LOCUS       sequence-391293-        9808 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Synthetic gene circuit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9808)
  AUTHORS   Inda-Webb ME, Jimenez M, Liu Q, Phan NV, Ahn J, Steiger C, Wentworth
            A, Riaz A, Zirtiloglu T, Wong K, Ishida K, Fabian N, Jenkins J, 
            Kuosmanen J, Madani W, McNally R, Lai Y, Hayward A, Mimee M, Nadeau 
            P, Chandrakasan AP, Traverso G, Yazicigil RT, Lu TK
  TITLE     Sub-1.4 cm(3) capsule for detecting labile inflammatory biomarkers 
            in situ.
  JOURNAL   Nature. 2023 Aug;620(7973):386-392. doi: 10.1038/s41586-023-06369-x.
            Epub 2023 Jul 26.
  PUBMED    37495692
REFERENCE   2  (bases 1 to 9808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-023-06369-x"; journalName: "Nature"; date: 
            "2023-08"; volume: "620"; issue: "7973"; pages: "386-392"
COMMENT     Sequence Label: NO lux Sensor
FEATURES             Location/Qualifiers
     source          1..9808
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(508..1458)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      1506..1728
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(2226..2320)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(2350..3141,3142..3210))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2973..2992
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3211..3314)
                     /gene="bla"
                     /label=AmpR promoter
     RBS             3769..3780
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             3787..5229
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC fatty acid reductase"
                     /label=LuxC
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTKKISFIINGQVEIFPEGDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MQHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
     CDS             5242..6165
                     /codon_start=1
                     /gene="luxD"
                     /product="LuxD acyltransferase"
                     /label=LuxD
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PNNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
                     FEHLTIATVNERRMRIEIENQAISLS"
     CDS             6214..7296
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             7311..8294
                     /codon_start=1
                     /gene="luxB"
                     /product="LuxB luciferase subunit"
                     /label=LuxB
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
                     FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILG
                     FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
                     TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
                     DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
                     AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT"
     CDS             8473..9585
                     /codon_start=1
                     /gene="luxE"
                     /product="LuxE"
                     /label=LuxE
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
                     RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
                     NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
                     VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
                     GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
                     MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
                     YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
     terminator      9685..9771
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gctgcccgca aacgggctgt tctggtgttg ctagtttgtt atcagaatcg cagatccggc
       61 ttcagccggt ttgccggctg aaagcgctat ttcttccaga attgccatga ttttttcccc
      121 acgggaggcg tcactggctc ccgtgttgtc ggcagctttg attcgataag cagcatcgcc
      181 tgtttcaggc tgtctatgtg tgactgttga gctgtaacaa gttgtctcag gtgttcaatt
