LOCUS       sequence-391267-        7335 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Synthetic gene circuit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7335)
  AUTHORS   Inda-Webb ME, Jimenez M, Liu Q, Phan NV, Ahn J, Steiger C, Wentworth
            A, Riaz A, Zirtiloglu T, Wong K, Ishida K, Fabian N, Jenkins J, 
            Kuosmanen J, Madani W, McNally R, Lai Y, Hayward A, Mimee M, Nadeau 
            P, Chandrakasan AP, Traverso G, Yazicigil RT, Lu TK
  TITLE     Sub-1.4 cm(3) capsule for detecting labile inflammatory biomarkers 
            in situ.
  JOURNAL   Nature. 2023 Aug;620(7973):386-392. doi: 10.1038/s41586-023-06369-x.
            Epub 2023 Jul 26.
  PUBMED    37495692
REFERENCE   2  (bases 1 to 7335)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-023-06369-x"; journalName: "Nature"; date: 
            "2023-08"; volume: "620"; issue: "7973"; pages: "386-392"
COMMENT     Sequence Label: NO- DSD Sensor 1
FEATURES             Location/Qualifiers
     source          1..7335
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1714..1800
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(2345..3295)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      3343..3565
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(4063..4157)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(4187..4978,4979..5047))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4810..4829
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5048..5151)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5624..7123
                     /codon_start=1
                     /transl_table=11
                     /gene="Bxb1"
                     /product="Bxb1 integrase"
                     /label=Bxb1 integrase
                     /note="Bxb1 large serine integrase from Mycobacterium 
                     bacteriophage Bxb1 (Ghosh et al. 2003)."
                     /protein_id="AFF61396.1"
                     /translation="MRALVVIRLSRVTDATTSPERQLESCQQLCAQRGWDVVGVAEDLD
                     VSGAVDPFDRKRRPNLARWLAFEEQPFDVIVAYRVDRLTRSIRHLQQLVHWAEDHKKLV
                     VSATEAHFDTTTPFAAVVIALMGTVAQMELEAIKERNRSAAHFNIRAGKYRGSLPPWGY
                     LPTRVDGEWRLVPDPVQRERILEVYHRVVDNHEPLHLVAHDLNRRGVLSPKDYFAQLQG
                     REPQGREWSATALKRSMISEAMLGYATLNGKTVRDDDGAPLVRAEPILTREQLEALRAE
                     LVKTSRAKPAVSTPSLLLRVLFCAVCGEPAYKFAGGGRKHPRYRCRSMGFPKHCGNGTV
                     AMAEWDAFCEEQVLDLLGDAERLEKVWVAGSDSAVELAEVNAELVDLTSLIGSPAYRAG
                     SPQREALDARIAALAARQEELEGLEARPSGWEWRETGQRFGDWWREQDTAAKNTWLRSM
                     NVRLTFDVRGGLTRTIDFGDLQEYEQHLRLGSVVERLHTGMS"
     terminator      7231..7317
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cacagctaac accacgtcgt ccctatctgc tgccctaggt ctatgagtgg ttgctggata
       61 actttacggg catgcataag gctcgtataa tatattcagg gagaccacaa cggtttccct
      121 ctacaaataa ttttgtttaa ctttgaattc ttcacacagg aaaccggtac catgagtttt
