LOCUS       sequence-391283-        7681 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Synthetic gene circuit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7681)
  AUTHORS   Inda-Webb ME, Jimenez M, Liu Q, Phan NV, Ahn J, Steiger C, Wentworth
            A, Riaz A, Zirtiloglu T, Wong K, Ishida K, Fabian N, Jenkins J, 
            Kuosmanen J, Madani W, McNally R, Lai Y, Hayward A, Mimee M, Nadeau 
            P, Chandrakasan AP, Traverso G, Yazicigil RT, Lu TK
  TITLE     Sub-1.4 cm(3) capsule for detecting labile inflammatory biomarkers 
            in situ.
  JOURNAL   Nature. 2023 Aug;620(7973):386-392. doi: 10.1038/s41586-023-06369-x.
            Epub 2023 Jul 26.
  PUBMED    37495692
REFERENCE   2  (bases 1 to 7681)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-023-06369-x"; journalName: "Nature"; date: 
            "2023-08"; volume: "620"; issue: "7973"; pages: "386-392"
COMMENT     Sequence Label: NO- DSD Sensor 2
FEATURES             Location/Qualifiers
     source          1..7681
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1714..1800
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(2345..3295)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      3343..3565
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(4409..4503)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(4533..5324,5325..5393))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5156..5175
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5394..5497)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5970..7469
                     /codon_start=1
                     /transl_table=11
                     /gene="Bxb1"
                     /product="Bxb1 integrase"
                     /label=Bxb1 integrase
                     /note="Bxb1 large serine integrase from Mycobacterium 
                     bacteriophage Bxb1 (Ghosh et al. 2003)."
                     /protein_id="AFF61396.1"
                     /translation="MRALVVIRLSRVTDATTSPERQLESCQQLCAQRGWDVVGVAEDLD
                     VSGAVDPFDRKRRPNLARWLAFEEQPFDVIVAYRVDRLTRSIRHLQQLVHWAEDHKKLV
                     VSATEAHFDTTTPFAAVVIALMGTVAQMELEAIKERNRSAAHFNIRAGKYRGSLPPWGY
                     LPTRVDGEWRLVPDPVQRERILEVYHRVVDNHEPLHLVAHDLNRRGVLSPKDYFAQLQG
                     REPQGREWSATALKRSMISEAMLGYATLNGKTVRDDDGAPLVRAEPILTREQLEALRAE
                     LVKTSRAKPAVSTPSLLLRVLFCAVCGEPAYKFAGGGRKHPRYRCRSMGFPKHCGNGTV
                     AMAEWDAFCEEQVLDLLGDAERLEKVWVAGSDSAVELAEVNAELVDLTSLIGSPAYRAG
                     SPQREALDARIAALAARQEELEGLEARPSGWEWRETGQRFGDWWREQDTAAKNTWLRSM
                     NVRLTFDVRGGLTRTIDFGDLQEYEQHLRLGSVVERLHTGMS"
     terminator      7577..7663
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cacagctaac accacgtcgt ccctatctgc tgccctaggt ctatgagtgg ttgctggata
       61 actttacggg catgcataag gctcgtataa tatattcagg gagaccacaa cggtttccct
      121 ctacaaataa ttttgtttaa ctttgaattc ttcacacagg aaaccggtac catgagtttt
      181 tccgttgatg tgctggcgaa tatcgccatc gaattgcagc gtgggattgg tcaccaggat
