LOCUS       sequence-391272-        7874 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Synthetic gene circuit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7874)
  AUTHORS   Inda-Webb ME, Jimenez M, Liu Q, Phan NV, Ahn J, Steiger C, Wentworth
            A, Riaz A, Zirtiloglu T, Wong K, Ishida K, Fabian N, Jenkins J, 
            Kuosmanen J, Madani W, McNally R, Lai Y, Hayward A, Mimee M, Nadeau 
            P, Chandrakasan AP, Traverso G, Yazicigil RT, Lu TK
  TITLE     Sub-1.4 cm(3) capsule for detecting labile inflammatory biomarkers 
            in situ.
  JOURNAL   Nature. 2023 Aug;620(7973):386-392. doi: 10.1038/s41586-023-06369-x.
            Epub 2023 Jul 26.
  PUBMED    37495692
REFERENCE   2  (bases 1 to 7874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-023-06369-x"; journalName: "Nature"; date: 
            "2023-08"; volume: "620"; issue: "7973"; pages: "386-392"
COMMENT     Sequence Label: TT MS-Sensor
FEATURES             Location/Qualifiers
     source          1..7874
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1125..1211
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(1756..2706)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      2754..2976
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(3474..3568)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(3598..4389,4390..4458))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4221..4240
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4459..4562)
                     /gene="bla"
                     /label=AmpR promoter
     RBS             5017..5028
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     primer_bind     5078..5096
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5117..5151
                     /label=BBa_J23109
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.04"
     terminator      5822..5893
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5909..5936
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     RBS             6145..6156
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             6163..7662
                     /codon_start=1
                     /transl_table=11
                     /gene="Bxb1"
                     /product="Bxb1 integrase"
                     /label=Bxb1 integrase
                     /note="Bxb1 large serine integrase from Mycobacterium 
                     bacteriophage Bxb1 (Ghosh et al. 2003)."
                     /protein_id="AFF61396.1"
                     /translation="MRALVVIRLSRVTDATTSPERQLESCQQLCAQRGWDVVGVAEDLD
                     VSGAVDPFDRKRRPNLARWLAFEEQPFDVIVAYRVDRLTRSIRHLQQLVHWAEDHKKLV
                     VSATEAHFDTTTPFAAVVIALMGTVAQMELEAIKERNRSAAHFNIRAGKYRGSLPPWGY
                     LPTRVDGEWRLVPDPVQRERILEVYHRVVDNHEPLHLVAHDLNRRGVLSPKDYFAQLQG
                     REPQGREWSATALKRSMISEAMLGYATLNGKTVRDDDGAPLVRAEPILTREQLEALRAE
                     LVKTSRAKPAVSTPSLLLRVLFCAVCGEPAYKFAGGGRKHPRYRCRSMGFPKHCGNGTV
                     AMAEWDAFCEEQVLDLLGDAERLEKVWVAGSDSAVELAEVNAELVDLTSLIGSPAYRAG
                     SPQREALDARIAALAARQEELEGLEARPSGWEWRETGQRFGDWWREQDTAAKNTWLRSM
                     NVRLTFDVRGGLTRTIDFGDLQEYEQHLRLGSVVERLHTGMS"
     terminator      7770..7856
