LOCUS       sequence-391627-         261 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of human transforming growth factor alpha with 
            a peptide tag for covalent bond formation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 261)
  AUTHORS   Keeble AH, Wood DP, Howarth M
  TITLE     Design and Evolution of Enhanced Peptide-Peptide Ligation for 
            Modular Transglutaminase Assembly.
  JOURNAL   Bioconjug Chem. 2023 Jun 8. doi: 10.1021/acs.bioconjchem.3c00122.
  PUBMED    37289810
REFERENCE   2  (bases 1 to 261)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Bioconjug 
            Chem. 2023 Jun 8. doi: 10.1021/acs.bioconjchem.3c00122."
COMMENT     Sequence Label: pET28a-SnoopTag2-TGF-alpha
FEATURES             Location/Qualifiers
     source          1..261
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..39
                     /label=SnoopTag2
     CDS             217..234
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgaaactgg gctatattga attttataaa gtggaaaaag gcagcagcgt cgtctcccat
       61 tttaacgatt gccccgacag ccacacacaa ttctgttttc atgggacctg tcggttcttg
      121 gtacaggagg ataaacctgc ttgcgtgtgc catagcggct acgtcggggc aagatgcgag
      181 cacgcggatc tgttggctgg cagcagcggc agcagccatc atcaccatca ccacagcagc
      241 ggcgagccag aggcttaata a
//
