LOCUS       sequence-391377-        9661 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Conjugally transferable red recombinogenic plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9661)
  AUTHORS   Hossain MJ, Thurlow CM, Sun D, Nasrin S, Liles MR
  TITLE     Genome modifications and cloning using a conjugally transferable 
            recombineering system.
  JOURNAL   Biotechnol Rep (Amst). 2015 Aug 28;8:24-35. doi: 
            10.1016/j.btre.2015.08.005. eCollection 2015 Dec.
  PUBMED    28352570
REFERENCE   2  (bases 1 to 9661)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Rep (Amst)."; date: "2015-08-28"; volume: "8:24-35. doi"; pages: " 
            10.1016/j.btre.2015.08.005. eCollection 2015 Dec"
COMMENT     Sequence Label: pMJH46
FEATURES             Location/Qualifiers
     source          1..9661
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1..879)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        906..1190
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1113..1132
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     RBS             1217..1225
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             1234..1650
                     /codon_start=1
                     /gene="gam"
                     /product="inhibitor of the host RecBCD nuclease in the 
                     lambda Red system"
                     /label=Gam
                     /note="protects the double-stranded DNA substrate for 
                     recombination"
                     /translation="MDINTETEIKQKHSLTPFPVFLISPAFRGRYFHSYFRSSAMNAYY
                     IQDRLEAQSWARHYQQLAREEKEAELADDMEKGLPQHLFESLCIDHLQRHGASKKSITR
                     AFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     CDS             1656..2441
                     /codon_start=1
                     /gene="bet"
                     /product="single-stranded DNA binding recombinase in the 
                     lambda Red system"
                     /label=Beta
                     /note="mediates pairing of a single-stranded DNA with a 
                     complementary target"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQFI
                     ALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNESC
                     TCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCARLA
                     FGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDDDLLP
                     LCSQIFRRDIRASSELTQAEAVKALGFLKQKAAEQKVAA"
     CDS             2438..3118
                     /codon_start=1
                     /gene="exo"
                     /product="5' to 3' double-stranded DNA exonuclease in the 
                     lambda Red system"
                     /label=Exo
                     /note="creates 3' single-stranded DNA tails"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKPR
                     SGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTESP
                     IIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQYSMW
                     VTRKNAWYFANYDPRMKREGLHYVVIERDEKYMASFDEIVPEFIEKMDEALAEIGFVFG
                     EQWR"
     terminator      3119..3363
                     /label=lambda tL3 terminator
                     /note="transcription terminator tL3 from phage lambda"
     oriT            4226..4335
                     /note="incP origin of transfer"
     CDS             4368..4739
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     promoter        5785..5887
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             5888..6547
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDSRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(5954..5973)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             complement(6819..7769)