      181 tccgttgatg tgctggcgaa tatcgccatc gaattgcagc gtgggattgg tcaccaggat
      241 cgttttcagc gcctgatcac cacgctacgt caggtgctgg agtgcgatgc gtctgcgttg
      301 ctacgttacg attcgcggca gtttattccg cttgccatcg acggtctggc aaaggatgta
      361 ctcggtagac gctttgcgct ggaagggcat ccacggctgg aagcgattgc ccgcgccggg
      421 gatgtggtgc gctttcccgc agacagcgaa ttgcccgatc cctatgacgg tttgattcct
      481 gggcaggaga gtctgaaggt tcacgcctgc gttggtctgc cattgtttgc cgggcaaaac
      541 ctgatcggcg cactgacgct cgacgggatg cagcccgatc agttcgatgt tttcagcgac
      601 gaagagctac ggctgattgc tgcgctggcg gcgggagcgt taagcaatgc gttgctgatt
      661 gaacaactgg aaagccagaa tatgctgcca ggcgatgcca cgccgtttga agcggtgaaa
      721 cagacgcaga tgattggctt gtcccctggc atgacgcaac tgaaaaaaga gattgagatt
      781 gtggcggcgt ccgatctcaa cgtcctgatc agcggtgaga ctggaaccgg taaggagctg
      841 gtggcgaaag cgattcatga agcctcgcca cgggcggtga atccgctggt ctatctcaac
      901 tgtgctgcac tgccggaaag tgtggcggaa agtgagttgt tcgggcatgt gaaaggagcg
      961 tttactggcg ctatcagtaa tcgcagcggg aagtttgaaa tggcggataa cggcacgctg
     1021 tttctggatg agatcggcga gttgtcgttg gcattgcagg ccaagctgct gagggtgttg
     1081 cagtatggcg atattcagcg cgttggcgat gaccgttgtt tgcgggtcga tgtgcgcgtg
     1141 ctggcggcga ctaaccgcga tttacgcgaa gaggtgctgg cagggcgatt ccgcgccgat
     1201 ttgtttcatc gcctgagcgt gtttccactt tcggtgccgc cgctgcgtga gcggggcgat
     1261 gatgtcattc tgctggcggg gtatttctgc gagcagtgtc gtttgcggca ggggctctcc
     1321 cgcgtggtat taagtgccgg agcgcgaaat ttactgcaac actacagttt tccgggaaac
     1381 gtgcgcgaac tggaacatgc tattcatcgg gcggtagttc tggcgagagc cacccgcagc
     1441 ggcgatgaag tgattcttga ggcgcaacat tttgcttttc ctgaggtgac gttgccgacg
     1501 ccagaagtgg cggcggtgcc cgttgttaag caaaacctgc gtgaagcgac agaagcgttc
     1561 cagcgtgaaa ctattcgtca ggcactggca caaaatcatc acaactgggc tgcctgcgcg
     1621 cggatgctgg aaaccgacgt cgccaacctg catcggctgg cgaaacgtct gggattgaag
     1681 gattaaatct catggtacgc gtgctagagg catcaaataa aacgaaaggc tcagtcgaaa
     1741 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat
     1801 ccgccgccct agacctaggg cctagggtac gggttttgct gcccgcaaac gggctgttct
     1861 ggtgttgcta gtttgttatc agaatcgcag atccggcttc agccggtttg ccggctgaaa
     1921 gcgctatttc ttccagaatt gccatgattt tttccccacg ggaggcgtca ctggctcccg
     1981 tgttgtcggc agctttgatt cgataagcag catcgcctgt ttcaggctgt ctatgtgtga
     2041 ctgttgagct gtaacaagtt gtctcaggtg ttcaatttca tgttctagtt gctttgtttt
     2101 actggtttca cctgttctat taggtgttac atgctgttca tctgttacat tgtcgatctg
     2161 ttcatggtga acagctttga atgcaccaaa aactcgtaaa agctctgatg tatctatctt
     2221 ttttacaccg ttttcatctg tgcatatgga cagttttccc tttgatatgt aacggtgaac
     2281 agttgttcta cttttgtttg ttagtcttga tgcttcactg atagatacaa gagccataag
     2341 aacctcagat ccttccgtat ttagccagta tgttctctag tgtggttcgt tgtttttgcg
     2401 tgagccatga gaacgaacca ttgagatcat acttactttg catgtcactc aaaaattttg
     2461 cctcaaaact ggtgagctga atttttgcag ttaaagcatc gtgtagtgtt tttcttagtc