      241 tcatgttcta gttgctttgt tttactggtt tcacctgttc tattaggtgt tacatgctgt
      301 tcatctgtta cattgtcgat ctgttcatgg tgaacagctt tgaatgcacc aaaaactcgt
      361 aaaagctctg atgtatctat cttttttaca ccgttttcat ctgtgcatat ggacagtttt
      421 ccctttgata tgtaacggtg aacagttgtt ctacttttgt ttgttagtct tgatgcttca
      481 ctgatagata caagagccat aagaacctca gatccttccg tatttagcca gtatgttctc
      541 tagtgtggtt cgttgttttt gcgtgagcca tgagaacgaa ccattgagat catacttact
      601 ttgcatgtca ctcaaaaatt ttgcctcaaa actggtgagc tgaatttttg cagttaaagc
      661 atcgtgtagt gtttttctta gtccgttatg taggtaggaa tctgatgtaa tggttgttgg
      721 tattttgtca ccattcattt ttatctggtt gttctcaagt tcggttacga gatccatttg
      781 tctatctagt tcaacttgga aaatcaacgt atcagtcggg cggcctcgct tatcaaccac
      841 caatttcata ttgctgtaag tgtttaaatc tttacttatt ggtttcaaaa cccattggtt
      901 aagcctttta aactcatggt agttattttc aagcattaac atgaacttaa attcatcaag
      961 gctaatctct atatttgcct tgtgagtttt cttttgtgtt agttctttta ataaccactc
     1021 ataaatcctc atagagtatt tgttttcaaa agacttaaca tgttccagat tatattttat
     1081 gaattttttt aactggaaaa gataaggcaa tatctcttca ctaaaaacta attctaattt
     1141 ttcgcttgag aacttggcat agtttgtcca ctggaaaatc tcaaagcctt taaccaaagg
     1201 attcctgatt tccacagttc tcgtcatcag ctctctggtt gctttagcta atacaccata
     1261 agcattttcc ctactgatgt tcatcatctg agcgtattgg ttataagtga acgataccgt
     1321 ccgttctttc cttgtagggt tttcaatcgt ggggttgagt agtgccacac agcataaaat
     1381 tagcttggtt tcatgctccg ttaagtcata gcgactaatc gctagttcat ttgctttgaa
     1441 aacaactaat tcagacatac atctcaattg gtctaggtga ttttaatcac tataccaatt
     1501 gagatgggct agtcaatgat aattactagt ccttttcctt tgagttgtgg gtatctgtaa
     1561 attctgctag acctttgctg gaaaacttgt aaattctgct agaccctctg taaattccgc
     1621 tagacctttg tgtgtttttt ttgtttatat tcaagtggtt ataatttata gaataaagaa
     1681 agaataaaaa aagataaaaa gaatagatcc cagccctgtg tataactcac tactttagtc
     1741 agttccgcag tattacaaaa ggatgtcgca aacgctgttt gctcctctac aaaacagacc
     1801 ttaaaaccct aaaggcttaa gtagcaccct cgcaagctcg ggcaaatcgc tgaatattcc
     1861 ttttgtctcc gaccatcagg cacctgagtc gctgtctttt tcgtgacatt cagttcgctg
     1921 cgctcacggc tctggcagtg aatgggggta aatggcacta caggcgcctt ttatggattc
     1981 atgcaaggaa actacccata atacaagaaa agcccgtcac gggcttctca gggcgtttta
     2041 tggcgggtct gctatgtggt gctatctgac tttttgctgt tcagcagttc ctgccctctg
     2101 attttccagt ctgaccactt cggattatcc cgtgacaggt cattcagact ggctaatgca
     2161 cccagtaagg cagcggtatc atcaacaggc ttacccgtct tactgtccct agtactagtg
     2221 cttggattct caccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga
     2281 tggagttctg aggtcattac tggatctatc aacaggagtc caagcgagct cgtaaacttg
     2341 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     2401 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     2461 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     2521 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     2581 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     2641 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     2701 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     2761 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     2821 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     2881 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     2941 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     3001 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     3061 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     3121 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     3181 aagggcgaca cggaaatgtt gaatactcat actcttcctt ttcaatatta ttgaagcatt
     3241 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     3301 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctgtgg cttttatgaa
     3361 aatcacacag tgatcacaaa ttttaaacag agcacaaaat gctgcctcga aatgagggcg
     3421 ggaaaataag gttatcagcc ttgttttctc cctcattact tgaaggatat gaagctaaaa
     3481 ccctttttta taaagcattt gtccgaattc ggacataatc aaaaaagctt aattaagatc
