LOCUS       sequence-391307-        5755 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pDEL61; Gateway 5' Entry clone encoding nrg1 type II upstream 
            regulatory sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5755)
  AUTHORS   Lysko DE, Talbot WS
  TITLE     Unmyelinated sensory neurons use Neuregulin signals to promote 
            myelination of interneurons in the CNS.
  JOURNAL   Cell Rep. 2022 Nov 15;41(7):111669. doi: 
            10.1016/j.celrep.2022.111669.
  PUBMED    36384112
REFERENCE   2  (bases 1 to 5755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2022-11-15"; volume: "41(7):111669. doi"; pages: " 
            10.1016/j.celrep.2022.111669"
COMMENT     Sequence Label: p5E_(-3kb)nrg1 type II
FEATURES             Location/Qualifiers
     source          1..5755
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    593..688
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    3801..3924
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3996..4015)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3997..4015)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4020..4036)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4020..4036)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4138..4157)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             4149..4958
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(4216..4235)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      5105..5693
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5594..5613
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccga gttaacgcta ccatggagct ccaaataatg
      601 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca atgctttttt
      661 ataatgccaa ctttgtatag aaaagttgct ggatcccatc gattcgaatt cccactgagc
      721 caccatgtag cctatgtttt atgttcaatc cacttaaatt tgtaaatatg tgtaagttaa
      781 ctgatttgtg ttgggacaac aggaaggaat tatgtggaac ccaacatgtt ttaacagttg
      841 ttcaatttac atgtagaaat tagttccaag accaatgctt gaacacaact aggttttaga
      901 aactgaccca attaaaaaag gacaactttg tttcaattgt gcttgtgtta aagctgcctt
      961 acaaagatgt acagggtaat aattaaagag aaagttcttt attgtggaat atcttcaata
     1021 taaacggtat caagccaaaa tgtggggggg ccctggtgaa gtcagttgtt agcactgttg
     1081 ctaccaagcc agtcagtttc tgcgccaagt ttacacgttc tctctgtgct caagtgggtt
     1141 tcccccaggt tccccagttt cctcctacca tccaaaaaca tacaccagag acatgctcct
     1201 agtaagtagt tatctcttaa gagcattcac tatctgttca ttatctacta cagcagggga
     1261 gttctcgaga tttacctgag cccaaactct cctttcgctt tgcaaatggg agagagcccc
     1321 ggactcgagg atcttatgag ctcagggctc tctcccggga ccgcatgcca aacaagcttg
     1381 aagtaagaat gattctaagg gcctacgccg ttttagggcc cccagagata ggatgagggg
     1441 cccatcacaa nccccccccc ctcttcattt ttatccgcaa cacccatgaa atgttttctt
     1501 agggcctgta ccggccagcg gcgcccctgt ctggataaaa aatgcttttc tgacttgatt
     1561 ttttattttt tttgtcttgt gtttgtctag ttcaaaaatg gtagcatggt gactcattgg
     1621 ttcgcactgt tgcctaacag caagaagatc gctggtttaa gtcccggctg gtccgttttt
     1681 gtgtggagat tgcatggtcg tgtgggtttc ctccaggtgc ttcgatttcc cccacattcc
     1741 acagtcatgc actataggtg gattgcatga actaaactag ctgtagtgta tgagagtgtg
     1801 tgtgaatgat agtgtgtata cgtgtttcct agtactgggt tgctgtggca atcccagatt
     1861 aacaaaagga tgaataatta gccaataatt tattagcttg agggacatga tagcattgaa
     1921 actgaacata tatcgactga taactaattt agccaccaat atattgttta taaccgccaa
     1981 aaggtttaac agaagtgcct ggattgcaga catggcaaac gctttggacg ttatctccca
     2041 gaacactgat ttgctgtaac tgcagcctaa attaagacat ttccagcacc tctttaaaat
     2101 aactgtcctt ttccagagca cgtcctgaat tcccaagtaa tggccctttt agaatcagtt
     2161 ggtgttagca actctttcac tccatgcttt aaataataca cagtctaaat tggcacggac
     2221 ataaaagtca cattatgaaa caaaaagcag cttaattttg cagattatta tttggtttca
     2281 ctaggttaat acaccacaca agagttctca actccggccc cagagacccc ctttcatgca
     2341 gagttcagct ccataatcaa acacacctga accaactaat ccatgtgtca atacacacac
     2401 tagcagtctt aggagttttc tcactaaatt gacactttca ctaatatact gctattattt
