LOCUS       sequence-391281-        8366 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Synthetic gene circuit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8366)
  AUTHORS   Inda-Webb ME, Jimenez M, Liu Q, Phan NV, Ahn J, Steiger C, Wentworth
            A, Riaz A, Zirtiloglu T, Wong K, Ishida K, Fabian N, Jenkins J, 
            Kuosmanen J, Madani W, McNally R, Lai Y, Hayward A, Mimee M, Nadeau 
            P, Chandrakasan AP, Traverso G, Yazicigil RT, Lu TK
  TITLE     Sub-1.4 cm(3) capsule for detecting labile inflammatory biomarkers 
            in situ.
  JOURNAL   Nature. 2023 Aug;620(7973):386-392. doi: 10.1038/s41586-023-06369-x.
            Epub 2023 Jul 26.
  PUBMED    37495692
REFERENCE   2  (bases 1 to 8366)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-023-06369-x"; journalName: "Nature"; date: 
            "2023-08"; volume: "620"; issue: "7973"; pages: "386-392"
COMMENT     Sequence Label: TS MS-Sensor
FEATURES             Location/Qualifiers
     source          1..8366
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1125..1211
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(1756..2706)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      2754..2976
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(3820..3914)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(3944..4735,4736..4804))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4567..4586
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4805..4908)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5320..5338
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5359..5393
                     /label=BBa_J23105
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.24"
     terminator      6057..6128
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      6144..6171
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     RBS             6637..6648
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             6655..8154
                     /codon_start=1
                     /transl_table=11
                     /gene="Bxb1"
                     /product="Bxb1 integrase"
                     /label=Bxb1 integrase
                     /note="Bxb1 large serine integrase from Mycobacterium 
                     bacteriophage Bxb1 (Ghosh et al. 2003)."
                     /protein_id="AFF61396.1"
                     /translation="MRALVVIRLSRVTDATTSPERQLESCQQLCAQRGWDVVGVAEDLD
                     VSGAVDPFDRKRRPNLARWLAFEEQPFDVIVAYRVDRLTRSIRHLQQLVHWAEDHKKLV
                     VSATEAHFDTTTPFAAVVIALMGTVAQMELEAIKERNRSAAHFNIRAGKYRGSLPPWGY
                     LPTRVDGEWRLVPDPVQRERILEVYHRVVDNHEPLHLVAHDLNRRGVLSPKDYFAQLQG
                     REPQGREWSATALKRSMISEAMLGYATLNGKTVRDDDGAPLVRAEPILTREQLEALRAE
                     LVKTSRAKPAVSTPSLLLRVLFCAVCGEPAYKFAGGGRKHPRYRCRSMGFPKHCGNGTV
                     AMAEWDAFCEEQVLDLLGDAERLEKVWVAGSDSAVELAEVNAELVDLTSLIGSPAYRAG
                     SPQREALDARIAALAARQEELEGLEARPSGWEWRETGQRFGDWWREQDTAAKNTWLRSM
                     NVRLTFDVRGGLTRTIDFGDLQEYEQHLRLGSVVERLHTGMS"
     terminator      8262..8348
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cacagctaac accacgtcgt ccctatctgc tgccctaggt ctatgagtgg ttgctggata
       61 actttacggg catgcataag gctcgtataa tatattcagg gagaccacaa cggtttccct
      121 ctacaaataa ttttgtttaa ctttgaattc ttcacacagg aaaccggtac catgaatatt