     3541 aatttgatct acatctcttt aaccaacaat atgtaagatc tcaactacgg aaaaactcat
     3601 ctttgcctca ctgtcaattt gactatagat attgtcatat cgaccatttg attgatagtc
     3661 attttgacta ctcattaatg ggcataattt tatttataga gtaaaaacaa tcagataaaa
     3721 aactggcacg caatctgcaa ttagcaagac atctttttag aacacattaa agaggagaaa
     3781 ggtaccatga ctaaaaaaat ttcattcatt attaacggcc aggttgaaat ctttcccgaa
     3841 ggtgatgatt tagtgcaatc cattaatttt ggtgataata gtgtttacct gccaatattg
     3901 aatgactctc atgtaaaaaa cattattgat tgtaatggaa ataacgaatt acggttgcat
     3961 aacattgtca attttctcta tacggtaggg caaagatgga aaaatgaaga atactcaaga
     4021 cgcaggacat acattcgtga cttaaaaaaa tatatgggat attcagaaga aatggctaag
     4081 ctagaggcca attggatatc tatgatttta tgttctaaag gcggccttta tgatgttgta
     4141 gaaaatgaac ttggttctcg ccatatcatg gatgaatggc tacctcagga tgaaagttat
     4201 gttcgggctt ttccgaaagg taaatctgta catctgttgg caggtaatgt tccattatct
     4261 gggatcatgt ctatattacg cgcaatttta actaagaatc agtgtattat aaaaacatcg
     4321 tcaaccgatc cttttaccgc taatgcatta gcgttaagtt ttattgatgt agaccctaat
     4381 catccgataa cgcgctcttt atctgttata tattggcccc accaaggtga tacatcactc
     4441 gcaaaagaaa ttatgcaaca tgcggatgtt attgtcgctt ggggagggcc agatgcgatt
     4501 aattgggcgg tagagcatgc gccatcttat gctgatgtga ttaaatttgg ttctaaaaag
     4561 agtctttgca ttatcgataa tcctgttgat ttgacgtccg cagcgacagg tgcggctcat
     4621 gatgtttgtt tttacgatca gcgagcttgt ttttctgccc aaaacatata ttacatggga
     4681 aatcattatg aggaatttaa gttagcgttg atagaaaaac ttaatctata tgcgcatata
     4741 ttaccgaatg ccaaaaaaga ttttgatgaa aaggcggcct attctttagt tcaaaaagaa
     4801 agcttgtttg ctggattaaa agtagaggtg gatattcatc aacgttggat gattattgag
     4861 tcaaatgcag gtgtggaatt taatcaacca cttggcagat gtgtgtacct tcatcacgtc
     4921 gataatattg agcaaatatt gccttatgtt caaaaaaata agacgcaaac catatctatt
     4981 tttccttggg agtcatcatt taaatatcga gatgcgttag cattaaaagg tgcggaaagg
     5041 attgtagaag caggaatgaa taacatattt cgagttggtg gatctcatga cggaatgcga
     5101 ccgttgcaac gattagtgac atatatttct catgaaaggc catctaacta tacggctaag
     5161 gatgttgcgg ttgaaataga acagactcga ttcctggaag aagataagtt ccttgtattt
     5221 gtcccataat aggtaaaaag tatggaaaat gaatcaaaat ataaaaccat cgaccacgtt
     5281 atttgtgttg aaggaaataa aaaaattcat gtttgggaaa cgctgccaga agaaaacagc
     5341 ccaaagagaa agaatgccat tattattgcg tctggttttg cccgcaggat ggatcatttt
     5401 gctggtctgg cggaatattt atcgcggaat ggatttcatg tgatccgcta tgattcgctt
     5461 caccacgttg gattgagttc agggacaatt gatgaattta caatgtctat aggaaagcag
     5521 agcttgttag cagtggttga ttggttaact acacgaaaaa taaataactt cggtatgttg
     5581 gcttcaagct tatctgcgcg gatagcttat gcaagcctat ctgaaatcaa tgcttcgttt
     5641 ttaatcaccg cagtcggtgt tgttaactta agatattctc ttgaaagagc tttagggttt
     5701 gattatctca gtctacccat taatgaattg ccgaataatc tagattttga aggccataaa
     5761 ttgggtgctg aagtctttgc gagagattgt cttgattttg gttgggaaga tttagcttct
     5821 acaattaata acatgatgta tcttgatata ccgtttattg cttttactgc aaataacgat
     5881 aattgggtca agcaagatga agttatcaca ttgttatcaa atattcgtag taatcgatgc
     5941 aagatatatt ctttgttagg aagttcgcat gacttgagtg aaaatttagt ggtcctgcgc
     6001 aatttttatc aatcggttac gaaagccgct atcgcgatgg ataatgatca tctggatatt
     6061 gatgttgata ttactgaacc gtcatttgaa catttaacta ttgcgacagt caatgaacgc
     6121 cgaatgagaa ttgagattga aaatcaagca atttctctgt cttaaaatct attgagatat
     6181 tctatcactc aaatagcaat ataaggactc tctatgaaat ttggaaactt tttgcttaca
     6241 taccaacctc cccaattttc tcaaacagag gtaatgaaac gtttggttaa attaggtcgc
     6301 atctctgagg agtgtggttt tgataccgta tggttactgg agcatcattt cacggagttt
     6361 ggtttgcttg gtaaccctta tgtcgctgct gcatatttac ttggcgcgac taaaaaattg
     6421 aatgtaggaa ctgccgctat tgttcttccc acagcccatc cagtacgcca acttgaagat
     6481 gtgaatttat tggatcaaat gtcaaaagga cgatttcggt ttggtatttg ccgagggctt
     6541 tacaacaagg actttcgcgt attcggcaca gatatgaata acagtcgcgc cttagcggaa
     6601 tgctggtacg ggctgataaa gaatggcatg acagagggat atatggaagc tgataatgaa
