LOCUS       sequence-391365-        4236 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick (MoClo) Level 0 Part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4236)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 4236)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pMC-1-PvUbi1-6
FEATURES             Location/Qualifiers
     source          1..4236
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(798..809)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2116..2134)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2202..2306
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2528..3319
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3490..4078
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3979..3998
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(4232..4236,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcag gagcacgtca gtgtttggtt tccactagca
      121 cgagtagcgc aatcagaaaa ttttcaatgc atgaagtact aaacgaagtt tatttagaaa
      181 tttttttaag aaatgagtgt aattttttgc gacgaattta atgacaataa ttaatcgatg
      241 attgcctaca gtaatgctac agtaaccaac ctctaatcat gcgtcgaatg cgtcattaga
      301 ttcgtctcgc aaaatagcac aagaattatg aaattaattt tacaaactat ttttatttaa
      361 tactaataat taactgtcaa agtttgtgct actcgcaaga gtagcgcgaa ccaaacacgg
      421 cctggaggag cacggtaacg gcgtcgacaa actaacggcc accacccgcc aacgcaaagg
      481 agacggatga gagttgactt cttgacggtt ctccacccct ctgtctctct gtcactgggc
      541 cctgggtccc cctctcgaaa gttcctctgg ccgaaattgc gcggcggaga cgaggcgggc
      601 ggaaccgtca cggcagagga ttccttcccc accctgcctg gcccggccat atataaacag
      661 ccaccgcccc tccccgttcc ccatcgcgtc tcgtctcgtg ttgttcccag aacacaacca
      721 aaatccaaat cctcctcctc ctcccgagcc tcgtcgatcc ctcacccgct tcaaggtacg
      781 gcgatcctcc tctcccttct cccctcgatc gattatgcgt gttccgtttc cgtttccgat
      841 cgagcgaatc gatggttagg acccatgggg gacccatggg gtgtcgtgtg gtggtctggt
      901 ttgatccgcg atatttctcc gttcgtagtg tagatctgat cgaatccctg gtgaaatcgt
      961 tgatcgtgct attcgtgtga gggttcttag gtttggagtt gtggaggtag ttctgatcgg
     1021 tttgtaggtg agattttccc catgattttg cttggctcgt ttgtcttggt tagattagat
     1081 ctgcccgcat tttgttcgat atttctgatg cagatatgat gaataatttc gtccttgtat
     1141 cccgcgtccg tatgtgtatt aagtttgcag gtcctagtta ggtttttcct actgatttgt
     1201 cttatccatt ctgtttagct tgcaaggttt ggtaatggtc cggcatgttt gtctctatag
     1261 attagagtag aataagatta tctcaacaag ctgttggctt atcaattttg gatctgcatg
     1321 tgtttcgcat ctatatcttt gcaattaaga tggtagatgg acatatgctc ctgttgagtt
     1381 gatgttgtac cttttacctg aggtctgagg aacatgcatc ctcctgctac tttgtgctta
     1441 tacagatcat caagattatg tagctaatat tcgatcagtt tctagtatct acatggtaaa
     1501 cttgcatgca cttgctactt atttttgata tacttggatg ataacatatg ctgctggttg
     1561 attcctacct acatgatgaa cattttacag gccattagtg tctgtctgta tgtgttgttc
     1621 ctgtttgctt cagtctattt ctgtttcatt cctagtttat tggttctctg ctagatactt
     1681 accctgctgg gcttagttat catcttatct cgaatgcatt ttcatgttta tagatgaata
     1741 tacactcaga taggtgtaga tgtatgctac tgtttctcta cgttgctgta ggttttacct
     1801 gtggcaactg catactcctg ttgcttcgct agatatgtat gtgcttatat agattaagat
     1861 atgtgtgatg gttctttagt atatctgatg atcatgtatg ctcttttaac ttcttgctac
     1921 acttggtaac atgctgtgat gctgtttgtt gattctgtag cactaccaat gatgacctta
     1981 tctctctttg tatatgatgt ttctgtttgt ttgaggcttg tgttactgct agttacttac
     2041 cctgttgcct ggctaatctt ctgcagatcc tgcaggaatg tgagaccacg aagtggctct
     2101 tcagtggacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     2161 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     2221 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     2281 ttcaataata ttgaaaaagg aagagtatgc gctcacgcaa ctggtccaga accttgaccg
     2341 aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttt
     2401 tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg
     2461 tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt
     2521 aaacatcatg agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg
     2581 cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt
     2641 ggatggcggc ctgaagccac acagcgatat tgatttgctg gttacggtga ccgtaaggct
     2701 tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg
     2761 agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc
     2821 gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct
     2881 tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc
     2941 aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc
     3001 tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga
     3061 ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt
     3121 aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc
     3181 ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg
     3241 cttggcctcg cgcgcagatc agttggaaga atttgtccat tacgtaaaag gcgagatcac
     3301 caaggtagtc ggcaaataac tgtcagacca agtttactca tatatacttt agattgattt
     3361 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     3421 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     3481 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     3541 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     3601 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     3661 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     3721 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     3781 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     3841 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     3901 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     3961 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     4021 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     4081 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     4141 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     4201 taccgctcgc cgcagccgaa cgaccgagcg cagcga
//