                     /codon_start=1
                     /gene="rep101 encoding an A56V mutation"
                     /product="temperature-sensitive version of the RepA protein
                     needed for replication with the pSC101 origin (Armstrong et
                     al., 1984)"
                     /label=Rep101(Ts)
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYVQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      7817..8039
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             complement(8958..9336)
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     9283..9302
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9521..9625)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaag tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggaattc gagctctaag gaggttataa aaaatggata ttaatactga aactgagatc
     1261 aagcaaaagc attcactaac cccctttcct gttttcctaa tcagcccggc atttcgcggg
     1321 cgatattttc acagctattt caggagttca gccatgaacg cttattacat tcaggatcgt
     1381 cttgaggctc agagctgggc gcgtcactac cagcagctcg cccgtgaaga gaaagaggca
     1441 gaactggcag acgacatgga aaaaggcctg ccccagcacc tgtttgaatc gctatgcatc
     1501 gatcatttgc aacgccacgg ggccagcaaa aaatccatta cccgtgcgtt tgatgacgat
     1561 gttgagtttc aggagcgcat ggcagaacac atccggtaca tggttgaaac cattgctcac
     1621 caccaggttg atattgattc agaggtataa aacgaatgag tactgcactc gcaacgctgg
     1681 ctgggaagct ggctgaacgt gtcggcatgg attctgtcga cccacaggaa ctgatcacca
     1741 ctcttcgcca gacggcattt aaaggtgatg ccagcgatgc gcagttcatc gcattactga
     1801 tcgttgccaa ccagtacggc cttaatccgt ggacgaaaga aatttacgcc tttcctgata
     1861 agcagaatgg catcgttccg gtggtgggcg ttgatggctg gtcccgcatc atcaatgaaa
     1921 accagcagtt tgatggcatg gactttgagc aggacaatga atcctgtaca tgccggattt
     1981 accgcaagga ccgtaatcat ccgatctgcg ttaccgaatg gatggatgaa tgccgccgcg
     2041 aaccattcaa aactcgcgaa ggcagagaaa tcacggggcc gtggcagtcg catcccaaac
     2101 ggatgttacg tcataaagcc atgattcagt gtgcccgtct ggccttcgga tttgctggta
     2161 tctatgacaa ggatgaagcc gagcgcattg tcgaaaatac tgcatacact gcagaacgtc
     2221 agccggaacg cgacatcact ccggttaacg atgaaaccat gcaggagatt aacactctgc
     2281 tgatcgccct ggataaaaca tgggatgacg acttattgcc gctctgttcc cagatatttc
     2341 gccgcgacat tcgtgcatcg tcagaactga cacaggccga agcagtaaaa gctcttggat
     2401 tcctgaaaca gaaagccgca gagcagaagg tggcagcatg acaccggaca ttatcctgca
     2461 gcgtaccggg atcgatgtga gagctgtcga acagggggat gatgcgtggc acaaattacg
     2521 gctcggcgtc atcaccgctt cagaagttca caacgtgata gcaaaacccc gctccggaaa
     2581 gaagtggcct gacatgaaaa tgtcctactt ccacaccctg cttgctgagg tttgcaccgg
     2641 tgtggctccg gaagttaacg ctaaagcact ggcctgggga aaacagtacg agaacgacgc
     2701 cagaaccctg tttgaattca cttccggcgt gaatgttact gaatccccga tcatctatcg
     2761 cgacgaaagt atgcgtaccg cctgctctcc cgatggttta tgcagtgacg gcaacggcct
     2821 tgaactgaaa tgcccgttta cctcccggga tttcatgaag ttccggctcg gtggtttcga
     2881 ggccataaag tcagcttaca tggcccaggt gcagtacagc atgtgggtga cgcgaaaaaa
     2941 tgcctggtac tttgccaact atgacccgcg tatgaagcgt gaaggcctgc attatgtcgt
     3001 gattgagcgg gatgaaaagt acatggcgag ttttgacgag atcgtgccgg agttcatcga
     3061 aaaaatggac gaggcactgg ctgaaattgg ttttgtattt ggggagcaat ggcgatgacg
     3121 catcctcacg ataatatccg ggtaggcgca atcactttcg tctactccgt tacaaagcga
     3181 ggctgggtat ttcccggcct ttctgttatc cgaaatccac tgaaagcaca gcggctggct
     3241 gaggagataa ataataaacg aggggctgta tgcacaaagc atcttctgtt gagttaagaa
     3301 cgagtatcga gatggcacat agccttgctc aaattggaat caggtttgtg ccaataccag
     3361 tagaaacaga cgaagaatcc atgatcaccg ctaacctgtc ttttaacctg cttttaaacc
     3421 aatatttata aaccttgttt ttaaccaggg ctgcgccctg tgcgcgtgac cgcgcacgcc
     3481 gaaggggggt gccccccctt ctcgaaccct cccggcccgc taacgcgggc ctcccatccc
