgg attttcttgt tgccttccac gcagatcacg tggtcgatgg
     6901 tcttgtactt gctctcattt tccatgagct cgggccctgg gttggactcc acgtctcccg
     6961 ccaacttgag aaggtcaaaa ttcaacagct gcccaccgcc tgatccgcca cctacgtagg
     7021 gcacgaacac caggaacttg tcctcttcca ggaaccgggt ctgctcgatt tccacggcca
     7081 cgtccttggc ggtgtagttg ctgggccgct cgtggctgat atatgtgacc agccgctgca
     7141 gaggcctcat gccgtcgtgg ctgccgccca ctctgaagat gttgttcatg ccagcttcca
     7201 cgattctctc ggcgcccttc agagccaggg cgtcccggta tttgaaggag gattcccaag
     7261 ggaagatgct gatggtctgg gtcttgttct tctgcacgta gggcagaatc tgctcgatgt
     7321 tgtccacgtg gtgcaggtac acacatctgc ccaggggctg gttgaattcc acgccggcgt
     7381 tgctctcgat gatcatccat ctctggtgga tgtccacttc caccttcagg ccggcgaaca
     7441 ggctctcttt ctgcaccaga gagtaggcgg ccttctcgtc gaagtccttc ttggcgttgg
     7501 gcaggatgtg ggcgtacagg ttcagcttct cgatcagggc cagcttgaat tcctcgtagt
     7561 ggttgcccat gtagtagatg ttctgggcgc tgaagcaggc ccgctggtcg tagaagcaca
     7621 cgtcgtgagc ggcgcctgtg gcggcgctgg tcaggtccac ggggttgtcg atgatgcaca
     7681 ggcttttctt gctgccgaac ttgatcacat cggcgtagct gggggcgtgt tccacggccc
     7741 aattgatggc gtcggggcct ccccaggcca cgatcacgtc ggcgtgccgc atgatttctt
     7801 tggccaggct ggtgtcgccc tggtggggcc agtagatcac gctcagggac cgggtgatgg
     7861 ggtggttggg gtccacgtcg atgaagctca gggccagggc attggcggtg aaggggtcgg
     7921 tgctgctggt cttgatgatg cactggttct tggtcaggat ggcccgcagg atgctcatga
     7981 tgccgctcag gggcacattg ccggccagca gatgcacgga cttgcccttg gggaaggccc
     8041 gcacgtagct ctcgtcctgg ggcagccact cgtccatgat gtgccggctg cccagctcgt
     8101 tttccaccac gtcgtacagg ccgcccttgc tgcacaggat catgctgatc cagttggctt
     8161 ccagcttggc catttcctcg ctgtagccca tgtacttttt caggtcccgg atgtaggttc
     8221 tccgccggct gtactcttcg ttcttccacc gctggcccac ggtgtacaga aagttcacga
     8281 tgttgtgcag ccgcagctcg ttgttgccgt tgcagtcgat gatgttcttc acgtggctgt
     8341 cgttcaggat gggcaggtac acgctgttgt cgccgaagtt gatgctctgc accaggtcgt
     8401 cgctctcggg gaagatttcc acctggccgt tgatgatgaa gctgatcttc ttggtgccca
     8461 tggtggcgga ggccgtccgg ggccggcgcg gctcgcgctc cctctccggc tcggactgcg
     8521 aggcagcccg ctctcttgcg cgccttggcg ggctcctctc ccttctggaa ccgaggcccg
     8581 ggccggggcc tggctgccgc tcggctgccc caagcctctg cgctgctagc aagggcgaat
     8641 tctcggtcga ctgatctgac ggttcactaa acgagctctg cttatataga cctcccaccg
     8701 tacacgccta ccgcccattt gcgtcaacgg ggcgggcgat cgcagttgtt acgacatttt
     8761 ggaaagtccc gttgattttg gtgccaaaac aaactcccat tgacgtcaat ggggtggaga
     8821 cttggaaatc cccgtgagtc aaaccgctat ccacgcccat tggtgtactg ccaaaaccgc
     8881 atcaccatgg taatagcgat gactaatacg tagatgtact gccaagtagg aaagtcccgt
     8941 aaggtcatgt actgggcata atgccaggcg ggccatttac cgtcattgac gtcaataggg
     9001 ggcggacttg gcatatgata cacttgatgt actgccaagt gggcagttta ccgtaaatac
     9061 tccacccatt gacgtcaatg gaaagtccct attggcgtta ctatgggaac atacgtcatt
     9121 attgacgtca atgggcgggg gtcgttgggc ggtcagccag gcgggccatt taccgtaagt
     9181 tatgtaacgc ggaactccat atatgggcta tgaactaatg accccgtaat tgattactat
     9241 taataactag tcaataatca atgccaacat ggcggtcata ttggacatga gccaatataa
     9301 atgtacatat tatgatatag atacaacgta tgcaatggcc aatagccaat attgatttat
     9361 gctatataac caatgaataa tatggctaat ggccaatatt gaagatctgt cgagccatgt
     9421 acgcgtggta ccgatctccc gatccgtcga tctgacgtca ggtggcactt ttcggggaaa
     9481 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     9541 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     9601 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     9661 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgccc gagtgggtta
     9721 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     9781 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     9841 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     9901 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     9961 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
    10021 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
    10081 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
    10141 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
    10201 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
    10261 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
    10321 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
    10381 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
    10441 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
    10501 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
    10561 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
    10621 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
    10681 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
    10741 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
    10801 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
    10861 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
    10921 ggcgcagcgg tcgggctgaa cggggggttc gtgcatacag cccagcttgg agcgaacgac
    10981 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
    11041 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
    11101 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
    11161 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
    11221 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
    11281 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
    11341 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaaa gatcgacggt
    11401 atacagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
    11461 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
    11521 gcaataaaca agttggggtg ggcgaagaac tccagcatga gatccccgcg ctggaggatc
    11581 atccagccgg cgtcccggaa aacgattccg aagcccaacc tttcatagaa ggcggcggtg
    11641 gaatcgaaat ctcgtgatgg caggttgggc gtcgcttggt cggtcatttc gaaccccaga
    11701 gtcccgctca gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag
    11761 cggcgatacc gtaaagcacg aggaagcggt cagcccattc gccgccaagc tcttcagcaa
    11821 tatcacgggt agccaacgct atgtcctgat agcggtccgc cacacccagc cggccacagt
    11881 cgatgaatcc agaaaagcgg ccattttcca ccatgatatt cggcaagcag gcatcgccat
    11941 gagtcacgac gagatcctcg ccgtcgggca tacgcgcctt gagcctggcg aacagttcgg
    12001 ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg atcgacaaga ccggcttcca
    12061 tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg
    12121 gatcaagcgt atgcagccgc cgcattgcat cagccatgat ggatactttc tcggcaggag
    12181 caaggtgaga tgacaggaga tcctgccccg gcacttcgcc caatagcagc cagtcccttc
    12241 ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg gccagccacg
    12301 atagccgcgc tgcctcgtcc tggagttcat tcagggcacc ggacaggtcg gtcttgacaa
    12361 aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc ggcatcagag cagccgattg
    12421 tctgttgtgc ccagtcatag ccgaatagcc tctccaccca agcggccgga gaacctgcgt
    12481 gcaatccatc ttgttcaacc attgtggacc tcccgggggg ggagcttttt gcaaaagcct
    12541 aggcctccaa aaaagcctcc tcactacttc tggaata
//