      181 cgtgatcttg agtacctggt ggcattggct gaacaccgcc attttcggcg tgcggcagat
      241 tcctgccacg ttagccagcc gacgcttagc gggcaaattc gtaagctgga agatgagctg
      301 ggcgtgatgt tgctggagcg gaccagccgt aaagtgttgt tcacccaggc gggaatgctg
      361 ctggtggatc aggcgcgtac cgtgctgcgt gaggtgaaag tccttaaaga gatggcaagc
      421 cagcagggcg agacgatgtc cggaccgctg cacattggtt tgattcccac agttggaccg
      481 tacctgctac cgcatattat ccctatgctg caccagacct ttccaaagct ggaaatgtat
      541 ctgcatgaag cacagaccca ccagttactg gcgcaactgg acagcggcaa actcgattgc
      601 gtgatcctcg cgctggtgaa agagagcgaa gcattcattg aagtgccgtt gtttgatgag
      661 ccaatgttgc tggctatcta tgaagatcac ccgtgggcga accgcgaatg cgtaccgatg
      721 gccgatctgg caggggaaaa actgctgatg ctggaagatg gtcactgttt gcgcgatcag
      781 gcaatgggtt tctgttttga agccggggcg gatgaagata cacacttccg cgcgaccagc
      841 ctggaaactc tgcgcaacat ggtggcggca ggtagcggga tcactttact gccagcgctg
      901 gctgtgccgc cggagcgcaa acgcgatggg gttgtttatc tgccgtgcat taagccggaa
      961 ccacgccgca ctattggcct ggtttatcgt cctggctcac cgctgcgcag ccgctatgag
     1021 cagctggcag aggccatccg cgcaagaatg gatggccatt tcgataaagt tttaaaacag
     1081 gcggtttaac ccgggggatc ccatggtacg cgtgctagag gcatcaaata aaacgaaagg
     1141 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga
     1201 gtaggacaaa tccgccgccc tagacctagg gcctagggta cgggttttgc tgcccgcaaa
     1261 cgggctgttc tggtgttgct agtttgttat cagaatcgca gatccggctt cagccggttt
     1321 gccggctgaa agcgctattt cttccagaat tgccatgatt ttttccccac gggaggcgtc
     1381 actggctccc gtgttgtcgg cagctttgat tcgataagca gcatcgcctg tttcaggctg
     1441 tctatgtgtg actgttgagc tgtaacaagt tgtctcaggt gttcaatttc atgttctagt
     1501 tgctttgttt tactggtttc acctgttcta ttaggtgtta catgctgttc atctgttaca
     1561 ttgtcgatct gttcatggtg aacagctttg aatgcaccaa aaactcgtaa aagctctgat
     1621 gtatctatct tttttacacc gttttcatct gtgcatatgg acagttttcc ctttgatatg
     1681 taacggtgaa cagttgttct acttttgttt gttagtcttg atgcttcact gatagataca
     1741 agagccataa gaacctcaga tccttccgta tttagccagt atgttctcta gtgtggttcg
     1801 ttgtttttgc gtgagccatg agaacgaacc attgagatca tacttacttt gcatgtcact
     1861 caaaaatttt gcctcaaaac tggtgagctg aatttttgca gttaaagcat cgtgtagtgt
     1921 ttttcttagt ccgttatgta ggtaggaatc tgatgtaatg gttgttggta ttttgtcacc
     1981 attcattttt atctggttgt tctcaagttc ggttacgaga tccatttgtc tatctagttc
     2041 aacttggaaa atcaacgtat cagtcgggcg gcctcgctta tcaaccacca atttcatatt
     2101 gctgtaagtg tttaaatctt tacttattgg tttcaaaacc cattggttaa gccttttaaa
     2161 ctcatggtag ttattttcaa gcattaacat gaacttaaat tcatcaaggc taatctctat
     2221 atttgccttg tgagttttct tttgtgttag ttcttttaat aaccactcat aaatcctcat
     2281 agagtatttg ttttcaaaag acttaacatg ttccagatta tattttatga atttttttaa
     2341 ctggaaaaga taaggcaata tctcttcact aaaaactaat tctaattttt cgcttgagaa
     2401 cttggcatag tttgtccact ggaaaatctc aaagccttta accaaaggat tcctgatttc
     2461 cacagttctc gtcatcagct ctctggttgc tttagctaat acaccataag cattttccct
     2521 actgatgttc atcatctgag cgtattggtt ataagtgaac gataccgtcc gttctttcct
     2581 tgtagggttt tcaatcgtgg ggttgagtag tgccacacag cataaaatta gcttggtttc
     2641 atgctccgtt aagtcatagc gactaatcgc tagttcattt gctttgaaaa caactaattc
     2701 agacatacat ctcaattggt ctaggtgatt ttaatcacta taccaattga gatgggctag
     2761 tcaatgataa ttactagtcc ttttcctttg agttgtgggt atctgtaaat tctgctagac
     2821 ctttgctgga aaacttgtaa attctgctag accctctgta aattccgcta gacctttgtg
