LOCUS       sequence-391337-        9361 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses VAMP2, fused to mCherry, in mammalian cells. Can be used 
            in generating stable cell lines via lentiviral transduction..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9361)
  AUTHORS   Roh H, Dorner BG, Ting AY
  TITLE     Cell-Type-Specific Intracellular Protein Delivery with Inactivated 
            Botulinum Neurotoxin.
  JOURNAL   J Am Chem Soc. 2023 May 10;145(18):10220-10226. doi: 
            10.1021/jacs.3c01145. Epub 2023 Apr 26.
  PUBMED    37098770
REFERENCE   2  (bases 1 to 9361)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.3c01145"; journalName: "J Am Chem Soc"; date: 
            "2023-05-10- 10"; volume: "145"; issue: "18"; pages: "10220-10226"
COMMENT     Sequence Label: pLX208_CMV::mCherry-myc-VAMP2
FEATURES             Location/Qualifiers
     source          1..9361
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1058..1284
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1285..1465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1512..1637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2130..2363
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2548..2592
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2767..3267
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3186..3204
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             3279..4298
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     "
     misc_feature    4355..4472
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        4589..4892
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4893..5096
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5047..5067
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      5119..5128
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5134..5838
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(5276..5294)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5544..5563
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     5738..5761
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             5857..5886
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_feature    6271..6859
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6324..6344)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6742..6753)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6931..7164
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7242..7363
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7279..7298)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7333..7352
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7403..7538
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7558..7577)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7559..7577)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7587..7604)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7587..7603)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7596..7618)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7745..8200
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7832..7851)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8042..8063
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8226..8330
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8331..8399,8400..9191)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8549..8568)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     1081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     1141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     1201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     1261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     2041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     2101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     2161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     2221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     2281 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     2341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     2401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     2461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     2521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     2581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2701 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2761 ggacccttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg
     2821 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt
     2881 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct
     2941 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa
     3001 gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc
     3061 cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc
     3121 cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc
     3181 gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt
     3241 gaccgaatca ccgacctctc tccccagggg gtaccaccat gaaaaagcct gaactcaccg
     3301 ctacctctgt cgagaagttt ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc
     3361 tctccgaggg cgaagaatct cgggctttca gcttcgatgt gggagggcgt ggatatgtcc
     3421 tgcgggtgaa tagctgcgcc gatggtttct acaaagatcg ctatgtttat cggcactttg
     3481 catccgccgc tctccctatt cccgaagtgc ttgacattgg ggagttcagc gagagcctga
     3541 cctattgcat ctcccgccgt gcacagggtg tcaccttgca agacctgcct gaaaccgaac
     3601 tgcccgctgt tctccagccc gtcgccgagg ccatggatgc catcgctgcc gccgatctta
     3661 gccagaccag cgggttcggc ccattcggac ctcaaggaat cggtcaatac actacatggc
     3721 gcgatttcat ctgcgctatt gctgatcccc atgtgtatca ctggcaaact gtgatggacg
     3781 acaccgtcag tgcctccgtc gcccaggctc tcgatgagct gatgctttgg gccgaggact
     3841 gccccgaagt ccggcacctc gtgcacgccg atttcggctc caacaatgtc ctgaccgaca
     3901 atggccgcat aacagccgtc attgactgga gcgaggccat gttcggggat tcccaatacg
     3961 aggtcgccaa catcttcttc tggaggccct ggttggcttg tatggagcag cagacccgct
     4021 acttcgagcg gaggcatccc gagcttgcag gatctcctcg gctccgggct tatatgctcc
     4081 gcattggtct tgaccaactc tatcagagct tggttgacgg caatttcgat gatgcagctt
     4141 gggctcaggg tcgctgcgac gcaatcgtcc ggtccggagc cgggactgtc gggcgtacac
     4201 aaatcgcccg cagaagcgct gccgtctgga ccgatggctg tgtggaagtg ctcgccgata
     4261 gtggaaacag acgccccagc actcgtccta gggcaaagga tctgcagtaa tgagaattct
     4321 agatcttgag acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
     4381 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
     4441 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
     4501 cactttggcg ccggctcgag ggggcccggg gttattaata gtaatcaatt acggggtcat
     4561 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
     4621 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
     4681 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
     4741 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
     4801 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     4861 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
     4921 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     4981 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     5041 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctctct
     5101 ggctaactgt cgggatccgc caccatggct agcgtgagca agggcgagga ggacaacatg
     5161 gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac
     5221 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag
     5281 ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc
     5341 atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg
     5401 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg
     5461 accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc
     5521 ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc
     5581 tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg
     5641 aagctgaagg acggcggcca ctacgacgcc gaggtcaaga ccacctacaa ggccaagaag
     5701 cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagc tggacatcac ctcccacaac
     5761 gaggactaca ccatcgtgga acagtacgag cgcgccgagg gccgccactc caccggcggc
     5821 atggacgagc tgtacaaggc tagcgttaac accggtgaac aaaagcttat ttctgaagag
     5881 gacttgaccg gtcttaagtc ggctaccgct gccaccgtcc cgcctgccgc cccggccggc
     5941 gagggtggcc cccctgcacc tcctccaaac cttactagta acaggagact gcagcagacc
     6001 caggcccagg tggatgaggt ggtggacatc atgagggtga atgtggacaa ggtcctggag
     6061 cgggaccaga agttgtcgga gctggatgac cgtgcagatg ccctccaggc aggggcctcc
     6121 cagtttgaaa caagtgcagc caagctcaag cgcaaatact ggtggaaaaa cctcaagatg
     6181 atgatcatct tgggagtgat ctgcgccatc atcctcatca tcatcatcgt ttacttcagc
     6241 acttaactta agtaaacgcg ttaagtcgac aatcaacctc tggattacaa aatttgtgaa
     6301 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     6361 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     6421 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     6481 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     6541 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     6601 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     6661 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     6721 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     6781 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     6841 ctttgggccg cctccccgcg tcgactttaa gaccaatgac ttacaaggca gctgtagatc
     6901 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     6961 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     7021 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     7081 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     7141 agtcagtgtg gaaaatctct agcagtacgt atagtagttc atgtcatctt attattcagt
     7201 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     7261 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     7321 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     7381 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     7441 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     7501 gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc
     7561 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
     7621 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     7681 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     7741 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     7801 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     7861 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     7921 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     7981 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     8041 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     8101 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     8161 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
     8221 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     8281 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     8341 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     8401 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     8461 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     8521 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     8581 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     8641 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     8701 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     8761 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     8821 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     8881 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     8941 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     9001 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     9061 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     9121 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     9181 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     9241 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     9301 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     9361 c
//