     2521 cgttatgtag gtaggaatct gatgtaatgg ttgttggtat tttgtcacca ttcattttta
     2581 tctggttgtt ctcaagttcg gttacgagat ccatttgtct atctagttca acttggaaaa
     2641 tcaacgtatc agtcgggcgg cctcgcttat caaccaccaa tttcatattg ctgtaagtgt
     2701 ttaaatcttt acttattggt ttcaaaaccc attggttaag ccttttaaac tcatggtagt
     2761 tattttcaag cattaacatg aacttaaatt catcaaggct aatctctata tttgccttgt
     2821 gagttttctt ttgtgttagt tcttttaata accactcata aatcctcata gagtatttgt
     2881 tttcaaaaga cttaacatgt tccagattat attttatgaa tttttttaac tggaaaagat
     2941 aaggcaatat ctcttcacta aaaactaatt ctaatttttc gcttgagaac ttggcatagt
     3001 ttgtccactg gaaaatctca aagcctttaa ccaaaggatt cctgatttcc acagttctcg
     3061 tcatcagctc tctggttgct ttagctaata caccataagc attttcccta ctgatgttca
     3121 tcatctgagc gtattggtta taagtgaacg ataccgtccg ttctttcctt gtagggtttt
     3181 caatcgtggg gttgagtagt gccacacagc ataaaattag cttggtttca tgctccgtta
     3241 agtcatagcg actaatcgct agttcatttg ctttgaaaac aactaattca gacatacatc
     3301 tcaattggtc taggtgattt taatcactat accaattgag atgggctagt caatgataat
     3361 tactagtcct tttcctttga gttgtgggta tctgtaaatt ctgctagacc tttgctggaa
     3421 aacttgtaaa ttctgctaga ccctctgtaa attccgctag acctttgtgt gttttttttg
     3481 tttatattca agtggttata atttatagaa taaagaaaga ataaaaaaag ataaaaagaa
     3541 tagatcccag ccctgtgtat aactcactac tttagtcagt tccgcagtat tacaaaagga
     3601 tgtcgcaaac gctgtttgct cctctacaaa acagacctta aaaccctaaa ggcttaagta
     3661 gcaccctcgc aagctcgggc aaatcgctga atattccttt tgtctccgac catcaggcac
     3721 ctgagtcgct gtctttttcg tgacattcag ttcgctgcgc tcacggctct ggcagcgaat
     3781 gggggtaaat ggcactacag gcgcctttta tggattcatg caaggaaact acccataata
     3841 caagaaaagc ccgtcacggg cttctcaggg cgttttatgg cgggtctgct atgtggtgct
     3901 atctgacttt ttgctgttca gcagttcctg ccctctgatt ttccagtctg accacttcgg
     3961 attatcccgt gacaggtcat tcagactggc taatgcaccc agtaaggcag cggtatcatc
     4021 aacaggctta cccgtcttac tgtccctagt actagtgctt ggattctcac caataaaaaa
     4081 cgcccggcgg caaccgagcg ttctgaacaa atccagatgg agttctgagg tcattactgg
     4141 atctatcaac aggagtccaa gcgagctcgt aaacttggtc tgacagttac caatgcttaa
     4201 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     4261 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     4321 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     4381 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     4441 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     4501 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     4561 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     4621 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     4681 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     4741 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     4801 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     4861 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     4921 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     4981 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     5041 tactcatact cttccttttc aatattattg aagcatttat cagggttatt gtctcatgag
