LOCUS       sequence-391290-       11282 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Synthetic gene circuit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11282)
  AUTHORS   Inda-Webb ME, Jimenez M, Liu Q, Phan NV, Ahn J, Steiger C, Wentworth
            A, Riaz A, Zirtiloglu T, Wong K, Ishida K, Fabian N, Jenkins J, 
            Kuosmanen J, Madani W, McNally R, Lai Y, Hayward A, Mimee M, Nadeau 
            P, Chandrakasan AP, Traverso G, Yazicigil RT, Lu TK
  TITLE     Sub-1.4 cm(3) capsule for detecting labile inflammatory biomarkers 
            in situ.
  JOURNAL   Nature. 2023 Aug;620(7973):386-392. doi: 10.1038/s41586-023-06369-x.
            Epub 2023 Jul 26.
  PUBMED    37495692
REFERENCE   2  (bases 1 to 11282)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-023-06369-x"; journalName: "Nature"; date: 
            "2023-08"; volume: "620"; issue: "7973"; pages: "386-392"
COMMENT     Sequence Label: TT lux sensor
FEATURES             Location/Qualifiers
     source          1..11282
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        39..73
                     /label=BBa_J23109
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.04"
     terminator      744..815
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      831..858
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     RBS             1066..1077
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             1084..2526
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC fatty acid reductase"
                     /label=LuxC
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTKKISFIINGQVEIFPEGDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MQHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
     CDS             2539..3462
                     /codon_start=1
                     /gene="luxD"
                     /product="LuxD acyltransferase"
                     /label=LuxD
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PNNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
                     FEHLTIATVNERRMRIEIENQAISLS"
     CDS             3511..4593
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             4608..5591
                     /codon_start=1
                     /gene="luxB"
                     /product="LuxB luciferase subunit"
                     /label=LuxB
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
                     FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILG
                     FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
                     TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
                     DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
                     AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT"
     CDS             5770..6882
                     /codon_start=1
                     /gene="luxE"
                     /product="LuxE"
                     /label=LuxE
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
                     RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
                     NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
                     VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
                     GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
                     MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
                     YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
     terminator      6983..7069
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(7614..8564)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      8612..8834
