LOCUS       sequence-391302-       10512 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pDEL63; transgenic construct to express GFP-CAAX under nrg1 type II 
            regulatory region control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10512)
  AUTHORS   Lysko DE, Talbot WS
  TITLE     Unmyelinated sensory neurons use Neuregulin signals to promote 
            myelination of interneurons in the CNS.
  JOURNAL   Cell Rep. 2022 Nov 15;41(7):111669. doi: 
            10.1016/j.celrep.2022.111669.
  PUBMED    36384112
REFERENCE   2  (bases 1 to 10512)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2022-11-15"; volume: "41(7):111669. doi"; pages: " 
            10.1016/j.celrep.2022.111669"
COMMENT     Sequence Label: pTol2_(-3kb)nrg1_typeII:GFP-CAAX
FEATURES             Location/Qualifiers
     source          1..10512
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(618..629,630..792)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     1165..1181
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1201..1221
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    4333..4357
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(4360..4362,4363..4365,4366..5076)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4360..4362,4363..4365,4366..5076)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     protein_bind    complement(5156..5180)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5604..5624)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(5632..5648)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    5664..5798
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(6100..6813,6814..6816,6817..6819))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(6103..6813,6814..6816,6817..6819))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    join(8042..8229,8230..8241)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(8275..8291)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8275..8291)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(8328..8348)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8328..8346)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8367..8383)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8380..8402)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8391..8407
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8415..8421,8422..8439,8440..8445))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8460..8481
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8598..8615)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8769..9357)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8849..8868)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9528..10319,10320..10388))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10151..10170
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10389..10493)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      301 ggagcccccg atttagagct tgacggggaa aggctggcta agaactcatc agcctccccg
      361 gtccatctac ccacgtacca atgcaccaat tggccacaat gacggctact acatggtgcc
      421 attccttcct ctttatagga atggagacta cctcctgtcc aacaatgctc ttggatacga
      481 gtacgcctac ctgttggacc caggtcattg cacaacacca gaaatgccct ctgatctgca
      541 aaagacgtga atatctgttc agacacccat atccactctg ttccacacag gtcagaggtt
      601 tgtccaggag ttcttgacag aggtgtaaaa agtactcaaa aattttactc aagtgaaagt
      661 acaagtactt agggaaaatt ttactcaatt aaaagtaaaa gtatctggct agaatcttac
      721 ttgagtaaaa gtaaaaaagt actccattaa aattgtactt gagtattaag gaagtaaaag
      781 taaaagcaag aaagaaaact agagattctt gtttaagctt ttaatctcaa aaaacattaa
      841 atgaaatgca tacaaggttt tatcctgctt tagaactgtt tgtatttaat tatcaaacta
      901 taagacagac aatctaatgc cagtacacgc tactcaaagt tgtaaaacct cagatttaac
      961 ttcagtagaa gctgattctc aaaattgtta gtgtcaagcc tagctctttt ggggctgaaa