      241 cgttttcagc gcctgatcac cacgctacgt caggtgctgg agtgcgatgc gtctgcgttg
      301 ctacgttacg attcgcggca gtttattccg cttgccatcg acggtctggc aaaggatgta
      361 ctcggtagac gctttgcgct ggaagggcat ccacggctgg aagcgattgc ccgcgccggg
      421 gatgtggtgc gctttcccgc agacagcgaa ttgcccgatc cctatgacgg tttgattcct
      481 gggcaggaga gtctgaaggt tcacgcctgc gttggtctgc cattgtttgc cgggcaaaac
      541 ctgatcggcg cactgacgct cgacgggatg cagcccgatc agttcgatgt tttcagcgac
      601 gaagagctac ggctgattgc tgcgctggcg gcgggagcgt taagcaatgc gttgctgatt
      661 gaacaactgg aaagccagaa tatgctgcca ggcgatgcca cgccgtttga agcggtgaaa
      721 cagacgcaga tgattggctt gtcccctggc atgacgcaac tgaaaaaaga gattgagatt
      781 gtggcggcgt ccgatctcaa cgtcctgatc agcggtgaga ctggaaccgg taaggagctg
      841 gtggcgaaag cgattcatga agcctcgcca cgggcggtga atccgctggt ctatctcaac
      901 tgtgctgcac tgccggaaag tgtggcggaa agtgagttgt tcgggcatgt gaaaggagcg
      961 tttactggcg ctatcagtaa tcgcagcggg aagtttgaaa tggcggataa cggcacgctg
     1021 tttctggatg agatcggcga gttgtcgttg gcattgcagg ccaagctgct gagggtgttg
     1081 cagtatggcg atattcagcg cgttggcgat gaccgttgtt tgcgggtcga tgtgcgcgtg
     1141 ctggcggcga ctaaccgcga tttacgcgaa gaggtgctgg cagggcgatt ccgcgccgat
     1201 ttgtttcatc gcctgagcgt gtttccactt tcggtgccgc cgctgcgtga gcggggcgat
     1261 gatgtcattc tgctggcggg gtatttctgc gagcagtgtc gtttgcggca ggggctctcc
     1321 cgcgtggtat taagtgccgg agcgcgaaat ttactgcaac actacagttt tccgggaaac
     1381 gtgcgcgaac tggaacatgc tattcatcgg gcggtagttc tggcgagagc cacccgcagc
     1441 ggcgatgaag tgattcttga ggcgcaacat tttgcttttc ctgaggtgac gttgccgacg
     1501 ccagaagtgg cggcggtgcc cgttgttaag caaaacctgc gtgaagcgac agaagcgttc
     1561 cagcgtgaaa ctattcgtca ggcactggca caaaatcatc acaactgggc tgcctgcgcg
     1621 cggatgctgg aaaccgacgt cgccaacctg catcggctgg cgaaacgtct gggattgaag
     1681 gattaaatct catggtacgc gtgctagagg catcaaataa aacgaaaggc tcagtcgaaa
     1741 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat
     1801 ccgccgccct agacctaggg cctagggtac gggttttgct gcccgcaaac gggctgttct
     1861 ggtgttgcta gtttgttatc agaatcgcag atccggcttc agccggtttg ccggctgaaa
     1921 gcgctatttc ttccagaatt gccatgattt tttccccacg ggaggcgtca ctggctcccg
     1981 tgttgtcggc agctttgatt cgataagcag catcgcctgt ttcaggctgt ctatgtgtga
     2041 ctgttgagct gtaacaagtt gtctcaggtg ttcaatttca tgttctagtt gctttgtttt
     2101 actggtttca cctgttctat taggtgttac atgctgttca tctgttacat tgtcgatctg
     2161 ttcatggtga acagctttga atgcaccaaa aactcgtaaa agctctgatg tatctatctt
     2221 ttttacaccg ttttcatctg tgcatatgga cagttttccc tttgatatgt aacggtgaac
     2281 agttgttcta cttttgtttg ttagtcttga tgcttcactg atagatacaa gagccataag
     2341 aacctcagat ccttccgtat ttagccagta tgttctctag tgtggttcgt tgtttttgcg
     2401 tgagccatga gaacgaacca ttgagatcat acttactttg catgtcactc aaaaattttg
     2461 cctcaaaact ggtgagctga atttttgcag ttaaagcatc gtgtagtgtt tttcttagtc
     2521 cgttatgtag gtaggaatct gatgtaatgg ttgttggtat tttgtcacca ttcattttta
     2581 tctggttgtt ctcaagttcg gttacgagat ccatttgtct atctagttca acttggaaaa
     2641 tcaacgtatc agtcgggcgg cctcgcttat caaccaccaa tttcatattg ctgtaagtgt