     2881 tgtttttttt gtttatattc aagtggttat aatttataga ataaagaaag aataaaaaaa
     2941 gataaaaaga atagatccca gccctgtgta taactcacta ctttagtcag ttccgcagta
     3001 ttacaaaagg atgtcgcaaa cgctgtttgc tcctctacaa aacagacctt aaaaccctaa
     3061 aggcttaagt agcaccctcg caagctcggg caaatcgctg aatattcctt ttgtctccga
     3121 ccatcaggca cctgagtcgc tgtctttttc gtgacattca gttcgctgcg ctcacggctc
     3181 tggcagtgaa tgggggtaaa tggcactaca ggcgcctttt atggattcat gcaaggaaac
     3241 tacccataat acaagaaaag cccgtcacgg gcttctcagg gcgttttatg gcgggtctgc
     3301 tatgtggtgc tatctgactt tttgctgttc agcagttcct gccctctgat tttccagtct
     3361 gaccacttcg gattatcccg tgacaggtca gaccttaaaa ccctaaaggc ttaagtagca
     3421 ccctcgcaag ctcgggcaaa tcgctgaata ttccttttgt ctccgaccat caggcacctg
     3481 agtcgctgtc tttttcgtga cattcagttc gctgcgctca cggctctggc agtgaatggg
     3541 ggtaaatggc actacaggcg ccttttatgg attcatgcaa ggaaactacc cataatacaa
     3601 gaaaagcccg tcacgggctt ctcagggcgt tttatggcgg gtctgctatg tggtgctatc
     3661 tgactttttg ctgttcagca gttcctgccc tctgattttc cagtctgacc acttcggatt
     3721 atcccgtgac aggtcattca gactggctaa tgcacccagt aaggcagcgg tatcatcaac
     3781 aggcttaccc gtcttactgt ccctagtact agtgcttgga ttctcaccaa taaaaaacgc
     3841 ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca ttactggatc
     3901 tatcaacagg agtccaagcg agctcgtaaa cttggtctga cagttaccaa tgcttaatca
     3961 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     4021 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     4081 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     4141 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     4201 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     4261 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     4321 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     4381 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     4441 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     4501 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     4561 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     4621 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     4681 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     4741 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     4801 tcatactctt ccttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     4861 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     4921 aaaagtgcca cctgacgtct gtggctttta tgaaaatcac acagtgatca caaattttaa
     4981 acagagcaca aaatgctgcc tcgaaatgag ggcgggaaaa taaggttatc agccttgttt
     5041 tctccctcat tacttgaagg atatgaagct aaaacccttt tttataaagc atttgtccga
     5101 attcggacat aatcaaaaaa gcttaattaa gatcaatttg atctacatct ctttaaccaa
     5161 caatatgtaa gatctcaact acggaaaaac tcatctttgc ctcacgtcaa tttgactatg
     5221 ctttctaact aaaaaggcct cccaaatcgg ggggcctttt ttattgataa caaaacgctc
     5281 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     5341 tttcgtcttc acctcgagtt tacggctagc tcagtcctag gtactatgct agcagaaata
     5401 taaagaacga tctatttatc cgcgtacatg cagcagcaaa tcaacggccc ggtctacctg
     5461 gtggatgatg atgaagccat tatcgactcc atcgattttt tgatggaggg ctacggttac
     5521 aaactgaact cgtttaactg cggcgatcgc tttttggcag aagtcgatct cacccaggca
     5581 ggatgtgtaa ttctggatgc gcgtatgcca ggcttaactg gtcctcaggt gcaacagctg
     5641 ctgagcgacg cgaaaagccc gcttgcggtc atcttcctga ccggccatgg cgatgttccg
