LOCUS       sequence-39131-e         808 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of the reporter gene under the control of the 
            MiniPromoter in this plasmid has not been tested.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Portales-Casamar E, Swanson DJ, Liu L, de Leeuw CN, Banks KG, Ho Sui
            SJ, Fulton DL, Ali J, Amirabbasi M, Arenillas DJ, Babyak N, Black 
            SF, Bonaguro RJ, Brauer E, Candido TR, Castellarin M, Chen J, Chen 
            Y, Cheng JC, Chopra V, Docking TR, Dreolini L, D'Souza CA, Flynn EK,
            Glenn R, Hatakka K, Hearty TG, Imanian B, Jiang S, Khorasan-zadeh S,
            Komljenovic I, Laprise S, Liao NY, Lim JS, Lithwick S, Liu F, Liu J,
            Lu M, McConechy M, McLeod AJ, Milisavljevic M, Mis J, O'Connor K, 
            Palma B, Palmquist DL, Schmouth JF, Swanson MI, Tam B, Ticoll A, 
            Turner JL, Varhol R, Vermeulen J, Watkins RF, Wilson G, Wong BK, 
            Wong SH, Wong TY, Yang GS, Ypsilanti AR, Jones SJ, Holt RA, 
            Goldowitz D, Wasserman WW, Simpson EM
  TITLE     A regulatory toolbox of MiniPromoters to drive selective expression 
            in the brain.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Sep 21. 107(38):16589-94.
  PUBMED    20807748
REFERENCE   2  (bases 1 to 808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2010 Sep 21. 107(38):16589-94."
COMMENT     Sequence Label: pEMS1613
FEATURES             Location/Qualifiers
     source          1..808
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     153..175
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     regulatory      186..195
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     intron          245..377
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
ORIGIN
        1 ttctggtgcc ggaaaccagg caaagcgcca ttcgccattc aggctgcgca actgttggga
       61 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
      121 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggg
      181 atccgccatg gtggcggaag ttcctatacc ttttgaagaa taggaacttc ggaataggaa
      241 cttcctgtgg agagaaaggc aaagtggatg tcagtaagac caataggtgc ctatcagaaa
      301 cgcaagagtc ttctctgtct cgacaagccc agtttctatt ggtctcctta aacctgtctt
      361 gtaaccttga tacttacggc gcgccgcgga gcggttggtg ggcgctgccc gagtcccggc
      421 cggcagcgcc cgcctcgccg ccgggcgccg ccgctctcca gctgccctcc agcctgcaag
      481 gctcctgggc gggacaggtg ggtgtcagtc gcgcgctgcg ctgctccctg ggcgctcaga
      541 attcgtccaa cagtggaaat ttaagcatcc ttaagtttct tcccggctct tgcaaaaccg
      601 cagctgctgg gaatgctgct tgatatggac acacacatgg acagacagat ggagagatgg
      661 acaggaagac tgaggaagaa ggaagaacag caagagaggg atgttgaaag acagagatgg
      721 agacacgaaa atggccttcc tacggagcga aatccctctt tttccccatt cctgtcactc
      781 agtcttcgat ctctccctct acataggc
//