     2701 ttaaatcttt acttattggt ttcaaaaccc attggttaag ccttttaaac tcatggtagt
     2761 tattttcaag cattaacatg aacttaaatt catcaaggct aatctctata tttgccttgt
     2821 gagttttctt ttgtgttagt tcttttaata accactcata aatcctcata gagtatttgt
     2881 tttcaaaaga cttaacatgt tccagattat attttatgaa tttttttaac tggaaaagat
     2941 aaggcaatat ctcttcacta aaaactaatt ctaatttttc gcttgagaac ttggcatagt
     3001 ttgtccactg gaaaatctca aagcctttaa ccaaaggatt cctgatttcc acagttctcg
     3061 tcatcagctc tctggttgct ttagctaata caccataagc attttcccta ctgatgttca
     3121 tcatctgagc gtattggtta taagtgaacg ataccgtccg ttctttcctt gtagggtttt
     3181 caatcgtggg gttgagtagt gccacacagc ataaaattag cttggtttca tgctccgtta
     3241 agtcatagcg actaatcgct agttcatttg ctttgaaaac aactaattca gacatacatc
     3301 tcaattggtc taggtgattt taatcactat accaattgag atgggctagt caatgataat
     3361 tactagtcct tttcctttga gttgtgggta tctgtaaatt ctgctagacc tttgctggaa
     3421 aacttgtaaa ttctgctaga ccctctgtaa attccgctag acctttgtgt gttttttttg
     3481 tttatattca agtggttata atttatagaa taaagaaaga ataaaaaaag ataaaaagaa
     3541 tagatcccag ccctgtgtat aactcactac tttagtcagt tccgcagtat tacaaaagga
     3601 tgtcgcaaac gctgtttgct cctctacaaa acagacctta aaaccctaaa ggcttaagta
     3661 gcaccctcgc aagctcgggc aaatcgctga atattccttt tgtctccgac catcaggcac
     3721 ctgagtcgct gtctttttcg tgacattcag ttcgctgcgc tcacggctct ggcagcgaat
     3781 gggggtaaat ggcactacag gcgcctttta tggattcatg caaggaaact acccataata
     3841 caagaaaagc ccgtcacggg cttctcaggg cgttttatgg cgggtctgct atgtggtgct
     3901 atctgacttt ttgctgttca gcagttcctg ccctctgatt ttccagtctg accacttcgg
     3961 attatcccgt gacaggtcag accttaaaac cctaaaggct taagtagcac cctcgcaagc
     4021 tcgggcaaat cgctgaatat tccttttgtc tccgaccatc aggcacctga gtcgctgtct
     4081 ttttcgtgac attcagttcg ctgcgctcac ggctctggca gtgaatgggg gtaaatggca
     4141 ctacaggcgc cttttatgga ttcatgcaag gaaactaccc ataatacaag aaaagcccgt
     4201 cacgggcttc tcagggcgtt ttatggcggg tctgctatgt ggtgctatct gactttttgc
     4261 tgttcagcag ttcctgccct ctgattttcc agtctgacca cttcggatta tcccgtgaca
     4321 ggtcattcag actggctaat gcacccagta aggcagcggt atcatcaaca ggcttacccg
     4381 tcttactgtc cctagtacta gtgcttggat tctcaccaat aaaaaacgcc cggcggcaac
     4441 cgagcgttct gaacaaatcc agatggagtt ctgaggtcat tactggatct atcaacagga
     4501 gtccaagcga gctcgtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     4561 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     4621 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     4681 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     4741 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     4801 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     4861 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     4921 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     4981 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     5041 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     5101 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     5161 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     5221 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     5281 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     5341 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     5401 cttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     5461 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     5521 ctgacgtctg tggcttttat gaaaatcaca cagtgatcac aaattttaaa cagagcacaa
