LOCUS       sequence-391524-       10951 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10951)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0567
FEATURES             Location/Qualifiers
     source          1..10951
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    6808..7396
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6861..6881)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7279..7290)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7468..7701
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7779..7900
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7816..7835)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7870..7889
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7940..8075
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8095..8114)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8096..8114)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8124..8141)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8124..8140)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8133..8155)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8282..8737
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8369..8388)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8579..8600
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8763..8867
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8868..8936,8937..9728)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9086..9105)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9899..10487
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10388..10407
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10641..10658
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10775..10796
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10811..10816,10817..10834,10835..10841)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10849..10865
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10854..10876
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10873..10889
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10873..10889
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10908..10928
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10910..10928
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgaac cagccgggcg gcgcggcggc
     4141 tccgcaggct gacggagcca gtgcagccgg aaggaaaagc actgcgagca gggagcgctt
     4201 gaagcgcagc cagaagagca ccaaggtgga gggcccagag ccagtgccag ccgaggcctc
     4261 gctgagtgcc gagcaaggaa cgatgacgga ggtgaaggtg aagacagagc tgcccgatga
     4321 ctacatccag gaggtgatct ggcagggcga ggccaaggag gagaagaagg cggtcagcaa
     4381 ggatgggacc agcgacgtgc ctgccgagat ctgcgtggtg atcggcggcg tccgcaacca
     4441 gcagaccctt gggtcttacg aatgcggaat ctgtggcaag aagtacaagt attacaactg
     4501 cttccagacc cacgtgcggg cgcaccgaga caccgaagcc acctcagggg agggagcctc
     4561 ccaaagcaac aatttcaggt acacatgtga tatctgcggg aagaagtaca aatactacag
     4621 ctgtttccaa gagcaccgag acctgcacgc agtggatgtg tttagtgtgg aaggggcccc
     4681 tgagaaccgg gcagacccct tcgaccaagg tgtcgtggcc acggacgagg tgaaggagga
     4741 gcccccggag ccattccaga aaatcgggcc aatgaacaat attacatcag acatttttaa
     4801 gaagaaagaa gttaggcagt gccaaaagag agaaactggc aattacacct gtgaattctg
     4861 cggcaaacag tacaagtact acactcccta ccaggagcat gtggccttac acgcccccat
     4921 caaaagcgct ttcagtcgga gagtagaagg caaagcacaa aaccactttg aagagacgaa
     4981 cagcagttcg cagaactcca gcgaacccta cacctgcggc gcctgtggga tccagttcca
     5041 gttctacaac aacctgttgg agcacatgca gtcccatgca gctgacaatg aaaacaacat
     5101 tgcctccaac cagtcccgat cgccacctgc tgttgtagaa gagaagtgga aacctcaggc
     5161 ccagaggaac agtgccaata acaccactac cagtggttta acacccaaca gcatgatccc
     5221 cgaaaaggag cggcagaaca tcgcagagcg gctgttgagg gtcatgtgtg ccgacctggg
     5281 tgcactgagc gtggtcagcg ggaaggagtt cctgaagttg gcccagacct tagtagacag
     5341 tggtgcccgc tatggggcct tctcggtcac tgaaatcctg ggcaacttca acacgctggc
     5401 gctgaagcac ctgccacgca tgtacaacca ggtgaaggtg aaagtgacct gtgccttggg
     5461 cagcaatgcc tgcctaggca tcggtgtcac ctgccactcc cagagtgttg gccctgactc
     5521 ctgctacatc ctcacagcct accaggccga gggcaaccac atcaagagct atgtgcttgg
     5581 tgtgaagggt gcggacattc gcgacagcgg tgaccttgtg caccactggg tgcagaacgt
     5641 gctgtcggag ttcgtgatgt cggagatcag gacagtgtac gtgacggatt gccgggtgag
     5701 cacgtccgcc ttctccaagg ccggcatgtg ccttcgctgc tcagcctgtg ccttgaactc
     5761 ggtggtgcag agcgtgctga gcaagcggac actgcaggcc cgcagcatgc acgaggtcat
     5821 cgagctgctc aacgtgtgcg aggacctggc gggctccacg ggcctggcca aggagacctt
     5881 cgggtcgctg gaggagacgt ctccaccacc ctgctggaac tcggtgacgg actcactgtt
     5941 gctggtgcat gagcgctatg agcagatctg cgagttctac agccgggcca agaagatgaa
     6001 cctcatccag agcctcaaca agcacctgct cagcaacctg gcggccatcc tgacgccggt
     6061 gaagcaggca gtcatcgagc tgagcaacga gagccagccc accctgcagc tggtgctgcc
     6121 cacctacgtc aggctggaga agctgttcac ggccaaggcc aacgacgcag gcactgtcag
     6181 caagctctgc cacctcttcc tggaggcgct caaggagaac ttcaaggtgc acccggccca
     6241 caaggtggcc atgatcctgg acccgcagca gaagctgcgg cctgtgccac cctaccagca
     6301 cgaggagatc atcggcaagg tctgtgagct catcaacgag gtgaaggagt cctgggccga
     6361 ggaggccgac ttcgagcccg ctgccaagaa gccccgctct gctgccgtcg agaaccccgc
     6421 agctcaggaa gatgatcggc taggcaaaaa tgaagtgtac gattacctgc aggagcccct
     6481 cttccaggct acccctgatc tcttccagta ctggtcgtgc gttacccaaa agcacacaaa
     6541 actcgccaag ctcgccttct ggctcctggc ggttccggcc gtgggcgcca gaagcgggtg
     6601 tgtaaatatg tgtgaacaag cgcttctaat caaacggagg cggctgctca gtccagaaga
     6661 tatgaataaa ctcatgtttc tgaaatccaa catgctttag taatgaacta gtccgtaact
     6721 tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaca atttctctat
     6781 gtaatgctcg acacgcgtta agtcgacaat caacctctgg attacaaaat ttgtgaaaga
     6841 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     6901 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     6961 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     7021 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     7081 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     7141 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     7201 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     7261 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     7321 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     7381 tgggccgcct ccccgcgtcg actttaagac caatgactta caaggcagct gtagatctta
     7441 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     7501 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     7561 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     7621 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     7681 cagtgtggaa aatctctagc agtacgtata gtagttcatg tcatcttatt attcagtatt
     7741 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata
     7801 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     7861 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat
     7921 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     7981 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     8041 attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat
     8101 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     8161 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     8221 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     8281 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     8341 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     8401 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     8461 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     8521 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     8581 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     8641 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     8701 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg
     8761 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     8821 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     8881 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     8941 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     9001 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     9061 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     9121 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     9181 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     9241 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     9301 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     9361 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     9421 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     9481 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     9541 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     9601 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     9661 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     9721 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     9781 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     9841 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     9901 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     9961 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
    10021 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
    10081 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
    10141 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
    10201 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
    10261 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
    10321 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
    10381 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
    10441 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
    10501 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
    10561 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
    10621 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
    10681 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
    10741 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
    10801 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
    10861 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
    10921 actaaaggga acaaaagctg gagctgcaag c
//