     2461 gagtctaaat gtatccctgt atttattttc aggacctcac atgtgctaaa cttgggtaac
     2521 actttttatt ttgatggtcc acttgagtat tagtagactg tctgcttagc aactgttgat
     2581 actgctcctt caacagacat ttaactgact ataagaaact ttgcaagtac agttgaagtc
     2641 agaatatccc ccccccccct gtttattttc cccccaattt ctgtgaacgg acagctgatt
     2701 tttttcaaca catttttaaa tgtgaaaaaa tattattaga aatactttga aaatttctgc
     2761 attgatcctg agggccagcc tgaacaccca ctagtgacct acttacacct gctgcttctc
     2821 cacgatagac gtccaatgtt ctccagcctc ctgtgcctag actgcaggtc tgcacaagat
     2881 gtttggccag aggagaaatt gtcgtgccca actgagtctg gtttctctca aggtcttttc
     2941 cttcactttt gtcaattggt gaagttatgt ttctcgccac tgtccccact ggcttgcttc
     3001 cctgttgaaa aatccagctt aaaccagcct aagctggttt aagctggatt tttcagcagg
     3061 gttggtttgg gacttgtgga gctgcgcaat gatggatttg ttcttcattg tttggacttt
     3121 cagcagtgaa aaattaaacc acactgaact gaattaaact gaacttcaac tctaaaaact
     3181 ggactgacgg tttcaattca ctagaacttc tatgttaggc tgctttgacc caatcattgt
     3241 aaaagcgtta aagaaaaaaa aattaaattg aaagctaata tgttcttgat aaaatgtccc
     3301 taaatagaca taaacatgtt tagagacagt ttatttaatt tttattatta tttaaattta
     3361 agtaatatta tttgaaaaat gatcactttt atatcagagg caatcatcta tatgccgacg
     3421 aagattattg ttgactattt catatttaaa cagtggtttt cttttttcat attagcctac
     3481 tgaagtacac agtacttact catatttagt agctatgttc acattgaaaa tcccgtccca
     3541 cttaattgac cataagctgg caataattag cgtgattcca cagtggaccg ccaccccgcg
     3601 ctgtccttgg tcgcggtgtc ggtggggaaa tcctgctcac cctcggctgg gatctttctc
     3661 accccgcctc ctgtaatacg atcagctgaa gggctaacag accccacgcg cctcttcatt
     3721 cactttttct gctccacttt aattcatcca gctaaacgca aggaccgagc gcggaggagc
     3781 ctctagaacc ggtggtaccg caagtttgta caaaaaagtt gaacgagaaa cgtaaaatga
     3841 tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat aatactgtaa
     3901 aacacaacat atgcagtcac tatgaaccaa ctacttagat ggtattagtg acctgtagaa
     3961 ttcgagctct agagctgcag ggcggccgcg atatccccta tagtgagtcg tattacatgg
     4021 tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac
     4081 aagataaaaa tatatcatca tgaacaataa aactgtctgc ttacataaac agtaatacaa
     4141 ggggtgttat gagccatatt caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg
     4201 atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa
     4261 tctatcgctt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta
     4321 gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc
     4381 ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg
     4441 cgatccccgg aaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata
     4501 ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc
     4561 cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt
     4621 tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga
     4681 aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat ggtgatttct
     4741 cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag
     4801 tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt
     4861 ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata
     4921 aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt aattggttgt
     4981 aacactggca gagcattacg ctgacttgac gggacggcgc aagctcatga ccaaaatccc
     5041 ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     5101 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     5161 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     5221 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     5281 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     5341 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     5401 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     5461 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     5521 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     5581 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     5641 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     5701 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgtt
//