     3541 cccaggggct gcgcccctcg gccgcgaacg gcctcacccc aaaaatggca ggggatctcg
     3601 agggatcctt tttgtccggt gttgggttga aggtgaagcc ggtcggggcc gcagcggggg
     3661 ccggcttttc agccttgccc ccctgcttcg gccgccgtgg ctccggcgtc ttgggtgccg
     3721 gcgcgggttc cgcagccttg gcctgcggtg cgggcacatc ggcgggcttg gccttgatgt
     3781 gccgcctggc gtgcgagcgg aacgtctcgt aggagaactt gaccttcccc gtttcccgca
     3841 tgtgctccca aatggtgacg agcgcatagc cggacgctaa cgccgcctcg acatccgccc
     3901 tcaccgccag gaacgcaacc gcagcctcat cacgccggcg cttcttggcc gcgcgggatt
     3961 caacccactc ggccagctcg tcggtgtagc tctttggcat cgtctctcgc ctgtcccctc
     4021 agttcagtaa tttcctgcat ttgcctgttt ccagtcggta gatattccac aaaacagcag
     4081 ggaagcagcg cttttccgct gcataaccct gcttcggggt cattatagcg attttttcgg
     4141 tatatccatc ctttttcgca cgatatacag gattttgcca aagggttcgt gtagactttc
     4201 cttggtgtac ccaacggcgt cagccgggca ggataggtga agtaggccca cccgcgagcg
     4261 ggtgttcctt cttcactgtc ccttattcgc acctggcggt gctcaacggg aatcctgctc
     4321 tgcgaggctg gccggctacc gccggcgtaa cagatgaggg caagcggatg gctgatgaaa
     4381 ccaagccaac caggaagggc agcccaccta tcaaggtgta ctgccttcca gacgaacgaa
     4441 gagcgattga ggaaaaggcg gcggcggccg gcatgagcct gtcggcctac ctgctggccg
     4501 tcggccaggg ctacaaaatc acgggcgtcg tggactatga gcacgtccgc gagctggccc
     4561 gcatcaatgg cgacctgggc cgcctgggcg gcctgctgaa actctggctc accgacgacc
     4621 cgcgcacggc gcggttcggt gatgccacga tcctcgccct gctggcgaag atcgaagaga
     4681 agcaggacga gcttggcaag gtcatgatgg gcgtggtccg cccgagggca gagccatgac
     4741 ttttttagcc gctaaaacgg ccggggggtg cgcgtgattg ccaagcacgt ccccatgcgc
     4801 tccatcaaga agagcgactt cgcggagctg gtgaagtaca tcaccgacga gcaaggcaag
     4861 accgagcgcc tgggtcacgt gcgcgtcacg aactgcgagg caatcaccct gcccgctgtc
     4921 atggccgagg tgatggcgac ccagcacggc aacacccgtt ccgaggccga caagacctat
     4981 cacctgctgg ttagcttccg cgcgggagag aagcccgacg cggagacgtt gcgcgcgatt
     5041 gaggaccgca tctgcgctgg gcttggcttc gccgagcatc agcgcgtcag tgccgtgcat
     5101 cacgacaccg acaacctgca catccatatc gccatcaaca agattcaccc gacccgaaac
     5161 accatccatg agccgtatcg ggcctaccgc gccctcgctg acctctgcgc gacgctcgaa
     5221 cgggactacg ggcttgagcg tgacaatcac gaaacgcggc agcgcgtttc cgagaaccgc
     5281 gcgaacgaca tggagcggca cgcgggcgtg gaaagcctgg tcggctgctc gagggcttcg
     5341 ccctgtcgct cgactgcggc gagcactact ggctgtaaaa ggacagacca catcattatc
     5401 gtgactgact gctgcgtgta gacttccgtt gaactgatgg acttatgtcc catcaggctt
     5461 tgcagaactt tcagcggtat accggcatac agcatgtgca tcgcatagga atggcggaac
     5521 gtatgtggtg tgaccggaac agagaacgtc acaccgtcag cagcagcggc ggcaaccgcc
     5581 tccccaatcc aggtcctgac cgttctgtcc gtcacttccc agatccgcgc tttctctgtc
     5641 cttcctgtgc gacggttacg ccgctccatg agcttatcgc gaataaatac ctgtgacgga
     5701 agatcacttc gcagaataaa taaatcctgg tgtccctgtt gataccggga agccctgggc
     5761 caacttttgg cgaaaatgag acgttgatcg gcacgtaaga ggttccaact ttcaccataa
     5821 tgaaataaga tcactaccgg gcgtattttt tgagttatcg agattttcag gagctaagga
     5881 agctaaaatg gagaaaaaaa tcactggata taccaccgtt gatatatccc aatggcatcg
     5941 taaagaacat tttgaggcat ttcagtcagt tgctcaatgt acctataacc agaccgttca
     6001 gctggatatt acggcctttt taaagaccgt aaagaaaaat aagcacaagt tttatccggc
     6061 ctttattcac attcttgccc gcctgatgaa tgctcatccg gaattccgta tggcaatgaa
     6121 agacggtgag ctggtgatat gggatagtgt tcacccttgt tacaccgttt tccatgagca
     6181 aactgaaacg ttttcatcgc tctggagtga ataccacgac gattcccggc agtttctaca
     6241 catatattcg caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt
     6301 tattgagaat atgtttttcg tctcagccaa tccctgggtg agtttcacca gttttgattt
     6361 aaacgtggcc aatatggaca acttcttcgc ccccgttttc accatgggca aatattatac
     6421 gcaaggcgac aaggtgctga tgccgctggc gattcaggtt catcatgccg tttgtgatgg
     6481 cttccatgtc ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg
