LOCUS       sequence-391631-        1362 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of SnoopLigase 2 which catalyzes formation of a
            covalent bond between SnoopTag2 and DogTag2 peptides. HaloTag7 
            allows covalent coupling to HaloLink Magne beads..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Keeble AH, Wood DP, Howarth M
  TITLE     Design and Evolution of Enhanced Peptide-Peptide Ligation for 
            Modular Transglutaminase Assembly.
  JOURNAL   Bioconjug Chem. 2023 Jun 8. doi: 10.1021/acs.bioconjchem.3c00122.
  PUBMED    37289810
REFERENCE   2  (bases 1 to 1362)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Bioconjug 
            Chem. 2023 Jun 8. doi: 10.1021/acs.bioconjchem.3c00122."
COMMENT     Sequence Label: pET28a-HaloTag7-SnoopLigase2
FEATURES             Location/Qualifiers
     source          1..1362
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             40..924
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRSIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNSKAVDIGPGLNLLQEDNPDLIG
                     SEIARWLSTLEISG"
ORIGIN
        1 atgggcagca gccatcatca tcatcatcac agcagcggcg aaatcggtac tggctttcca
       61 ttcgaccccc attatgtgga agtcctgggc gagcgcatgc actacgtcga tgttggtccg
      121 cgcgatggca cccctgtgct gttcctgcac ggtaacccga cctcctccta cgtgtggcgc
      181 aacatcatcc cgcatgttgc accgacccat cgcagcattg ctccagacct gatcggtatg
      241 ggcaaatccg acaaaccaga cctgggttat ttcttcgacg accacgtccg cttcatggat
      301 gccttcatcg aagccctggg tctggaagag gtcgtcctgg tcattcacga ctggggctcc
      361 gctctgggtt tccactgggc caagcgcaat ccagagcgcg tcaaaggtat tgcatttatg
      421 gagttcatcc gccctatccc gacctgggac gaatggccag aatttgcccg cgagaccttc
      481 caggccttcc gcaccaccga cgtcggccgc aagctgatca tcgatcagaa cgtttttatc
      541 gagggtacgc tgccgatggg tgtcgtccgc ccgctgactg aagtcgagat ggaccattac
      601 cgcgagccgt tcctgaatcc tgttgaccgc gagccactgt ggcgcttccc aaacgagctg
      661 ccaatcgccg gtgagccagc gaacatcgtc gcgctggtcg aagaatacat ggactggctg
      721 caccagtccc ctgtcccgaa gctgctgttc tggggcaccc caggcgttct gatcccaccg
      781 gccgaagccg ctcgcctggc caaaagcctg cctaacagca aggctgtgga catcggcccg
      841 ggtctgaatc tgctgcaaga agacaacccg gacctgatcg gcagcgagat cgcgcgctgg
      901 ctgtctactc tggagatttc cggtggatcc cccgccaacc tgaaggccct ggaggcccag
      961 aagcagaagg agcagagaca ggccgccgag gagctggcca acgccaagaa gctgaaggag
     1021 cagctggaga agggatccca tatggtgaat aagaacgata aaaagccgct gcgtggtgcc
     1081 gtgtttagcc tgcagaaaca gcatcccgac tatcccgata tctatggcgc gattgatcag
     1141 aatgggacct atcaaaatgt gcgtaccggc gaagatggta aactgacctt taagaatctg
     1201 agcgatggca aatatcgcct gattgaaaat agcgaacccc cgggctataa accggtgcag
     1261 aataagccga ttgtgagctt tcgtattgtg gatggcgaag tgcgtgatgt gaccagcatt
     1321 gtgccgccgg gtgtgccggc tacatatgaa tttacctaat aa
//
