LOCUS       sequence-391497-       11332 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11332)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 11332)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1429
FEATURES             Location/Qualifiers
     source          1..11332
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    7189..7777
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7242..7262)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7660..7671)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7849..8082
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8160..8281
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8197..8216)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8251..8270
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8321..8456
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8476..8495)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8477..8495)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8505..8522)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8505..8521)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8514..8536)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8663..9118
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8750..8769)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8960..8981
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9144..9248
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9249..9317,9318..10109)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9467..9486)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10280..10868
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10769..10788
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11022..11039
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11156..11177
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11192..11197,11198..11215,11216..11222)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11230..11246
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11235..11257
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11254..11270
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11254..11270
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11289..11309
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11291..11309
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggag accaaaggct accacagtct
     4141 ccctgaaggt ctagatatgg aaagacggtg gggtcaagtt tctcaggctg tggagcgttc
     4201 ttccctggga cctacagaga ggaccgatga gaataactac atggagattg tcaacgtaag
     4261 ctgtgtttcc ggtgctattc caaacaacag tactcaagga agcagcaaag aaaaacaaga
     4321 actactccct tgccttcagc aagacaataa tcggcctggg attttaacat ctgatattaa
     4381 aactgagctg gaatctaagg aactttcagc aactgtagct gagtccatgg gtttatatat
     4441 ggattctgta agagatgctg actattccta tgagcagcag aaccaacaag gaagcatgag
     4501 tccagctaag atttatcaga atgttgaaca gctggtgaaa ttttacaaag gaaatggcca
     4561 tcgtccttcc actctaagtt gtgtgaacac gcccttgaga tcatttatgt ctgactctgg
     4621 gagctccgtg aatggtggcg tcatgcgcgc cgttgttaaa agccctatca tgtgtcatga
     4681 gaaaagcccg tctgtttgca gccctctgaa catgacatct tcggtttgca gccctgctgg
     4741 aatcaactct gtgtcctcca ccacagccag ctttggcagt tttccagtgc acagcccaat
     4801 cacccaggga actcctctga catgctcccc taatgttgaa aatcgaggct ccaggtcgca
     4861 cagccctgca catgctagca atgtgggctc tcctctctca agtccgttaa gtagcatgaa
     4921 atcctcaatt tccagccctc caagtcactg cagtgtaaaa tctccagtct ccagtcccaa
     4981 taatgtcact ctgagatcct ctgtgtctag ccctgcaaat attaacaact caaggtgctc
     5041 tgtttccagc ccttcgaaca ctaataacag atccacgctt tccagtccgg cagccagtac
     5101 tgtgggatct atctgtagcc ctgtaaacaa tgccttcagc tacactgctt ctggcacctc
     5161 tgctggatcc agtacattgc gggatgtggt tcccagtcca gacacgcagg agaaaggtgc
     5221 tcaagaggtc ccttttccta agactgagga agtagagagt gccatctcaa atggtgtgac
     5281 tggccagctt aatattgtcc agtacataaa accagaacca gatggagctt ttagcagctc
     5341 atgtctagga ggaaatagca aaataaattc ggattcttca ttctcagtac caataaagca
     5401 agaatcaacc aagcattcat gttcaggcac ctcttttaaa gggaatccaa cagtaaaccc
     5461 gtttccattt atggatggct cgtatttttc ctttatggat gataaagact attattccct
     5521 atcaggaatt ttaggaccac ctgtgcccgg ctttgatggt aactgtgaag gcagcggatt
     5581 cccagtgggt attaaacaag aaccagatga tgggagctat tacccagagg ccagcatccc
     5641 ttcctctgct attgttgggg tgaattcagg tggacagtcc ttccactaca ggattggtgc
     5701 tcaaggtaca atatctttat cacgatcggc tagagaccaa tctttccaac acctgagttc
     5761 ctttcctcct gtcaatactt tagtggagtc atggaaatca cacggcgacc tgtcgtctag
     5821 aagaagtgat gggtatccgg tcttagaata cattccagaa aatgtatcaa gctctacttt
     5881 acgaagtgtt tctactggat cttcaagacc ttcaaaaata tgtttggtgt gtggggatga
     5941 ggcttcagga tgccattatg gggtagtcac ctgtggcagc tgcaaagttt tcttcaaaag
     6001 agcagtggaa ggtaaatgtt catggcaaca caactattta tgtgctggaa gaaatgattg
     6061 catcattgat aagattcgac gaaagaattg tcctgcttgc agacttcaga aatgtcttca
     6121 agctggaatg aatttaggag cacgaaagtc aaagaagttg ggaaagttaa aagggattca
     6181 cgaggagcag ccacagcagc agcagccccc acccccaccc ccacccccgc aaagcccaga
     6241 ggaagggaca acgtacatcg ctcctgcaaa agaaccctcg gtcaacacag cactggttcc
     6301 tcagctctcc acaatctcac gagcgctcac accttccccc gttatggtcc ttgaaaacat
     6361 tgaacctgaa attgtatatg caggctatga cagctcaaaa ccagatacag ccgaaaatct
     6421 gctctccacg ctcaaccgct tagcaggcaa acagatgatc caagtcgtga agtgggcaaa
     6481 ggtacttcca ggatttaaaa acttgcctct tgaggaccaa attaccctaa tccagtattc
     6541 ttggatgtgt ctatcatcat ttgccttgag ctggagatcg tacaaacata cgaacagcca
     6601 atttctctat tttgcaccag acctagtctt taatgaagag aagatgcatc agtctgccat
     6661 gtatgaacta tgccagggga tgcaccaaat cagccttcag ttcgttcgac tgcagctcac
     6721 ctttgaagaa tacaccatca tgaaagtttt gctgctacta agcacaattc caaaggatgg
     6781 cctcaaaagc caggctgcat ttgaagaaat gaggacaaat tacatcaaag aactgaggaa
     6841 gatggtaact aagtgtccca acaattctgg gcagagctgg cagaggttct accaactgac
     6901 caagctgctg gactccatgc atgacctggt gagcgacctg ctggaattct gcttctacac
     6961 cttccgagag tcccatgcgc tgaaggtaga gttccccgca atgctggtgg agatcatcag
     7021 cgaccagctg cccaaggtgg agtcggggaa cgccaagccg ctctacttcc accggaagta
     7081 gtaatgaact agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg
     7141 aaaggacgaa atctcagtct tagtcggcgg agaacgcgtt aagtcgacaa tcaacctctg
     7201 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     7261 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     7321 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     7381 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     7441 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     7501 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     7561 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     7621 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     7681 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     7741 cagacgagtc ggatctccct ttgggccgcc tccccgcgtc gactttaaga ccaatgactt
     7801 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     7861 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     7921 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     7981 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     8041 gatccctcag acccttttag tcagtgtgga aaatctctag cagtacgtat agtagttcat
     8101 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga
     8161 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     8221 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     8281 atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc
     8341 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     8401 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     8461 gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta
     8521 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     8581 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     8641 cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     8701 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     8761 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     8821 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     8881 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     8941 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     9001 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     9061 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag
     9121 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     9181 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     9241 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     9301 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     9361 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     9421 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     9481 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     9541 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     9601 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     9661 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     9721 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     9781 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     9841 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     9901 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     9961 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
    10021 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
    10081 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
    10141 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
    10201 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
    10261 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
    10321 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
    10381 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
    10441 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
    10501 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
    10561 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
    10621 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
    10681 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
    10741 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
    10801 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
    10861 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
    10921 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
    10981 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
    11041 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
    11101 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
    11161 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
    11221 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
    11281 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa gc
//
