LOCUS       sequence-39151-e         747 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Pasque V, Gillich A, Garrett N, Gurdon JB
  TITLE     Histone variant macroH2A confers resistance to nuclear 
            reprogramming.
  JOURNAL   EMBO J. 2011 May 6;30(12):2373-87. doi: 10.1038/emboj.2011.144.
  PUBMED    21552206
REFERENCE   2  (bases 1 to 747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/emboj.2011"; journalName: "EMBO J"; date: "2011-05-6- 6"; 
            volume: "30"; issue: "12"; pages: "2373-87"
COMMENT     Sequence Label: pSUPER retro puro macroH2A1 shRNA
FEATURES             Location/Qualifiers
     source          1..747
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(94..110)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(95..111)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     CDS             complement(129..545)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(145..161)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(177..199)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
ORIGIN
        1 tcttataagt tctgtatgag accacagatc cccgggtgca gacaaatgtg aattcaagag
       61 attcacattt gtctgcaccc tttttaagct tatcgatacc gtcgacctcg agagatcgat
      121 ctaattccgg cgcctagaga aggagtgagg gctggataaa gggaggatcg aggcggggtc
      181 gaacgaggag gttcaagggg gagagacggg gcggatggag gaagaggagg cggaggctta
      241 gggtgtacaa agggcttgac ccagggaggg gggtcaaaag ccaaggcttc ccaggtcacg
      301 atgtagggga cctggtctgg gtgtccatgc gggccaggtg aaaagacctt gatcttaacc
      361 tgggtgatga ggtctcggtt aaaggtgccg tctcgcggcc atccgacgtt aaaggttggc
      421 cattctgcag agcagaaggt aacccaacgt ctcttcttga catctaccga ctggttgtga
      481 gcgatccgct cgacatcttt ccagtgacct aaggtcaaac ttaagggagt ggtaacagtc
      541 tggccctaat tttcagacaa atacagaaac acagtcagac agagacaaca cagaacgatg
      601 ctgcagcgct gcagcagaca agacgcgcgg cttcggttcc aaaccgaaag caaaaattca
      661 gacggaggcg ggaactgttt taggttctcg tctcctacca gaaccacata tcctgacggg
      721 gtcggattcc acatcgactc ccttcct
//