     6541 ggcgtaattt ttttaaggca gttattggtg cccttaaacg cctggttgct acgcctgaat
     6601 aagtgataat aagcggatga atggcagaaa ttcgatgata agctgtcaaa catgagaatt
     6661 ggtcgacggc ccgggcggcc gcaaggggtt cgcgttggcc gattcattag atcatgggta
     6721 tggacagttt tccctttgat atgtaacggt gaacagttgt tctacttttg tttgttagtc
     6781 ttgatgcttc actgatagat acaagagcca taagaacctc agatccttcc gtatttagcc
     6841 agtatgttct ctagtgtggt tcgttgtttt tgcgtgagcc atgagaacga accattgaga
     6901 tcatacttac tttgcatgtc actcaaaaat tttgcctcaa aactggtgag ctgaattttt
     6961 gcagttaaag catcgtgtag tgtttttctt agtccgttat gtaggtagga atctgatgta
     7021 atggttgttg gtattttgtc accattcatt tttatctggt tgttctcaag ttcggttacg
     7081 agatccattt gtctatctag ttcaacttgg aaaatcaacg tatcagtcgg gcggcctcgc
     7141 ttatcaacca ccaatttcat attgctgtaa gtgtttaaat ctttacttat tggtttcaaa
     7201 acccattggt taagcctttt aaactcatgg tagttatttt caagcattaa catgaactta
     7261 aattcatcaa ggctaatctc tatatttgcc ttgtgagttt tcttttgtgt tagttctttt
     7321 aataaccact cataaatcct catagagtat ttgttttcaa aagacttaac atgttccaga
     7381 ttatatttta tgaatttttt taactggaaa agataaggca atatctcttc actaaaaact
     7441 aattctaatt tttcgcttga gaacttggca tagtttgtcc actggaaaat ctcaaagcct
     7501 ttaaccaaag gattcctgat ttccacagtt ctcgtcatca gctctctggt tgctttagct
     7561 aatacaccat aagcattttc cctactgatg ttcatcatct gaacgtattg gttataagtg
     7621 aacgataccg tccgttcttt ccttgtaggg ttttcaatcg tggggttgag tagtgccaca
     7681 cagcataaaa ttagcttggt ttcatgctcc gttaagtcat agcgactaat cgctagttca
     7741 tttgctttga aaacaactaa ttcagacata catctcaatt ggtctaggtg attttaatca
     7801 ctataccaat tgagatgggc tagtcaatga taattactag tccttttcct ttgagttgtg
     7861 ggtatctgta aattctgcta gacctttgct ggaaaacttg taaattctgc tagaccctct
     7921 gtaaattccg ctagaccttt gtgtgttttt tttgtttata ttcaagtggt tataatttat
     7981 agaataaaga aagaataaaa aaagataaaa agaatagatc ccagccctgt gtataactca
     8041 ctactttagt cagttccgca gtattacaaa aggatgtcgc aaacgctgtt tgctcctcta
     8101 caaaacagac cttaaaaccc taaaggctta agtagcaccc tcgcaagctc ggttgcggcc
     8161 gcaatcgggc aaatcgctga atattccttt tgtctccgac catcaggcac ctgagtcgct
     8221 gtctttttcg tgacattcag ttcgctgcgc tcacggctct ggcagtgaat gggggtaaat
     8281 ggcactacag gcgcctttta tggattcatg caaggaaact acccataata caagaaaagc
     8341 ccgtcacggg cttctcaggg cgttttatgg cgggtctgct atgtggtgct atctgacttt
     8401 ttgctgttca gcagttcctg ccctctgatt ttccagtctg accacttcgg attatcccgt
     8461 gacaggtcat tcagactggc taatgcaccc agtaaggcag cggtatcatc aacggggtct
     8521 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     8581 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     8641 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     8701 tgtctatttc gttcatccat agtgcctgac tccccgtcgt gtagataact acgatacggg
     8761 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     8821 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     8881 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     8941 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     9001 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     9061 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     9121 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     9181 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     9241 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     9301 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     9361 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     9421 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     9481 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     9541 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     9601 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gcatcgattt
     9661 a
//
