LOCUS       sequence-391628-        1527 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of a modified Transglutaminase 2 with a peptide
            tag for modular ligation directed by SnoopLigase2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Keeble AH, Wood DP, Howarth M
  TITLE     Design and Evolution of Enhanced Peptide-Peptide Ligation for 
            Modular Transglutaminase Assembly.
  JOURNAL   Bioconjug Chem. 2023 Jun 8. doi: 10.1021/acs.bioconjchem.3c00122.
  PUBMED    37289810
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Bioconjug 
            Chem. 2023 Jun 8. doi: 10.1021/acs.bioconjchem.3c00122."
COMMENT     Sequence Label: pET28a-TG2x-DogTag2
FEATURES             Location/Qualifiers
     source          1..1527
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1405..1473
                     /codon_start=1
                     /product="peptide derived from the RrgA adhesin of 
                     Streptococcus pneumoniae"
                     /label=DogTag
                     /note="forms a covalent bond with the DogCatcher protein 
                     when inserted into an intra-domain protein loop (Keeble et 
                     al., 2022)"
                     /translation="DIPATYEFTDGKHYITNEPLPPK"
     CDS             1492..1521
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
ORIGIN
        1 atggccgaag aactggttct tgagcgctgt gacttggaac tcgagacgaa tgggcgggac
       61 caccatactg ccgacctctg ccgggaaaag ctggtcgtcc gtcgtggtca accattctgg
      121 ttaaccttac attttgaagg gcgtaattac gaggctagtg ttgactcctt aactttcagt
      181 gttgtaactg gtccggcccc atcccaggaa gcaggcacga aggcccgttt cccattgcgt
      241 gacgctgtcg aggaaggtga ttggactgca accgtggtcg atcaacaaga ttgcactctt
      301 agtctgcagt tgaccactcc agcaaatgcc ccgatcggtt tgtaccgttt aagcttggag
      361 gcgtcgaccg gttaccaagg ttcgagcttc gtactcgggc attttatcct cctgtttaac
      421 gcttggtgcc cggccgatgc agtttatttg gatagtgagg aggagcgcca agagtatgtc
      481 ttgacccaac agggtttcat ctatcagggg tccgcgaagt tcattaagaa tattccgtgg
      541 aacttcgggc aattcgagga cggcatcctg gacatctgtc tcattctcct tgacgtcaac
      601 ccaaagtttt taaagaacgc tgggcgggat tgttcccggc ggtccagtcc agtatacgta
      661 gggcgggttg tgtcagggat ggtaaacagc aacgatgatc agggcgttct cttgggccgg
      721 tgggacaaca attatggcga tggggtgtcc ccgatgagtt ggattggtag cgtggacatc
      781 ttgcggcgtt ggaagaacca cggttgtcag cgggtgaagt acggccaatg ctgggtgttc
      841 gccgcagtag cttgcactgt attacggtgt ttgggtatcc caacgcgggt cgttacaaat
      901 tacaactcgg cacatgatca gaactctaac ttactgatcg agtatttccg caacgaattt
      961 ggggagatcc agggcgataa aagcgaaatg atttggaatt tccattgttg ggtggagagc
     1021 tggatgacac gtccggattt acagccgggt tacgaaggct ggcaagcact ggatccaaca
     1081 cctcaggaaa aaagtgaagg gacgtattgt tgcggccctg tgcctgtccg cgcgatcaaa
     1141 gagggcgacc tctccacaaa gtacgatgca ccatttgtct tcgcggaggt gaacgctgac
     1201 gtggttgact ggattcaaca ggatgacggg tctgtacata aatcgatcaa tcgttcgtta
     1261 atcgttggcc ttaagattag tacaaagtcg gttggccgcg atgagcgcga agatatcacc
     1321 cacacgtaca aataccctga gggctcttcg gaagagcgtg aagctttcac ccgggcaaac
     1381 catttgaata aattaggcag ctctgatatt ccggctacat acgaattcac cgatggtaaa
     1441 cattatatca ccaatgaacc gctgccgccg aaaggtagtg gtgaaagtgg tcaccatcac
     1501 catcaccatc accatcacca ttaataa
//