     5581 aatgctgcct cgaaatgagg gcgggaaaat aaggttatca gccttgtttt ctccctcatt
     5641 acttgaagga tatgaagcta aaaccctttt ttataaagca tttgtccgaa ttcggacata
     5701 atcaaaaaag cttaattaag atcaatttga tctacatctc tttaaccaac aatatgtaag
     5761 atctcaacta cggaaaaact catctttgcc tcactgtcaa tttgactata gatattgtca
     5821 tatcgaccat ttgattgata gtcattttga ctactcatta atgggcataa ttttatttat
     5881 agagtaaaaa caatcagata aaaaactggc acgcaatctg caattagcaa gacatctttt
     5941 tagaacacgc taaagagggg aaaggtacca tgagagccct ggtagtcatc cgcctgtccc
     6001 gcgtcaccga tgctacgact tcaccggagc gtcagctgga gtcttgccag cagctctgcg
     6061 cccagcgcgg ctgggacgtc gtcggggtag cggaggatct ggacgtctcc ggggcggtcg
     6121 atccgttcga ccggaagcgc agaccgaacc tggcccggtg gctagcgttc gaggagcaac
     6181 cgttcgacgt gatcgtggcg taccgggtag accggttgac ccgatcgatc cggcatctgc
     6241 agcagctggt ccactgggcc gaggaccaca agaagctggt cgtctccgcg accgaagcgc
     6301 acttcgatac gacgacgccg tttgcggcgg tcgtcatcgc gcttatggga acggtggcgc
     6361 agatggaatt agaagcgatc aaagagcgga accgttcggc tgcgcatttc aatatccgcg
     6421 ccgggaaata ccgaggatcc ctgccgccgt ggggatacct gcctacgcgc gtggacgggg
     6481 agtggcggct ggtgccggac cctgtgcagc gagagcgcat cctcgaggtg tatcaccgcg
     6541 tcgtcgacaa ccacgagccg ctgcacctgg tggcccacga cctgaaccgg cgtggtgtcc
     6601 tgtcgccgaa ggactacttc gcgcagctgc aaggccgcga gccgcagggc cgggagtggt
     6661 cggctaccgc gctgaagcga tcgatgatct ccgaggcgat gctcgggtac gcgactctga
     6721 acggtaagac cgtccgagac gacgacggag ccccgctggt gcgggctgag ccgatcctga
     6781 cccgtgagca gctggaggcg ctgcgcgccg agctcgtgaa gacctcccgg gcgaagcccg
     6841 cggtgtctac cccgtcgctg ctgctgcggg tgttgttctg tgcggtgtgc ggggagcccg
     6901 cgtacaagtt cgccggggga ggacgtaagc acccgcgcta ccgctgccgc tcgatggggt
     6961 tcccgaagca ctgcgggaac ggcacggtgg cgatggccga gtgggacgcg ttctgcgagg
     7021 agcaggtgct ggatctgctc ggggacgcgg agcgtctgga gaaagtctgg gtagccggct
     7081 cggactccgc ggtcgaactc gcggaggtga acgcggagct ggtggacctg acgtcgctga
     7141 tcggctcccc ggcctaccgg gccggctctc cgcagcgaga agcactggat gcccgtattg
     7201 cggcgctggc cgcgcggcaa gaggagctgg agggtctaga ggctcgcccg tctggctggg
     7261 agtggcgcga gaccgggcag cggttcgggg actggtggcg ggagcaggac accgcggcaa
     7321 agaacacctg gcttcggtcg atgaacgttc ggctgacgtt cgacgtccgc ggcgggctga
     7381 ctcgcacgat cgacttcggg gatctgcagg agtacgagca gcatctcagg ctcggcagcg
     7441 tggtcgaacg gctacacacc gggatgtcga ggcctgcagc aaacgacgaa aactacgctg
     7501 cagcagttta gacacatggc atggatgaac tatacaaata acccggggga tcccatggta
     7561 cgcgtgctag aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
     7621 tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgc cctagaccta
     7681 g
//