     1021 agcaatcctg cagtgctgaa aagcctctca caggcagccg atgcgggaag aggtgtatta
     1081 gtcttgatag agaggctgca aatagcagga aacgtgagca gagactccct ggtgtctgaa
     1141 acacaggcca gatgggccct cgagcaggaa acagctatga ccatgattac gccaagctat
     1201 caactttgta tagaaaagtt gctggatccc atcgattcga attcccactg agccaccatg
     1261 tagcctatgt tttatgttca atccacttaa atttgtaaat atgtgtaagt taactgattt
     1321 gtgttgggac aacaggaagg aattatgtgg aacccaacat gttttaacag ttgttcaatt
     1381 tacatgtaga aattagttcc aagaccaatg cttgaacaca actaggtttt agaaactgac
     1441 ccaattaaaa aaggacaact ttgtttcaat tgtgcttgtg ttaaagctgc cttacaaaga
     1501 tgtacagggt aataattaaa gagaaagttc tttattgtgg aatatcttca atataaacgg
     1561 tatcaagcca aaatgtgggg gggccctggt gaagtcagtt gttagcactg ttgctaccaa
     1621 gccagtcagt ttctgcgcca agtttacacg ttctctctgt gctcaagtgg gtttccccca
     1681 ggttccccag tttcctccta ccatccaaaa acatacacca gagacatgct cctagtaagt
     1741 agttatctct taagagcatt cactatctgt tcattatcta ctacagcagg ggagttctcg
     1801 agatttacct gagcccaaac tctcctttcg ctttgcaaat gggagagagc cccggactcg
     1861 aggatcttat gagctcaggg ctctctcccg ggaccgcatg ccaaacaagc ttgaagtaag
     1921 aatgattcta agggcctacg ccgttttagg gcccccagag ataggatgag gggcccatca
     1981 caaccccccc cccctcttca tttttatccg caacacccat gaaatgtttt cttagggcct
     2041 gtaccggcca gcggcgcccc tgtctggata aaaaatgctt ttctgacttg attttttatt
     2101 ttttttgtct tgtgtttgtc tagttcaaaa atggtagcat ggtgactcat tggttcgcac
     2161 tgttgcctaa cagcaagaag atcgctggtt taagtcccgg ctggtccgtt tttgtgtgga
     2221 gattgcatgg tcgtgtgggt ttcctccagg tgcttcgatt tcccccacat tccacagtca
     2281 tgcactatag gtggattgca tgaactaaac tagctgtagt gtatgagagt gtgtgtgaat
     2341 gatagtgtgt atacgtgttt cctagtactg ggttgctgtg gcaatcccag attaacaaaa
     2401 ggatgaataa ttagccaata atttattagc ttgagggaca tgatagcatt gaaactgaac
     2461 atatatcgac tgataactaa tttagccacc aatatattgt ttataaccgc caaaaggttt
     2521 aacagaagtg cctggattgc agacatggca aacgctttgg acgttatctc ccagaacact
     2581 gatttgctgt aactgcagcc taaattaaga catttccagc acctctttaa aataactgtc
     2641 cttttccaga gcacgtcctg aattcccaag taatggccct tttagaatca gttggtgtta
     2701 gcaactcttt cactccatgc tttaaataat acacagtcta aattggcacg gacataaaag
     2761 tcacattatg aaacaaaaag cagcttaatt ttgcagatta ttatttggtt tcactaggtt
     2821 aatacaccac acaagagttc tcaactccgg ccccagagac cccctttcat gcagagttca
     2881 gctccataat caaacacacc tgaaccaact aatccatgtg tcaatacaca cactagcagt
     2941 cttaggagtt ttctcactaa attgacactt tcactaatat actgctatta tttgagtcta
     3001 aatgtatccc tgtatttatt ttcaggacct cacatgtgct aaacttgggt aacacttttt
     3061 attttgatgg tccacttgag tattagtaga ctgtctgctt agcaactgtt gatactgctc
     3121 cttcaacaga catttaactg actataagaa actttgcaag tacagttgaa gtcagaatat
     3181 cccccccccc cctgtttatt ttccccccaa tttctgtgaa cggacagctg atttttttca
     3241 acacattttt aaatgtgaaa aaatattatt agaaatactt tgaaaatttc tgcattgatc
     3301 ctgagggcca gcctgaacac ccactagtga cctacttaca cctgctgctt ctccacgata
     3361 gacgtccaat gttctccagc ctcctgtgcc tagactgcag gtctgcacaa gatgtttggc
     3421 cagaggagaa attgtcgtgc ccaactgagt ctggtttctc tcaaggtctt ttccttcact
     3481 tttgtcaatt ggtgaagtta tgtttctcgc cactgtcccc actggcttgc ttccctgttg
     3541 aaaaatccag cttaaaccag cctaagctgg tttaagctgg atttttcagc agggttggtt
     3601 tgggacttgt ggagctgcgc aatgatggat ttgttcttca ttgtttggac tttcagcagt
     3661 gaaaaattaa accacactga actgaattaa actgaacttc aactctaaaa actggactga
     3721 cggtttcaat tcactagaac ttctatgtta ggctgctttg acccaatcat tgtaaaagcg
     3781 ttaaagaaaa aaaaattaaa ttgaaagcta atatgttctt gataaaatgt ccctaaatag
     3841 acataaacat gtttagagac agtttattta atttttatta ttatttaaat ttaagtaata
     3901 ttatttgaaa aatgatcact tttatatcag aggcaatcat ctatatgccg acgaagatta
     3961 ttgttgacta tttcatattt aaacagtggt tttctttttt catattagcc tactgaagta
