LOCUS       sequence-391366-        4269 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick (MoClo) Level 0 Part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 4269)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pMC-1-ZmUbi1-6
FEATURES             Location/Qualifiers
     source          1..4269
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2149..2167)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2235..2339
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2561..3352
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3523..4111
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4012..4031
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(4265..4269,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcag gagggcgcgc ctgcagtgca gcgtgacccg
      121 gtcgtgcccc tctctagaga taatgagcat tgcatgtcta agttataaaa aattaccaca
      181 tatttttttt gtcacacttg tttgaagtgc agtttatcta tctttataca tatatttaaa
      241 ctttactcta cgaataatat aatctatagt actacaataa tatcagtgtt ttagagaatc
      301 atataaatga acagttagac atggtctaaa ggacaattga gtattttgac aacaggactc
      361 tacagtttta tctttttagt gtgcatgtgt tctccttttt ttttgcaaat agcttcacct
      421 atataatact tcatccattt tattagtaca tccatttagg gtttagggtt aatggttttt
      481 atagactaat ttttttagta catctatttt attctatttt agcctctaaa ttaagaaaac
      541 taaaactcta ttttagtttt tttatttaat aatttagata taaaatagaa taaaataaag
      601 tgactaaaaa ttaaacaaat accctttaag aaattaaaaa aactaaggaa acatttttct
      661 tgtttcgagt agataatgcc agcctgttaa acgccgtcga cgagtctaac ggacaccaac
      721 cagcgaacca gcagcgtcgc gtcgggccaa gcgaagcaga cggcacggca tctctgtcgc
      781 tgcctctgga cccctctcga gagttccgct ccaccgttgg acttgctccg ctgtcggcat
      841 ccagaaattg cgtggcggag cggcagacgt gagccggcac ggcaggcggc ctcctcctcc
      901 tctcacggca ccggcagcta cgggggattc ctttcccacc gctccttcgc tttcccttcc
      961 tcgcccgccg taataaatag acaccccctc cacaccctct ttccccaacc tcgtgttgtt
     1021 cggagcgcac acacacacaa ccagatctcc cccaaatcca cccgtcggca cctccgcttc
     1081 aaggtacgcc gctcgtcctc cccccccccc tctctacctt ctctagatcg gcgttccggt
     1141 ccatggttag ggcccggtag ttctacttct gttcatgttt gtgttagatc cgtgtttgtg
     1201 ttagatccgt gctgctagcg ttcgtacacg gatgcgacct gtacgtcaga cacgttctga
     1261 ttgctaactt gccagtgttt ctctttgggg aatcctggga tggctctagc cgttccgcag
     1321 acgggatcga tttcatgatt ttttttgttt cgttgcatag ggtttggttt gcccttttcc
     1381 tttatttcaa tatatgccgt gcacttgttt gtcgggtcat cttttcatgc ttttttttgt
     1441 cttggttgtg atgatgtggt ctggttgggc ggtcgttcta gatcggagta gaattaattc
     1501 tgtttcaaac tacctggtgg atttattaat tttggatctg tatgtgtgtg ccatacatat
     1561 tcatagttac gaattgaaga tgatggatgg aaatatcgat ctaggatagg tatacatgtt
     1621 gatgcgggtt ttactgatgc atatacagag atgctttttg ttcgcttggt tgtgatgatg
     1681 tggtgtggtt gggcggtcgt tcattcgttc tagatcggag tagaatactg tttcaaacta
     1741 cctggtgtat ttattaattt tggaactgta tgtgtgtgtc atacatcttc atagttacga
     1801 gtttaagatg gatggaaata tcgatctagg ataggtatac atgttgatgt gggttttact
     1861 gatgcatata catgatggca tatgcagcat ctattcatat gctctaacct tgagtaccta
     1921 tctattataa taaacaagta tgttttataa ttattttgat cttgatatac ttggatgatg
     1981 gcatatgcag cagctatatg tggatttttt tagccctgcc ttcatacgct atttatttgc
     2041 ttggtactgt ttcttttgtc gatgctcacc ctgttgtttg gtgttacttc ctgcagggcg
     2101 atctattcga atgtgagacc acgaagtggc tcttcagtgg acgaaagggc ctcgtgatac
     2161 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     2221 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     2281 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     2341 tgcgctcacg caactggtcc agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg
     2401 cggttttcat ggcttgttat gactgttttt ttttggggta cagtctatgc ctcgggcatc
     2461 caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt
     2521 tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc atgagggaag cggtgatcgc
     2581 cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac
     2641 gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagcga
     2701 tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat
     2761 caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga
     2821 agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact
     2881 gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat
     2941 cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg
     3001 tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa
     3061 tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt
     3121 gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt
     3181 cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc
     3241 tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga
     3301 agaatttgtc cattacgtaa aaggcgagat caccaaggta gtcggcaaat aactgtcaga
     3361 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     3421 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     3481 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     3541 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     3601 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     3661 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     3721 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     3781 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     3841 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     3901 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     3961 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     4021 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     4081 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     4141 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     4201 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     4261 gcgcagcga
//