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(9678..9772)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(9802..10593,10594..10662))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10425..10444
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10663..10766)
                     /gene="bla"
                     /label=AmpR promoter
     RBS             11221..11232
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     primer_bind     join(11282,1..18)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ataggcgtat cacgaggccc tttcgtcttc acctcgagtt tacagctagc tcagtcctag
       61 ggactgtgct agcaccaaat atagagaatc acaaggatct attcaatgag cctggcgctg
      121 ccggtgtacc tgattgacga cgatgactcc gttcgccgtt ccctgcgttt catgctggaa
      181 agctacggcc tgaaaatcac tgacttcgat tctgctgaag cgttcttcac cgcggtagac
      241 ctgaccctgc cgggttgcgc actggtggac gtacgtatgc cgggcctgag cggcccacag
      301 ctgcacctgg aactggtgtc taagaacagc ccgctggccg tgatctatct gaccggtcac
      361 ggcgacgttc cgatggcggt tgaagcgctg aaactgggtg cggtagattt ctttcagaaa
      421 cctgcagacg gcgctaaact ggctgaagct gtggtcaaag ccctggaaca cgcgaaaacc
      481 cactaccaag acaaccagta cctggaaacc tatcaggctc tgaccccacg tgagcgcgaa
      541 atcctgaacc tgattgcgca aggtctgaaa aaccaggaaa tcgcggacaa cctgtgcatt
      601 gcgatgcgca ccgtagaaat ccaccgtgcg aacctgatga aaggtatgca ggtgggcagc
      661 ctggcagaga tgatgctgat ttactctcgt atcgcggagc gtctgcgcct ggaagataac
      721 cgttaataat ctagaccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
      781 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
      841 cgggtgggcc tttctgcgtt tatagtaaat cactgcataa ttcgtgtcgc tcaaggcgca
      901 ctcccgttct ggataatgtt ttttgcgccg acatcataac ggttctggca aatattctga
      961 aatgagctgg ttagctagtc aatatttgtt gcgctagatc aaatccacgc ctgatatgtg
     1021 gaaaaccact atagttatgc cgcctcgcct tttacagaat tcattaaaga ggagaaaggt
     1081 accatgacta aaaaaatttc attcattatt aacggccagg ttgaaatctt tcccgaaggt
     1141 gatgatttag tgcaatccat taattttggt gataatagtg tttacctgcc aatattgaat
     1201 gactctcatg taaaaaacat tattgattgt aatggaaata acgaattacg gttgcataac
     1261 attgtcaatt ttctctatac ggtagggcaa agatggaaaa atgaagaata ctcaagacgc
     1321 aggacataca ttcgtgactt aaaaaaatat atgggatatt cagaagaaat ggctaagcta
     1381 gaggccaatt ggatatctat gattttatgt tctaaaggcg gcctttatga tgttgtagaa
     1441 aatgaacttg gttctcgcca tatcatggat gaatggctac ctcaggatga aagttatgtt
     1501 cgggcttttc cgaaaggtaa atctgtacat ctgttggcag gtaatgttcc attatctggg
     1561 atcatgtcta tattacgcgc aattttaact aagaatcagt gtattataaa aacatcgtca
     1621 accgatcctt ttaccgctaa tgcattagcg ttaagtttta ttgatgtaga ccctaatcat
     1681 ccgataacgc gctctttatc tgttatatat tggccccacc aaggtgatac atcactcgca
     1741 aaagaaatta tgcaacatgc ggatgttatt gtcgcttggg gagggccaga tgcgattaat
     1801 tgggcggtag agcatgcgcc atcttatgct gatgtgatta aatttggttc taaaaagagt
     1861 ctttgcatta tcgataatcc tgttgatttg acgtccgcag cgacaggtgc ggctcatgat
     1921 gtttgttttt acgatcagcg agcttgtttt tctgcccaaa acatatatta catgggaaat
     1981 cattatgagg aatttaagtt agcgttgata gaaaaactta atctatatgc gcatatatta
     2041 ccgaatgcca aaaaagattt tgatgaaaag gcggcctatt ctttagttca aaaagaaagc
     2101 ttgtttgctg gattaaaagt agaggtggat attcatcaac gttggatgat tattgagtca
     2161 aatgcaggtg tggaatttaa tcaaccactt ggcagatgtg tgtaccttca tcacgtcgat
     2221 aatattgagc aaatattgcc ttatgttcaa aaaaataaga cgcaaaccat atctattttt
     2281 ccttgggagt catcatttaa atatcgagat gcgttagcat taaaaggtgc ggaaaggatt
     2341 gtagaagcag gaatgaataa catatttcga gttggtggat ctcatgacgg aatgcgaccg
     2401 ttgcaacgat tagtgacata tatttctcat gaaaggccat ctaactatac ggctaaggat