     5701 atggcggttg atgcgttcaa aaatggcgcg ttcgatttct ttcaaaaacc tgtgccgggt
     5761 agcttgctca gtcagtcaat tgccaaaggc ttgacttatt caatcgatca acatctgaaa
     5821 cgtactaacc aagcgttaat cgacacgctc tcggaacgcg aagctcaaat ttttcaactg
     5881 gtgattgcag gcaacaccaa caaacagatg gctaacgagc tttgcgtggc tattcgtacc
     5941 attgaggttc accgtagcaa actgatgacc aaactgggtg ttaacaacct ggctgaactg
     6001 gttaaactgg cgccgctgct ggcacataaa tccgaataat aatctagacc aggcatcaaa
     6061 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     6121 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatagta
     6181 aatcactgca taattcgtgt cgctcaaggc gcactcccgt tctggataat gttttttgcg
     6241 ccgacatcat aacggttctg gcaaatattc tgaaatgagc tggttagcta gtcattcaag
     6301 cattattatg ctgttttttg aagtgaatgt gcggccatct agccgcacat tttgcatcta
     6361 aaacatgcag tcatcagcaa aataataaac ttttccccaa tatgtggttt accacaattt
     6421 acaggaattc actcctgtgg tggtgcaaat ttgaactgtg aattgcttca caaacgccgc
     6481 tatcgcaatg tcagtatgtg gtttaccaca atatctaata tcactctgct caataacaat
     6541 gatgaaaacc ttaggaagaa gttaattgtg ttaaacagtt aactaggggc tttatctaac
     6601 gctctcctaa ggacaactgt cattgggaga tttaacaaag aggagaaagg taccatgaga
     6661 gccctggtag tcatccgcct gtcccgcgtc accgatgcta cgacttcacc ggagcgtcag
     6721 ctggagtctt gccagcagct ctgcgcccag cgcggctggg acgtcgtcgg ggtagcggag
     6781 gatctggacg tctccggggc ggtcgatccg ttcgaccgga agcgcagacc gaacctggcc
     6841 cggtggctag cgttcgagga gcaaccgttc gacgtgatcg tggcgtaccg ggtagaccgg
     6901 ttgacccgat cgatccggca tctgcagcag ctggtccact gggccgagga ccacaagaag
     6961 ctggtcgtct ccgcgaccga agcgcacttc gatacgacga cgccgtttgc ggcggtcgtc
     7021 atcgcgctta tgggaacggt ggcgcagatg gaattagaag cgatcaaaga gcggaaccgt
     7081 tcggctgcgc atttcaatat ccgcgccggg aaataccgag gatccctgcc gccgtgggga
     7141 tacctgccta cgcgcgtgga cggggagtgg cggctggtgc cggaccctgt gcagcgagag
     7201 cgcatcctcg aggtgtatca ccgcgtcgtc gacaaccacg agccgctgca cctggtggcc
     7261 cacgacctga accggcgtgg tgtcctgtcg ccgaaggact acttcgcgca gctgcaaggc
     7321 cgcgagccgc agggccggga gtggtcggct accgcgctga agcgatcgat gatctccgag
     7381 gcgatgctcg ggtacgcgac tctgaacggt aagaccgtcc gagacgacga cggagccccg
     7441 ctggtgcggg ctgagccgat cctgacccgt gagcagctgg aggcgctgcg cgccgagctc
     7501 gtgaagacct cccgggcgaa gcccgcggtg tctaccccgt cgctgctgct gcgggtgttg
     7561 ttctgtgcgg tgtgcgggga gcccgcgtac aagttcgccg ggggaggacg taagcacccg
     7621 cgctaccgct gccgctcgat ggggttcccg aagcactgcg ggaacggcac ggtggcgatg
     7681 gccgagtggg acgcgttctg cgaggagcag gtgctggatc tgctcgggga cgcggagcgt
     7741 ctggagaaag tctgggtagc cggctcggac tccgcggtcg aactcgcgga ggtgaacgcg
     7801 gagctggtgg acctgacgtc gctgatcggc tccccggcct accgggccgg ctctccgcag
     7861 cgagaagcac tggatgcccg tattgcggcg ctggccgcgc ggcaagagga gctggagggt
     7921 ctagaggctc gcccgtctgg ctgggagtgg cgcgagaccg ggcagcggtt cggggactgg
     7981 tggcgggagc aggacaccgc ggcaaagaac acctggcttc ggtcgatgaa cgttcggctg
     8041 acgttcgacg tccgcggcgg gctgactcgc acgatcgact tcggggatct gcaggagtac
     8101 gagcagcatc tcaggctcgg cagcgtggtc gaacggctac acaccgggat gtcgaggcct
     8161 gcagcaaacg acgaaaacta cgctgcagca gtttagacac atggcatgga tgaactatac
     8221 aaataacccg ggggatccca tggtacgcgt gctagaggca tcaaataaaa cgaaaggctc
     8281 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta
     8341 ggacaaatcc gccgccctag acctag
//