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cacagctaac accacgtcgt ccctatctgc tgccctaggt ctatgagtgg ttgctggata
       61 actttacggg catgcataag gctcgtataa tatattcagg gagaccacaa cggtttccct
      121 ctacaaataa ttttgtttaa ctttgaattc ttcacacagg aaaccggtac catgaatatt
      181 cgtgatcttg agtacctggt ggcattggct gaacaccgcc attttcggcg tgcggcagat
      241 tcctgccacg ttagccagcc gacgcttagc gggcaaattc gtaagctgga agatgagctg
      301 ggcgtgatgt tgctggagcg gaccagccgt aaagtgttgt tcacccaggc gggaatgctg
      361 ctggtggatc aggcgcgtac cgtgctgcgt gaggtgaaag tccttaaaga gatggcaagc
      421 cagcagggcg agacgatgtc cggaccgctg cacattggtt tgattcccac agttggaccg
      481 tacctgctac cgcatattat ccctatgctg caccagacct ttccaaagct ggaaatgtat
      541 ctgcatgaag cacagaccca ccagttactg gcgcaactgg acagcggcaa actcgattgc
      601 gtgatcctcg cgctggtgaa agagagcgaa gcattcattg aagtgccgtt gtttgatgag
      661 ccaatgttgc tggctatcta tgaagatcac ccgtgggcga accgcgaatg cgtaccgatg
      721 gccgatctgg caggggaaaa actgctgatg ctggaagatg gtcactgttt gcgcgatcag
      781 gcaatgggtt tctgttttga agccggggcg gatgaagata cacacttccg cgcgaccagc
      841 ctggaaactc tgcgcaacat ggtggcggca ggtagcggga tcactttact gccagcgctg
      901 gctgtgccgc cggagcgcaa acgcgatggg gttgtttatc tgccgtgcat taagccggaa
      961 ccacgccgca ctattggcct ggtttatcgt cctggctcac cgctgcgcag ccgctatgag
     1021 cagctggcag aggccatccg cgcaagaatg gatggccatt tcgataaagt tttaaaacag
     1081 gcggtttaac ccgggggatc ccatggtacg cgtgctagag gcatcaaata aaacgaaagg
     1141 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga
     1201 gtaggacaaa tccgccgccc tagacctagg gcctagggta cgggttttgc tgcccgcaaa
     1261 cgggctgttc tggtgttgct agtttgttat cagaatcgca gatccggctt cagccggttt
     1321 gccggctgaa agcgctattt cttccagaat tgccatgatt ttttccccac gggaggcgtc
     1381 actggctccc gtgttgtcgg cagctttgat tcgataagca gcatcgcctg tttcaggctg
     1441 tctatgtgtg actgttgagc tgtaacaagt tgtctcaggt gttcaatttc atgttctagt
     1501 tgctttgttt tactggtttc acctgttcta ttaggtgtta catgctgttc atctgttaca
     1561 ttgtcgatct gttcatggtg aacagctttg aatgcaccaa aaactcgtaa aagctctgat
     1621 gtatctatct tttttacacc gttttcatct gtgcatatgg acagttttcc ctttgatatg
     1681 taacggtgaa cagttgttct acttttgttt gttagtcttg atgcttcact gatagataca
     1741 agagccataa gaacctcaga tccttccgta tttagccagt atgttctcta gtgtggttcg
     1801 ttgtttttgc gtgagccatg agaacgaacc attgagatca tacttacttt gcatgtcact
     1861 caaaaatttt gcctcaaaac tggtgagctg aatttttgca gttaaagcat cgtgtagtgt
     1921 ttttcttagt ccgttatgta ggtaggaatc tgatgtaatg gttgttggta ttttgtcacc
     1981 attcattttt atctggttgt tctcaagttc ggttacgaga tccatttgtc tatctagttc
     2041 aacttggaaa atcaacgtat cagtcgggcg gcctcgctta tcaaccacca atttcatatt
     2101 gctgtaagtg tttaaatctt tacttattgg tttcaaaacc cattggttaa gccttttaaa
     2161 ctcatggtag ttattttcaa gcattaacat gaacttaaat tcatcaaggc taatctctat
     2221 atttgccttg tgagttttct tttgtgttag ttcttttaat aaccactcat aaatcctcat
     2281 agagtatttg ttttcaaaag acttaacatg ttccagatta tattttatga atttttttaa
     2341 ctggaaaaga taaggcaata tctcttcact aaaaactaat tctaattttt cgcttgagaa
     2401 cttggcatag tttgtccact ggaaaatctc aaagccttta accaaaggat tcctgatttc
     2461 cacagttctc gtcatcagct ctctggttgc tttagctaat acaccataag cattttccct
     2521 actgatgttc atcatctgag cgtattggtt ataagtgaac gataccgtcc gttctttcct