     4021 cacagtactt actcatattt agtagctatg ttcacattga aaatcccgtc ccacttaatt
     4081 gaccataagc tggcaataat tagcgtgatt ccacagtgga ccgccacccc gcgctgtcct
     4141 tggtcgcggt gtcggtgggg aaatcctgct caccctcggc tgggatcttt ctcaccccgc
     4201 ctcctgtaat acgatcagct gaagggctaa cagaccccac gcgcctcttc attcactttt
     4261 tctgctccac tttaattcat ccagctaaac gcaaggaccg agcgcggagg agcctctaga
     4321 accggtggta ccgcaagttt gtacaaaaaa gcaggcttaa tggtgagcaa gggcgaggag
     4381 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     4441 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     4501 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
     4561 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     4621 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     4681 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     4741 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     4801 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
     4861 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     4921 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
     4981 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     5041 gccgggatca ctctcggcat ggacgagctg tacaagggag gaggaagatc taagctgaac
     5101 cctcctgatg agagtggccc cggctgcatg agctgcaagt gtgtgctctc ctgaaaccca
     5161 gctttcttgt acaaagtggc tattaagtct cagccaccgt taactgaaca tgtcaaaacc
     5221 tgtggagact gttgagattt gatgttctga aaagataaag cctataaata aaatgttgcc
     5281 caaatttcct gcctgatgtt tttctttgtc tttgctacat ggctttgctg ctcggatcgg
     5341 ctcactctgt gtatgccacg ttcactttgt actctccttc tcacggtagg tttattattt
     5401 ttagatgtgc agttagtttc tgtgaaataa cacaccacac actgatattg tctgtgcatt
     5461 gacttggtga gtgcacattg tttttgatct tgacatattt atatttgatt gatcaggtga
     5521 actgtgtgaa tctaaagtgc tccatacaga tgttctgcat tgaaaatatt ctcattttat
     5581 tagtggaagt gagtgtatgc cagcaacttt attatacata gttgataatt cactggccgt
     5641 cgttttacgg taccatcgat gatgatccag acatgataag atacattgat gagtttggac
     5701 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
     5761 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
     5821 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
     5881 aatgtggtat ggctgattat gatcctctag atcagatctc ttgtttattg cagcttataa
     5941 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     6001 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tctacgtaat
     6061 acgactcact atagttctag aggctcgaga ggggccgctt tacttgtaca gctcgtccat
     6121 gccgagagtg atcccggcgg cggtcacgaa ctccagcagg accatgtgat cgcgcttctc
     6181 gttggggtct ttgctcaggg cggactgggt gctcaggtag tggttgtcgg gcagcagcac
     6241 ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg gcgagctgca cgctgccgtc
     6301 ctcgatgttg tggcggatct tgaagttcac cttgatgccg ttcttctgct tgtcggccat
     6361 gatatagacg ttgtggctgt tgtagttgta ctccagcttg tgccccagga tgttgccgtc
     6421 ctccttgaag tcgatgccct tcagctcgat gcggttcacc agggtgtcgc cctcgaactt
     6481 cacctcggcg cgggtcttgt agttgccgtc gtccttgaag aagatggtgc gctcctggac
     6541 gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg
     6601 gctgaagcac tgcacgccgt aggtcagggt ggtcacgagg gtgggccagg gcacgggcag
     6661 cttgccggtg gtgcagatga acttcagggt cagcttgccg taggtggcat cgccctcgcc
     6721 ctcgccggac acgctgaact tgtggccgtt tacgtcgccg tccagctcga ccaggatggg
     6781 caccaccccg gtgaacagct cctcgccctt gctcaccatg gtcactgtct gctttgctgt
     6841 tggtctgggc tcctgggtca ctggcttact aatggagtct ttatgtatga ggactcttat
     6901 caattgttct tctataaagg tctgcagtgt ttctgttcgt cccctacatg gacacccaga
     6961 gcctcctaaa tacaggagcc ctgataactg cacaagtgct cagattccag cagggtggaa
     7021 aatgagataa agtgtgcaga tggggagggg gacgtgaatg agagatttga gggatgaaaa
     7081 ggatggatga acgcattgaa aatagcccct ttcacaaaat aataccagta aattgtcata