     6661 catatcaagt tccataaggt aaaagtaaac cccgcggcgt atagcagagg tggcgcaccg
     6721 gtttatgtgg tggctgaatc agcttcgacg actgagtggg ctgctcaatt tggcctaccg
     6781 atgatattaa gttggattat aaatactaac gaaaagaaag cacaacttga gctttataat
     6841 gaagtggctc aagaatatgg gcacgatatt cataatatcg accattgctt atcatatata
     6901 acatctgtag atcatgactc aattaaagcg aaagagattt gccggaaatt tctggggcat
     6961 tggtatgatt cttatgtgaa tgctacgact atttttgatg attcagacca aacaagaggt
     7021 tatgatttca ataaagggca gtggcgtgac tttgtattaa aaggacataa agatactaat
     7081 cgccgtattg attacagtta cgaaatcaat cccgtgggaa cgccgcagga atgtattgac
     7141 ataattcaaa aagacattga tgctacagga atatcaaata tttgttgtgg atttgaagct
     7201 aatggaacag tagacgaaat tattgcttcc atgaagctct tccagtctga tgtcatgcca
     7261 tttcttaaag aaaaacaacg ttcgctatta tattagctaa ggagaaagaa atgaaatttg
     7321 gattgttctt ccttaacttc atcaattcaa caactgttca agaacaaagt atagttcgca
     7381 tgcaggaaat aacggagtat gttgataagt tgaattttga acagatttta gtgtatgaaa
     7441 atcatttttc agataatggt gttgtcggcg ctcctctgac tgtttctggt tttctgctcg
     7501 gtttaacaga gaaaattaaa attggttcat taaatcacat cattacaact catcatcctg
     7561 tccgcatagc ggaggaagct tgcttattgg atcagttaag tgaagggaga tttattttag
     7621 ggtttagtga ttgcgaaaaa aaagatgaaa tgcatttttt taatcgcccg gttgaatatc
     7681 aacagcaact atttgaagag tgttatgaaa tcattaacga tgctttaaca acaggctatt
     7741 gtaatccaga taacgatttt tatagcttcc ctaaaatatc tgtaaatccc catgcttata
     7801 cgccaggcgg acctcggaaa tatgtaacag caaccagtca tcatattgtt gagtgggcgg
     7861 ccaaaaaagg tattcctctc atctttaagt gggatgattc taatgatgtt agatatgaat
     7921 atgctgaaag atataaagcc gttgcggata aatatgacgt tgacctatca gagatagacc
     7981 atcagttaat gatattagtt aactataacg aagatagtaa taaagctaaa caagagacgc
     8041 gtgcatttat tagtgattat gttcttgaaa tgcaccctaa tgaaaatttc gaaaataaac
     8101 ttgaagaaat aattgcagaa aacgctgtcg gaaattatac ggagtgtata actgcggcta
     8161 agttggcaat tgaaaagtgt ggtgcgaaaa gtgtattgct gtcctttgaa ccaatgaatg
     8221 atttgatgag ccaaaaaaat gtaatcaata ttgttgatga taatattaag aagtaccaca
     8281 tggaatatac ctaatagatt tcgagttgca gcgaggcggc aagtgaacga atccccagga
     8341 gcatagataa ctatgtgact ggggtgagtg aaagcagcca acaaagcagc agcttgaaag
     8401 atgaagggta taaaagagta tgacagcagt gctgccatac tttctaatat tatcttgagg
     8461 agtaaaacag gtatgacttc atatgttgat aaacaagaaa ttacagcaag ctcagaaatt
     8521 gatgatttga ttttttcgag cgatccatta gtgtggtctt acgacgagca ggaaaaaatc
     8581 agaaagaaac ttgtgcttga tgcatttcgt aatcattata aacattgtcg agaatatcgt
     8641 cactactgtc aggcacacaa agtagatgac aatattacgg aaattgatga catacctgta
     8701 ttcccaacat cggtttttaa gtttactcgc ttattaactt ctcaggaaaa cgagattgaa
     8761 agttggttta ccagtagcgg cacgaatggt ttaaaaagtc aggtggcgcg tgacagatta
     8821 agtattgaga gactcttagg ctctgtgagt tatggcatga aatatgttgg tagttggttt
     8881 gatcatcaaa tagaattagt caatttggga ccagatagat ttaatgctca taatatttgg
     8941 tttaaatatg ttatgagttt ggtggaattg ttatatccta cgacatttac cgtaacagaa
     9001 gaacgaatag attttgttaa aacattgaat agtcttgaac gaataaaaaa tcaagggaaa
     9061 gatctttgtc ttattggttc gccatacttt atttatttac tctgccatta tatgaaagat
     9121 aaaaaaatct cattttctgg agataaaagc ctttatatca taaccggagg cggctggaaa
     9181 agttacgaaa aagaatctct gaaacgtgat gatttcaatc atcttttatt tgatactttc
     9241 aatctcagtg atattagtca gatccgagat atatttaatc aagttgaact caacacttgt
     9301 ttctttgagg atgaaatgca gcgtaaacat gttccgccgt gggtatatgc gcgagcgctt
     9361 gatcctgaaa cgttgaaacc tgtacctgat ggaacgccgg ggttgatgag ttatatggat
     9421 gcgtcagcaa ccagttatcc agcatttatt gttaccgatg atgtcgggat aattagcaga
     9481 gaatatggta agtatcccgg cgtgctcgtt gaaattttac gtcgcgtcaa tacgaggacg
     9541 cagaaagggt gtgctttaag cttaaccgaa gcgtttgata gttgaaagct tgatatcgaa
     9601 ttcctgccca attattgaag gggagcggaa accgctcccc tttttttgtt tctggtctcc
     9661 ccatggtacg cgtgctagag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
     9721 tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccgccc
     9781 tagacctagg gcctagggta cgggtttt
//
