LOCUS       sequence-391373-        9945 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  All-in-one lentiviral backbone for doxycycline-inducible expression 
            of a 3xFLAG-tagged protein of interest, which can be inserted with 
            Golden Gate cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9945)
  AUTHORS   Ludwig CH, Thurm AR, Morgens DW, Yang KJ, Tycko J, Bassik MC, 
            Glaunsinger BA, Bintu L
  TITLE     High-throughput discovery and characterization of viral 
            transcriptional effectors in human cells.
  JOURNAL   Cell Syst. 2023 Jun 21;14(6):482-500.e8. doi: 
            10.1016/j.cels.2023.05.008.
  PUBMED    37348463
REFERENCE   2  (bases 1 to 9945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2023.05"; journalName: "Cell Syst"; date: 
            "2023-06-21- 21"; volume: "14"; issue: "6"; pages: "482-500.e8"
COMMENT     Sequence Label: pCL040 lenti TRE-3xFLAG-LibCloneSite
            pEF-rTetR...
FEATURES             Location/Qualifiers
     source          1..9945
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        68..446
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    76..94
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    112..130
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    148..166
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    184..202
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    220..238
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    256..274
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    292..310
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     355..379
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      454..463
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             463..528
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     polyA_signal    613..734
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(650..669)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     704..723
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        1030..1241
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          1242..2180
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2137..2157
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             2953..3006
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3007..3714
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3152..3170)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3420..3439
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             3733..4131
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     protein_bind    complement(4151..4184)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    4240..4828
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4293..4313)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4711..4722)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4831..4847)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4832..4848)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5357..5537
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(5599..6187)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5679..5698)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6358..7149,7150..7218))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6981..7000
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7219..7323)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7398..7417)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7589..7968
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7970..8168
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8123..8143
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8186..8366
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8413..8538
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9035..9268
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9453..9497
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9795..9912
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tggttagtac cgggcccgct ctagagatcc catttgactc aggcggccgc gccactagtc
       61 gagagttggc tttactccct atcagtgata gagaacgtat gaagagttta ctccctatca
      121 gtgatagaga acgtatgcag actttactcc ctatcagtga tagagaacgt ataaggagtt
      181 tactccctat cagtgataga gaacgtatga ccagtttact ccctatcagt gatagagaac
      241 gtatctacag tttactccct atcagtgata gagaacgtat atccagttta ctccctatca
      301 gtgatagaga acgtataagc tttaggcgtg tacggtgggc gcctataaaa gcagagctcg
      361 tttagtgaac cgtcagatcg cctggagcaa ttccacaaca cttttgtctt ataccaactt
      421 tccgtaccac ttcctaccct cgtaaaaccg gtcgccacca tggactacaa agaccatgac
      481 ggtgattata aagatcatga catcgactac aaggatgacg atgacaaggg tggatccgga
      541 tcaggctccg gagacgggtc aagcgtctct ggatctggat aagtttaaac tcgcgaatcg
      601 atcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
      661 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
      721 atcaatgtat cttatgcata agtaacaaaa cttttatgag ggacagcccc cccccaaagc
      781 ccccagggat gtaattacgt ccctcccccg ctagggggca gcagcgagcc gcccggggct
      841 ccgctccggt ccggcgctcc ccccgcatcc ccgagccggc agcgtgcggg gacagcccgg
      901 gcacggggaa ggtggcacgg gatcgctttc ctctgaacgc ttctcgctgc tctttgagcc
      961 tgcagacacc tggggggata cggggaaaag gcctccacgg ccactagtat tatgcatgtg
     1021 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg
     1081 gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt
     1141 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg
     1201 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt
     1261 cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct
     1321 ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga
     1381 ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct
     1441 ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt
     1501 ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc
     1561 ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg
     1621 acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac
     1681 cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc
     1741 cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag
     1801 cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct
     1861 cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg
     1921 tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcgc
     1981 gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc
     2041 acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg
     2101 aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa
     2161 gtttttttct tccatttcag gtgtcgtgag ctagccacca tgtctagact ggacaagagc
     2221 aaaataataa acggagctct ggaattactc aatggtgtcg gtatcgaagg cctgacgaca
     2281 aggaaactcg ctcaaaagct gggagttgag cagcctaccc tgtactggca cgtgaagaac
     2341 aagcgggccc tgctcgatgc cctgccaatc gagatgctgg acaggcatca tacccactcc
     2401 tgccccctgg aacccgagtc atggcaagac tttctgcgga acaacgccaa gtcataccgc
     2461 tgtgctctcc tctcacatcg cgacggggct aaagtgcatc tcggcacccg cccaacagag
     2521 aaacagtacg aaaccctgga aaatcagctc gcgttcctgt gtcagcaagg cttctccctg
     2581 gagaacgcac tgtacgctct gtccgccgtg ggccacttta cactgggctg cgtattggag
     2641 gaacaggagc atcaagtagc aaaagaggaa agagagacac ctaccaccga ttctatgccc
     2701 ccacttctga aacaagcaat tgagctgttc gaccggcagg gagccgaacc tgccttcctt
     2761 ttcggcctgg aactaatcat atgtggcctg gagaaacagc taaagtgcga aagcggcggt
     2821 cccaccgacg ccctggacga cttcgacctg gacatgctgc ccgccgatgc cctggatgat
     2881 tttgatctgg atatgctccc tgctgacgct ctcgatgact tcgatctcga tatgctgcca
     2941 ggcggatctg gagagggaag gggaagcctc ctaacttgcg gagatgtcga ggagaatcct
     3001 ggcccaatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc
     3061 ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc
     3121 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc
     3181 ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg
     3241 aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg
     3301 gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg
     3361 caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc
     3421 cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag
     3481 gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac
     3541 gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac
     3601 aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag
     3661 tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta caagggtagc
     3721 ggcagcggta gcatggccaa gcctttgtct caagaagaat ccaccctcat tgaaagagca
     3781 acggctacaa tcaacagcat ccccatctct gaagactaca gcgtcgccag cgcagctctc
     3841 tctagcgacg gccgcatctt cactggtgtc aatgtatatc attttactgg gggaccttgt
     3901 gcagaactcg tggtgctggg cactgctgct gctgcggcag ctggcaacct gacttgtatc
     3961 gtcgcgatcg gaaatgagaa caggggcatc ttgagcccct gcggacggtg ccgacaggtg
     4021 cttctcgatc tgcatcctgg gatcaaagcc atagtgaagg acagtgatgg acagccgacg
     4081 gcagttggga ttcgtgaatt gctgccctct ggttatgtgt gggagggcta agaattcgtc
     4141 gagggaccta ataacttcgt atagcataca ttatacgaag ttatacatgt ttaagggttc
     4201 cggttccact aggtacaatt cgatatcaag cttatcgata atcaacctct ggattacaaa
     4261 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     4321 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     4381 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     4441 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     4501 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     4561 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     4621 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     4681 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     4741 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     4801 cggatctccc tttgggccgc ctccccgcat cgataccgtc gacctcgatc gagacctaga
     4861 aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct
     4921 agaagcacaa gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc
     4981 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     5041 agggctaatt cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca
     5101 aggctacttc cctgattggc agaactacac accagggcca gggatcagat atccactgac
     5161 ctttggatgg tgctacaagc tagtaccagt tgagcaagag aaggtagaag aagccaatga
     5221 aggagagaac acccgcttgt tacaccctgt gagcctgcat gggatggatg acccggagag
     5281 agaagtatta gagtggaggt ttgacagccg cctagcattt catcacatgg cccgagagct
     5341 gcatccggac tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     5401 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     5461 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     5521 gtggaaaatc tctagcagca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     5581 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     5641 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     5701 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     5761 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     5821 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     5881 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     5941 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     6001 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     6061 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     6121 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     6181 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     6241 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     6301 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     6361 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     6421 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     6481 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     6541 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     6601 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     6661 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     6721 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     6781 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     6841 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     6901 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     6961 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     7021 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     7081 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     7141 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     7201 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     7261 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     7321 gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc
     7381 ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc
     7441 cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg
     7501 caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt
     7561 cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa
     7621 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
     7681 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
     7741 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
     7801 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
     7861 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
     7921 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
     7981 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
     8041 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
     8101 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
     8161 ataagcagcg cgttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga
     8221 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     8281 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     8341 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
     8401 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
     8461 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
     8521 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
     8581 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
     8641 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
     8701 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
     8761 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
     8821 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
     8881 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
     8941 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
     9001 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
     9061 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
     9121 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
     9181 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
     9241 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
     9301 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
     9361 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
     9421 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
     9481 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
     9541 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
     9601 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
     9661 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca
     9721 gatccattcg attagtgaac ggatcggcac tgcgtgcgcc aattctgcag acaaatggca
     9781 gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     9841 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     9901 attcaaaatt ttcgggttta ttacagggac agcagagatc cagtt
//