     7141 taattaatga ccagaccttt acctgtaaat gatgtgatgt tcatatacag aagaatgcca
     7201 acaatttaca gatgatttta caaattctta attttccttt tctaaatgga tttgttcaca
     7261 cattatctct tcaaagcaat tgaattttct ggaaaagatt ggctgtgtgt gtaaaaggtg
     7321 ctactgagga atttaatgtg acatggaatg aagcaaacag cagcctaaga caggatggca
     7381 ggaaaaatgt cacatgttta aaataagagt gaaaccaacg acctggactt atagagactg
     7441 tgtgtcctta ggcaaagctc ttgtacaaac agtgcttgtg actgtgaaat tcggggtttg
     7501 cctggattgt gttaaatgtg tgcttggatg tatcataaaa aagaatcggt tctctgtatt
     7561 tgacattttc tgtaatttat ctaaagttac aactggctat gcctgatttt tatcactatt
     7621 gaatcaatca ttttaaagaa tgttaagtct gctgatttgg agagctcatt tacatttatt
     7681 ttgaatgtct cttatttaac acaactgatt taagctttag atctgcgaag atacggccac
     7741 gggtgctctt gatcctgtgg ctgattttgg actgtgctgc tcgcagctgc tgatgaatca
     7801 catacttcct ccattttctt ccactgattg actgttataa tttccctaat ttccaggtca
     7861 aggtgctgtg cattgtggta atagatgtga catgacgtca cttccaaagg accaatgaac
     7921 atgtctgacc aatttcatat aatgtgaaaa cgattttcat aggcagaata aataacattt
     7981 aaattaaact gggcatcagc gcaattcaat tggtttggta atagcaaggg aaaatagaat
     8041 gaagtgatct ccaaaaaata agtacttttt gactgtaaat aaaattgtaa ggagtaaaaa
     8101 gtactttttt ttctaaaaaa atgtaattaa gtaaaagtaa aagtattgat ttttaattgt
     8161 actcaagtaa agtaaaaatc cccaaaaata atacttaagt acagtaatca agtaaaatta
     8221 ctcaagtact ttacacctct ggttcttgac cccctacctt cagcaagccc agcagatcca
     8281 ctagttctag agcggccgcc accgcggtgg agctccagct tttgttccct ttagtgaggg
     8341 ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     8401 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     8461 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     8521 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     8581 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     8641 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     8701 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     8761 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     8821 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     8881 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     8941 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     9001 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     9061 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     9121 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     9181 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     9241 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     9301 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     9361 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggc
     9421 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     9481 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     9541 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     9601 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     9661 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     9721 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     9781 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     9841 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     9901 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     9961 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
    10021 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
    10081 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
    10141 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
    10201 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
    10261 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
    10321 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
    10381 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
    10441 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
    10501 ccccgaaaag tg
//