     5101 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     5161 ccgaaaagtg ccacctgacg tctgtggctt ttatgaaaat cacacagtga tcacaaattt
     5221 taaacagagc acaaaatgct gcctcgaaat gagggcggga aaataaggtt atcagccttg
     5281 ttttctccct cattacttga aggatatgaa gctaaaaccc ttttttataa agcatttgtc
     5341 cgaattcgga cataatcaaa aaagcttaat taagatcaat ttgatctaca tctctttaac
     5401 caacaatatg taagatctca actacggaaa aactcatctt tgcctcactg tcaatttgac
     5461 tatagatatt gtcatatcga ccatttgatt gatagtcatt ttgactactc attaatgggc
     5521 ataattttat ttatagagta aaaacaatca gataaaaaac tggcacgcaa tctgcaatta
     5581 gcaagacatc tttttagaac acgattaaag aggagaaggt accatgagag ccctggtagt
     5641 catccgcctg tcccgcgtca ccgatgctac gacttcaccg gagcgtcagc tggagtcttg
     5701 ccagcagctc tgcgcccagc gcggctggga cgtcgtcggg gtagcggagg atctggacgt
     5761 ctccggggcg gtcgatccgt tcgaccggaa gcgcagaccg aacctggccc ggtggctagc
     5821 gttcgaggag caaccgttcg acgtgatcgt ggcgtaccgg gtagaccggt tgacccgatc
     5881 gatccggcat ctgcagcagc tggtccactg ggccgaggac cacaagaagc tggtcgtctc
     5941 cgcgaccgaa gcgcacttcg atacgacgac gccgtttgcg gcggtcgtca tcgcgcttat
     6001 gggaacggtg gcgcagatgg aattagaagc gatcaaagag cggaaccgtt cggctgcgca
     6061 tttcaatatc cgcgccggga aataccgagg atccctgccg ccgtggggat acctgcctac
     6121 gcgcgtggac ggggagtggc ggctggtgcc ggaccctgtg cagcgagagc gcatcctcga
     6181 ggtgtatcac cgcgtcgtcg acaaccacga gccgctgcac ctggtggccc acgacctgaa
     6241 ccggcgtggt gtcctgtcgc cgaaggacta cttcgcgcag ctgcaaggcc gcgagccgca
     6301 gggccgggag tggtcggcta ccgcgctgaa gcgatcgatg atctccgagg cgatgctcgg
     6361 gtacgcgact ctgaacggta agaccgtccg agacgacgac ggagccccgc tggtgcgggc
     6421 tgagccgatc ctgacccgtg agcagctgga ggcgctgcgc gccgagctcg tgaagacctc
     6481 ccgggcgaag cccgcggtgt ctaccccgtc gctgctgctg cgggtgttgt tctgtgcggt
     6541 gtgcggggag cccgcgtaca agttcgccgg gggaggacgt aagcacccgc gctaccgctg
     6601 ccgctcgatg gggttcccga agcactgcgg gaacggcacg gtggcgatgg ccgagtggga
     6661 cgcgttctgc gaggagcagg tgctggatct gctcggggac gcggagcgtc tggagaaagt
     6721 ctgggtagcc ggctcggact ccgcggtcga actcgcggag gtgaacgcgg agctggtgga
     6781 cctgacgtcg ctgatcggct ccccggccta ccgggccggc tctccgcagc gagaagcact
     6841 ggatgcccgt attgcggcgc tggccgcgcg gcaagaggag ctggagggtc tagaggctcg
     6901 cccgtctggc tgggagtggc gcgagaccgg gcagcggttc ggggactggt ggcgggagca
     6961 ggacaccgcg gcaaagaaca cctggcttcg gtcgatgaac gttcggctga cgttcgacgt
     7021 ccgcggcggg ctgactcgca cgatcgactt cggggatctg caggagtacg agcagcatct
     7081 caggctcggc agcgtggtcg aacggctaca caccgggatg tcgaggcctg cagcaaacga
     7141 cgaaaactac gctgcagcag tttagacaca tggcatggat gaactataca aataacccgg
     7201 gggatcccat ggtacgcgtg ctagaggcat caaataaaac gaaaggctca gtcgaaagac
     7261 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg
     7321 ccgccctaga cctag
//