     2461 gttgcggttg aaatagaaca gactcgattc ctggaagaag ataagttcct tgtatttgtc
     2521 ccataatagg taaaaagtat ggaaaatgaa tcaaaatata aaaccatcga ccacgttatt
     2581 tgtgttgaag gaaataaaaa aattcatgtt tgggaaacgc tgccagaaga aaacagccca
     2641 aagagaaaga atgccattat tattgcgtct ggttttgccc gcaggatgga tcattttgct
     2701 ggtctggcgg aatatttatc gcggaatgga tttcatgtga tccgctatga ttcgcttcac
     2761 cacgttggat tgagttcagg gacaattgat gaatttacaa tgtctatagg aaagcagagc
     2821 ttgttagcag tggttgattg gttaactaca cgaaaaataa ataacttcgg tatgttggct
     2881 tcaagcttat ctgcgcggat agcttatgca agcctatctg aaatcaatgc ttcgttttta
     2941 atcaccgcag tcggtgttgt taacttaaga tattctcttg aaagagcttt agggtttgat
     3001 tatctcagtc tacccattaa tgaattgccg aataatctag attttgaagg ccataaattg
     3061 ggtgctgaag tctttgcgag agattgtctt gattttggtt gggaagattt agcttctaca
     3121 attaataaca tgatgtatct tgatataccg tttattgctt ttactgcaaa taacgataat
     3181 tgggtcaagc aagatgaagt tatcacattg ttatcaaata ttcgtagtaa tcgatgcaag
     3241 atatattctt tgttaggaag ttcgcatgac ttgagtgaaa atttagtggt cctgcgcaat
     3301 ttttatcaat cggttacgaa agccgctatc gcgatggata atgatcatct ggatattgat
     3361 gttgatatta ctgaaccgtc atttgaacat ttaactattg cgacagtcaa tgaacgccga
     3421 atgagaattg agattgaaaa tcaagcaatt tctctgtctt aaaatctatt gagatattct
     3481 atcactcaaa tagcaatata aggactctct atgaaatttg gaaacttttt gcttacatac
     3541 caacctcccc aattttctca aacagaggta atgaaacgtt tggttaaatt aggtcgcatc
     3601 tctgaggagt gtggttttga taccgtatgg ttactggagc atcatttcac ggagtttggt
     3661 ttgcttggta acccttatgt cgctgctgca tatttacttg gcgcgactaa aaaattgaat
     3721 gtaggaactg ccgctattgt tcttcccaca gcccatccag tacgccaact tgaagatgtg
     3781 aatttattgg atcaaatgtc aaaaggacga tttcggtttg gtatttgccg agggctttac
     3841 aacaaggact ttcgcgtatt cggcacagat atgaataaca gtcgcgcctt agcggaatgc
     3901 tggtacgggc tgataaagaa tggcatgaca gagggatata tggaagctga taatgaacat
     3961 atcaagttcc ataaggtaaa agtaaacccc gcggcgtata gcagaggtgg cgcaccggtt
     4021 tatgtggtgg ctgaatcagc ttcgacgact gagtgggctg ctcaatttgg cctaccgatg
     4081 atattaagtt ggattataaa tactaacgaa aagaaagcac aacttgagct ttataatgaa
     4141 gtggctcaag aatatgggca cgatattcat aatatcgacc attgcttatc atatataaca
     4201 tctgtagatc atgactcaat taaagcgaaa gagatttgcc ggaaatttct ggggcattgg
     4261 tatgattctt atgtgaatgc tacgactatt tttgatgatt cagaccaaac aagaggttat
     4321 gatttcaata aagggcagtg gcgtgacttt gtattaaaag gacataaaga tactaatcgc
     4381 cgtattgatt acagttacga aatcaatccc gtgggaacgc cgcaggaatg tattgacata
     4441 attcaaaaag acattgatgc tacaggaata tcaaatattt gttgtggatt tgaagctaat
     4501 ggaacagtag acgaaattat tgcttccatg aagctcttcc agtctgatgt catgccattt
     4561 cttaaagaaa aacaacgttc gctattatat tagctaagga gaaagaaatg aaatttggat
     4621 tgttcttcct taacttcatc aattcaacaa ctgttcaaga acaaagtata gttcgcatgc
     4681 aggaaataac ggagtatgtt gataagttga attttgaaca gattttagtg tatgaaaatc
     4741 atttttcaga taatggtgtt gtcggcgctc ctctgactgt ttctggtttt ctgctcggtt
     4801 taacagagaa aattaaaatt ggttcattaa atcacatcat tacaactcat catcctgtcc
     4861 gcatagcgga ggaagcttgc ttattggatc agttaagtga agggagattt attttagggt
     4921 ttagtgattg cgaaaaaaaa gatgaaatgc atttttttaa tcgcccggtt gaatatcaac
     4981 agcaactatt tgaagagtgt tatgaaatca ttaacgatgc tttaacaaca ggctattgta
     5041 atccagataa cgatttttat agcttcccta aaatatctgt aaatccccat gcttatacgc
     5101 caggcggacc tcggaaatat gtaacagcaa ccagtcatca tattgttgag tgggcggcca
     5161 aaaaaggtat tcctctcatc tttaagtggg atgattctaa tgatgttaga tatgaatatg
     5221 ctgaaagata taaagccgtt gcggataaat atgacgttga cctatcagag atagaccatc
     5281 agttaatgat attagttaac tataacgaag atagtaataa agctaaacaa gagacgcgtg
     5341 catttattag tgattatgtt cttgaaatgc accctaatga aaatttcgaa aataaacttg
