LOCUS       sequence-391632-         402 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of SUMO (Small Ubiquitin-Like Modifier) with 
            peptide tag for covalent bond formation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Keeble AH, Wood DP, Howarth M
  TITLE     Design and Evolution of Enhanced Peptide-Peptide Ligation for 
            Modular Transglutaminase Assembly.
  JOURNAL   Bioconjug Chem. 2023 Jun 8. doi: 10.1021/acs.bioconjchem.3c00122.
  PUBMED    37289810
REFERENCE   2  (bases 1 to 402)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Bioconjug 
            Chem. 2023 Jun 8. doi: 10.1021/acs.bioconjchem.3c00122."
COMMENT     Sequence Label: pET28a SUMO-DogTag2
FEATURES             Location/Qualifiers
     source          1..402
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..33
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgggtagct ggagccatca ccatcatcat cacgggagcg atagcgaagt gaaccaggaa
       61 gcgaaaccgg aagttaaacc ggaagtgaaa ccggaaaccc atattaatct gaaagttagc
      121 gacggcagca gcgaaatctt ttttaaaatt aaaaaaacca ccccgctgcg tcgcctgatg
      181 gaagcctttg cgaaacgtca gggtaaagaa atggatagcc tgcgctttct gtatgacggc
      241 atccgtattc aggccgatca gaccccggaa gacctggata tggaagacaa cgatattatt
      301 gaagcgcatc gcgaacagat cggtggtggc gatattccgg ctacatatga atttaccgat
      361 ggtaaacatt atatcaccaa tgaaccgctg ccgccgaaat aa
//
