LOCUS       sequence-391546-       10666 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10666)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10666)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1871
FEATURES             Location/Qualifiers
     source          1..10666
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    6523..7111
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6576..6596)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6994..7005)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7183..7416
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7494..7615
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7531..7550)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7585..7604
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7655..7790
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7810..7829)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7811..7829)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7839..7856)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7839..7855)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7848..7870)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7997..8452
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8084..8103)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8294..8315
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8478..8582
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8583..8651,8652..9443)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8801..8820)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9614..10202
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10103..10122
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10356..10373
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10490..10511
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10526..10531,10532..10549,10550..10556)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10564..10580
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10569..10591
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10588..10604
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10588..10604
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10623..10643
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10625..10643
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggcg gacggaggag cagcgagtca
     4141 agatgagagt tcagccgcgg cggcagcagc agcagactca agaatgaaca atccgtcaga
     4201 aaccagtaaa ccatctatgg agagtggaga tggcaacaca ggcacacaaa ccaatggtct
     4261 ggactttcag aagcagcctg tgcctgtagg aggagcaatc tcaacagccc aggcgcaggc
     4321 tttccttgga catctccatc aggtccaact cgctggaaca agtttacagg ctgctgctca
     4381 gtctttaaat gtacagtcta aatctaatga agaatcgggg gattcgcagc agccaagcca
     4441 gccttcccag cagccttcag tgcaggcagc cattccccag acccagctta tgctagctgg
     4501 aggacagata actgggctta ctttgacgcc tgcccagcaa cagttactac tccagcaggc
     4561 acaggcacag gcacagctgc tggctgctgc agtgcagcag cactccgcca gccagcagca
     4621 cagtgctgct ggagccacca tctccgcctc tgctgccacg cccatgacgc agatccccct
     4681 gtctcagccc atacagatcg cacaggatct tcaacaactg caacagcttc aacagcagaa
     4741 tctcaacctg caacagtttg tgttggtgca tccaaccacc aatttgcagc cagcgcagtt
     4801 tatcatctca cagacgcccc agggccagca gggtctcctg caagcgcaaa atcttctaac
     4861 gcaactacct cagcaaagcc aagccaacct cctacagtcg cagccaagca tcaccctcac
     4921 ctcccagcca gcaaccccaa cacgcacaat agcagcaacc ccaattcaga cacttccaca
     4981 gagccagtca acaccaaagc gaattgatac tcccagcttg gaggagccca gtgaccttga
     5041 ggagcttgag cagtttgcca agaccttcaa acaaagacga atcaaacttg gattcactca
     5101 gggtgatgtt gggctcgcta tggggaaact atatggaaat gacttcagcc aaactaccat
     5161 ctctcgattt gaagccttga acctcagctt taagaacatg tgcaagttga agccactttt
     5221 agagaagtgg ctaaatgatg cagagaacct ctcatctgat tcgtccctct ccagcccaag
     5281 tgccctgaat tctccaggaa ttgagggctt gagccgtagg aggaagaaac gcaccagcat
     5341 agagaccaac atccgtgtgg ccttagagaa gagtttcttg gagaatcaaa agcctacctc
     5401 ggaagagatc actatgattg ctgatcagct caatatggaa aaagaggtga ttcgtgtttg
     5461 gttctgtaac cgccgccaga aagaaaaaag aatcaaccca ccaagcagtg gtgggaccag
     5521 cagctcacct attaaagcaa ttttccccag cccaacttca ctggtggcga ccacaccaag
     5581 ccttgtgact agcagtgcag caactaccct cacagtcagc cctgtcctcc ctctgaccag
     5641 tgctgctgtg acgaatcttt cagttacagg cacttcagac accacctcca acaacacagc
     5701 aaccgtgatt tccacagcgc ctccagcttc ctcagcagtc acgtccccct ctctgagtcc
     5761 ctccccttct gcctcagcct ccacctccga ggcatccagt gccagtgaga ccagcacaac
     5821 acagaccacc tccactcctt tgtcctcccc tcttgggacc agccaggtga tggtgacagc
     5881 atcaggtttg caaacagcag cagctgctgc ccttcaagga gctgcacagt tgccagcaaa
     5941 tgccagtctt gctgccatgg cagctgctgc aggactaaac ccaagcctga tggcaccctc
     6001 acagtttgcg gctggaggtg ccttactcag tctgaatcca gggaccctga gcggtgctct
     6061 cagcccagct ctaatgagca acagtacact ggcaactatt caagctcttg cttctggtgg
     6121 ctctcttcca ataacatcac ttgatgcaac tgggaacctg gtatttgcca atgcgggagg
     6181 agcccccaac atcgtgactg cccctctgtt cctgaaccct cagaacctct ctctgctcac
     6241 cagcaaccct gttagcttgg tctctgccgc cgcagcatct gcagggaact ctgcacctgt
     6301 agccagcctt cacgccacct ccacctctgc tgagtccatc cagaactctc tcttcacagt
     6361 ggcctctgcc agcggggctg cgtccaccac caccaccgcc tccaaggcac agtagtaatg
     6421 aactagtccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
     6481 cgacgcaggg ctccaaatag acgacgcacg cgttaagtcg acaatcaacc tctggattac
     6541 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     6601 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     6661 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     6721 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     6781 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     6841 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     6901 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     6961 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     7021 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     7081 agtcggatct ccctttgggc cgcctccccg cgtcgacttt aagaccaatg acttacaagg
     7141 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     7201 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     7261 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     7321 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     7381 tcagaccctt ttagtcagtg tggaaaatct ctagcagtac gtatagtagt tcatgtcatc
     7441 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
     7501 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     7561 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     7621 tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     7681 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     7741 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta
     7801 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
     7861 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     7921 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     7981 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     8041 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     8101 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     8161 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     8221 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     8281 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     8341 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     8401 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
     8461 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     8521 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     8581 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     8641 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     8701 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     8761 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     8821 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     8881 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     8941 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     9001 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     9061 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     9121 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     9181 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     9241 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     9301 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     9361 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     9421 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     9481 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     9541 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     9601 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     9661 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     9721 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     9781 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     9841 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     9901 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     9961 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
    10021 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
    10081 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
    10141 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
    10201 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
    10261 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
    10321 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
    10381 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
    10441 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
    10501 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
    10561 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
    10621 gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagc
//