     5401 aagaaataat tgcagaaaac gctgtcggaa attatacgga gtgtataact gcggctaagt
     5461 tggcaattga aaagtgtggt gcgaaaagtg tattgctgtc ctttgaacca atgaatgatt
     5521 tgatgagcca aaaaaatgta atcaatattg ttgatgataa tattaagaag taccacatgg
     5581 aatataccta atagatttcg agttgcagcg aggcggcaag tgaacgaatc cccaggagca
     5641 tagataacta tgtgactggg gtgagtgaaa gcagccaaca aagcagcagc ttgaaagatg
     5701 aagggtataa aagagtatga cagcagtgct gccatacttt ctaatattat cttgaggagt
     5761 aaaacaggta tgacttcata tgttgataaa caagaaatta cagcaagctc agaaattgat
     5821 gatttgattt tttcgagcga tccattagtg tggtcttacg acgagcagga aaaaatcaga
     5881 aagaaacttg tgcttgatgc atttcgtaat cattataaac attgtcgaga atatcgtcac
     5941 tactgtcagg cacacaaagt agatgacaat attacggaaa ttgatgacat acctgtattc
     6001 ccaacatcgg tttttaagtt tactcgctta ttaacttctc aggaaaacga gattgaaagt
     6061 tggtttacca gtagcggcac gaatggttta aaaagtcagg tggcgcgtga cagattaagt
     6121 attgagagac tcttaggctc tgtgagttat ggcatgaaat atgttggtag ttggtttgat
     6181 catcaaatag aattagtcaa tttgggacca gatagattta atgctcataa tatttggttt
     6241 aaatatgtta tgagtttggt ggaattgtta tatcctacga catttaccgt aacagaagaa
     6301 cgaatagatt ttgttaaaac attgaatagt cttgaacgaa taaaaaatca agggaaagat
     6361 ctttgtctta ttggttcgcc atactttatt tatttactct gccattatat gaaagataaa
     6421 aaaatctcat tttctggaga taaaagcctt tatatcataa ccggaggcgg ctggaaaagt
     6481 tacgaaaaag aatctctgaa acgtgatgat ttcaatcatc ttttatttga tactttcaat
     6541 ctcagtgata ttagtcagat ccgagatata tttaatcaag ttgaactcaa cacttgtttc
     6601 tttgaggatg aaatgcagcg taaacatgtt ccgccgtggg tatatgcgcg agcgcttgat
     6661 cctgaaacgt tgaaacctgt acctgatgga acgccggggt tgatgagtta tatggatgcg
     6721 tcagcaacca gttatccagc atttattgtt accgatgatg tcgggataat tagcagagaa
     6781 tatggtaagt atcccggcgt gctcgttgaa attttacgtc gcgtcaatac gaggacgcag
     6841 aaagggtgtg ctttaagctt aaccgaagcg tttgatagtt gaaagcttga tatcgaattc
     6901 ctgcccaatt attgaagggg agcgggaaac cgctcccctt tttttgtttc tggtctcccc
     6961 atggtacgcg tgctagaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
     7021 tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgcccta
     7081 gacctagggc ctagggtacg ggttttgctg cccgcaaacg ggctgttctg gtgttgctag
     7141 tttgttatca gaatcgcaga tccggcttca gccggtttgc cggctgaaag cgctatttct
     7201 tccagaattg ccatgatttt ttccccacgg gaggcgtcac tggctcccgt gttgtcggca
     7261 gctttgattc gataagcagc atcgcctgtt tcaggctgtc tatgtgtgac tgttgagctg
     7321 taacaagttg tctcaggtgt tcaatttcat gttctagttg ctttgtttta ctggtttcac
     7381 ctgttctatt aggtgttaca tgctgttcat ctgttacatt gtcgatctgt tcatggtgaa
     7441 cagctttgaa tgcaccaaaa actcgtaaaa gctctgatgt atctatcttt tttacaccgt
     7501 tttcatctgt gcatatggac agttttccct ttgatatgta acggtgaaca gttgttctac
     7561 ttttgtttgt tagtcttgat gcttcactga tagatacaag agccataaga acctcagatc
     7621 cttccgtatt tagccagtat gttctctagt gtggttcgtt gtttttgcgt gagccatgag
     7681 aacgaaccat tgagatcata cttactttgc atgtcactca aaaattttgc ctcaaaactg
     7741 gtgagctgaa tttttgcagt taaagcatcg tgtagtgttt ttcttagtcc gttatgtagg
     7801 taggaatctg atgtaatggt tgttggtatt ttgtcaccat tcatttttat ctggttgttc
     7861 tcaagttcgg ttacgagatc catttgtcta tctagttcaa cttggaaaat caacgtatca
     7921 gtcgggcggc ctcgcttatc aaccaccaat ttcatattgc tgtaagtgtt taaatcttta
     7981 cttattggtt tcaaaaccca ttggttaagc cttttaaact catggtagtt attttcaagc
     8041 attaacatga acttaaattc atcaaggcta atctctatat ttgccttgtg agttttcttt
     8101 tgtgttagtt cttttaataa ccactcataa atcctcatag agtatttgtt ttcaaaagac
     8161 ttaacatgtt ccagattata ttttatgaat ttttttaact ggaaaagata aggcaatatc
     8221 tcttcactaa aaactaattc taatttttcg cttgagaact tggcatagtt tgtccactgg