     2581 tgtagggttt tcaatcgtgg ggttgagtag tgccacacag cataaaatta gcttggtttc
     2641 atgctccgtt aagtcatagc gactaatcgc tagttcattt gctttgaaaa caactaattc
     2701 agacatacat ctcaattggt ctaggtgatt ttaatcacta taccaattga gatgggctag
     2761 tcaatgataa ttactagtcc ttttcctttg agttgtgggt atctgtaaat tctgctagac
     2821 ctttgctgga aaacttgtaa attctgctag accctctgta aattccgcta gacctttgtg
     2881 tgtttttttt gtttatattc aagtggttat aatttataga ataaagaaag aataaaaaaa
     2941 gataaaaaga atagatccca gccctgtgta taactcacta ctttagtcag ttccgcagta
     3001 ttacaaaagg atgtcgcaaa cgctgtttgc tcctctacaa aacagacctt aaaaccctaa
     3061 aggcttaagt agcaccctcg caagctcggg caaatcgctg aatattcctt ttgtctccga
     3121 ccatcaggca cctgagtcgc tgtctttttc gtgacattca gttcgctgcg ctcacggctc
     3181 tggcagtgaa tgggggtaaa tggcactaca ggcgcctttt atggattcat gcaaggaaac
     3241 tacccataat acaagaaaag cccgtcacgg gcttctcagg gcgttttatg gcgggtctgc
     3301 tatgtggtgc tatctgactt tttgctgttc agcagttcct gccctctgat tttccagtct
     3361 gaccacttcg gattatcccg tgacaggtca ttcagactgg ctaatgcacc cagtaaggca
     3421 gcggtatcat caacaggctt acccgtctta ctgtccctag tactagtgct tggattctca
     3481 ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg gagttctgag
     3541 gtcattactg gatctatcaa caggagtcca agcgagctcg taaacttggt ctgacagtta
     3601 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     3661 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     3721 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     3781 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     3841 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     3901 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     3961 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     4021 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     4081 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     4141 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     4201 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     4261 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     4321 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     4381 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     4441 gaaatgttga atactcatac tcttcctttt caatattatt gaagcattta tcagggttat
     4501 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     4561 cgcacatttc cccgaaaagt gccacctgac gtctgtggct tttatgaaaa tcacacagtg
     4621 atcacaaatt ttaaacagag cacaaaatgc tgcctcgaaa tgagggcggg aaaataaggt
     4681 tatcagcctt gttttctccc tcattacttg aaggatatga agctaaaacc cttttttata
     4741 aagcatttgt ccgaattcgg acataatcaa aaaagcttaa ttaagatcaa tttgatctac
     4801 atctctttaa ccaacaatat gtaagatctc aactacggaa aaactcatct ttgcctcact
     4861 gtcaatttga ctatagatat tgtcatatcg accatttgat tgatagtcat tttgactact
     4921 cattaatggg cataatttta tttatagagt aaaaacaatc agataaaaaa ctggcacgca
     4981 atctgcaatt agcaagacat ctttttagaa cacgctaaag aggagaaagg taccatgacg
     5041 gaaaaactca tctttgcctc actgtcaatt tgactataat aggcgtatca cgaggccctt
     5101 tcgtcttcac ctcgagttta cagctagctc agtcctaggg actgtgctag caccaaatat
     5161 agagaatcac aaggatctat tcaatgagcc tggcgctgcc ggtgtacctg attgacgacg
     5221 atgactccgt tcgccgttcc ctgcgtttca tgctggaaag ctacggcctg aaaatcactg