     8281 aaaatctcaa agcctttaac caaaggattc ctgatttcca cagttctcgt catcagctct
     8341 ctggttgctt tagctaatac accataagca ttttccctac tgatgttcat catctgagcg
     8401 tattggttat aagtgaacga taccgtccgt tctttccttg tagggttttc aatcgtgggg
     8461 ttgagtagtg ccacacagca taaaattagc ttggtttcat gctccgttaa gtcatagcga
     8521 ctaatcgcta gttcatttgc tttgaaaaca actaattcag acatacatct caattggtct
     8581 aggtgatttt aatcactata ccaattgaga tgggctagtc aatgataatt actagtcctt
     8641 ttcctttgag ttgtgggtat ctgtaaattc tgctagacct ttgctggaaa acttgtaaat
     8701 tctgctagac cctctgtaaa ttccgctaga cctttgtgtg ttttttttgt ttatattcaa
     8761 gtggttataa tttatagaat aaagaaagaa taaaaaaaga taaaaagaat agatcccagc
     8821 cctgtgtata actcactact ttagtcagtt ccgcagtatt acaaaaggat gtcgcaaacg
     8881 ctgtttgctc ctctacaaaa cagaccttaa aaccctaaag gcttaagtag caccctcgca
     8941 agctcgggca aatcgctgaa tattcctttt gtctccgacc atcaggcacc tgagtcgctg
     9001 tctttttcgt gacattcagt tcgctgcgct cacggctctg gcagtgaatg ggggtaaatg
     9061 gcactacagg cgccttttat ggattcatgc aaggaaacta cccataatac aagaaaagcc
     9121 cgtcacgggc ttctcagggc gttttatggc gggtctgcta tgtggtgcta tctgactttt
     9181 tgctgttcag cagttcctgc cctctgattt tccagtctga ccacttcgga ttatcccgtg
     9241 acaggtcaga ccttaaaacc ctaaaggctt aagtagcacc ctcgcaagct cgggcaaatc
     9301 gctgaatatt ccttttgtct ccgaccatca ggcacctgag tcgctgtctt tttcgtgaca
     9361 ttcagttcgc tgcgctcacg gctctggcag tgaatggggg taaatggcac tacaggcgcc
     9421 ttttatggat tcatgcaagg aaactaccca taatacaaga aaagcccgtc acgggcttct
     9481 cagggcgttt tatggcgggt ctgctatgtg gtgctatctg actttttgct gttcagcagt
     9541 tcctgccctc tgattttcca gtctgaccac ttcggattat cccgtgacag gtcattcaga
     9601 ctggctaatg cacccagtaa ggcagcggta tcatcaacag gcttacccgt cttactgtcc
     9661 ctagtactag tgcttggatt ctcaccaata aaaaacgccc ggcggcaacc gagcgttctg
     9721 aacaaatcca gatggagttc tgaggtcatt actggatcta tcaacaggag tccaagcgag
     9781 ctcgtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     9841 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     9901 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     9961 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
    10021 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
    10081 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
    10141 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
    10201 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
    10261 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
    10321 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
    10381 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
    10441 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
    10501 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    10561 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    10621 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc ttttcaatat
    10681 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
    10741 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctgt
    10801 ggcttttatg aaaatcacac agtgatcaca aattttaaac agagcacaaa atgctgcctc
    10861 gaaatgaggg cgggaaaata aggttatcag ccttgttttc tccctcatta cttgaaggat
    10921 atgaagctaa aacccttttt tataaagcat ttgtccgaat tcggacataa tcaaaaaagc
    10981 ttaattaaga tcaatttgat ctacatctct ttaaccaaca atatgtaaga tctcaactac
    11041 ggaaaaactc atctttgcct cactgtcaat ttgactatag atattgtcat atcgaccatt
    11101 tgattgatag tcattttgac tactcattaa tgggcataat tttatttata gagtaaaaac
    11161 aatcagataa aaaactggca cgcaatctgc aattagcaag acatcttttt agaacacgct
    11221 aaagaggaga aaggtaccat gacggaaaaa ctcatctttg cctcactgtc aatttgacta
    11281 ta
//