     5281 acttcgattc tgctgaagcg ttcttcaccg cggtagacct gaccctgccg ggttgcgcac
     5341 tggtggacgt acgtatgccg ggcctgagcg gcccacagct gcacctggaa ctggtgtcta
     5401 agaacagccc gctggccgtg atctatctga ccggtcacgg cgacgttccg atggcggttg
     5461 aagcgctgaa actgggtgcg gtagatttct ttcagaaacc tgcagacggc gctaaactgg
     5521 ctgaagctgt ggtcaaagcc ctggaacacg cgaaaaccca ctaccaagac aaccagtacc
     5581 tggaaaccta tcaggctctg accccacgtg agcgcgaaat cctgaacctg attgcgcaag
     5641 gtctgaaaaa ccaggaaatc gcggacaacc tgtgcattgc gatgcgcacc gtagaaatcc
     5701 accgtgcgaa cctgatgaaa ggtatgcagg tgggcagcct ggcagagatg atgctgattt
     5761 actctcgtat cgcggagcgt ctgcgcctgg aagataaccg ttaataatct agaccaggca
     5821 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
     5881 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta
     5941 tagtaaatca ctgcataatt cgtgtcgctc aaggcgcact cccgttctgg ataatgtttt
     6001 ttgcgccgac atcataacgg ttctggcaaa tattctgaaa tgagctggtt agctagtcaa
     6061 tatttgttgc gctagatcaa atccacgcct gatatgtgga aaaccactat agttatgccg
     6121 cctcgccttt tacagaatgc ctgtaaagag gagaaaggta ccatgagagc cctggtagtc
     6181 atccgcctgt cccgcgtcac cgatgctacg acttcaccgg agcgtcagct ggagtcttgc
     6241 cagcagctct gcgcccagcg cggctgggac gtcgtcgggg tagcggagga tctggacgtc
     6301 tccggggcgg tcgatccgtt cgaccggaag cgcagaccga acctggcccg gtggctagcg
     6361 ttcgaggagc aaccgttcga cgtgatcgtg gcgtaccggg tagaccggtt gacccgatcg
     6421 atccggcatc tgcagcagct ggtccactgg gccgaggacc acaagaagct ggtcgtctcc
     6481 gcgaccgaag cgcacttcga tacgacgacg ccgtttgcgg cggtcgtcat cgcgcttatg
     6541 ggaacggtgg cgcagatgga attagaagcg atcaaagagc ggaaccgttc ggctgcgcat
     6601 ttcaatatcc gcgccgggaa ataccgagga tccctgccgc cgtggggata cctgcctacg
     6661 cgcgtggacg gggagtggcg gctggtgccg gaccctgtgc agcgagagcg catcctcgag
     6721 gtgtatcacc gcgtcgtcga caaccacgag ccgctgcacc tggtggccca cgacctgaac
     6781 cggcgtggtg tcctgtcgcc gaaggactac ttcgcgcagc tgcaaggccg cgagccgcag
     6841 ggccgggagt ggtcggctac cgcgctgaag cgatcgatga tctccgaggc gatgctcggg
     6901 tacgcgactc tgaacggtaa gaccgtccga gacgacgacg gagccccgct ggtgcgggct
     6961 gagccgatcc tgacccgtga gcagctggag gcgctgcgcg ccgagctcgt gaagacctcc
     7021 cgggcgaagc ccgcggtgtc taccccgtcg ctgctgctgc gggtgttgtt ctgtgcggtg
     7081 tgcggggagc ccgcgtacaa gttcgccggg ggaggacgta agcacccgcg ctaccgctgc
     7141 cgctcgatgg ggttcccgaa gcactgcggg aacggcacgg tggcgatggc cgagtgggac
     7201 gcgttctgcg aggagcaggt gctggatctg ctcggggacg cggagcgtct ggagaaagtc
     7261 tgggtagccg gctcggactc cgcggtcgaa ctcgcggagg tgaacgcgga gctggtggac
     7321 ctgacgtcgc tgatcggctc cccggcctac cgggccggct ctccgcagcg agaagcactg
     7381 gatgcccgta ttgcggcgct ggccgcgcgg caagaggagc tggagggtct agaggctcgc
     7441 ccgtctggct gggagtggcg cgagaccggg cagcggttcg gggactggtg gcgggagcag
     7501 gacaccgcgg caaagaacac ctggcttcgg tcgatgaacg ttcggctgac gttcgacgtc
     7561 cgcggcgggc tgactcgcac gatcgacttc ggggatctgc aggagtacga gcagcatctc
     7621 aggctcggca gcgtggtcga acggctacac accgggatgt cgaggcctgc agcaaacgac
     7681 gaaaactacg ctgcagcagt ttagacacat ggcatggatg aactatacaa ataacccggg
     7741 ggatcccatg gtacgcgtgc tagaggcatc aaataaaacg aaaggctcag tcgaaagact
     7801 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc
     7861 cgccctagac ctag
//
