LOCUS       sequence-391633-         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of SARS-CoV-2 Receptor Binding Domain with 
            peptide tags for covalent bond formation .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Keeble AH, Wood DP, Howarth M
  TITLE     Design and Evolution of Enhanced Peptide-Peptide Ligation for 
            Modular Transglutaminase Assembly.
  JOURNAL   Bioconjug Chem. 2023 Jun 8. doi: 10.1021/acs.bioconjchem.3c00122.
  PUBMED    37289810
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Bioconjug 
            Chem. 2023 Jun 8. doi: 10.1021/acs.bioconjchem.3c00122."
COMMENT     Sequence Label: pcDNA3.1 RBD-DogTag2-SpyTag
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(398..415)
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
     CDS             691..759
                     /codon_start=1
                     /product="peptide derived from the RrgA adhesin of 
                     Streptococcus pneumoniae"
                     /label=DogTag
                     /note="forms a covalent bond with the DogCatcher protein 
                     when inserted into an intra-domain protein loop (Keeble et 
                     al., 2022)"
                     /translation="DIPATYEFTDGKHYITNEPLPPK"
     CDS             778..816
                     /codon_start=1
                     /product="peptide derived from the CnaB2 adhesin domain of 
                     Streptococcus pyogenes FbaB"
                     /label=SpyTag
                     /note="forms a covalent isopeptide bond with the SpyCatcher
                     protein (Zakeri et al., 2012)"
                     /translation="AHIVMVDAYKPTK"
ORIGIN
        1 atgaacaccc agatcctggt gttcgccctg atcgccatca ttcccaccaa cgccgacaag
       61 atcggatctg gatccaacat caccaatctg tgccccttcg gcgaggtgtt caatgccaca
      121 agatttgcca gcgtgtacgc ctggaaccgg aagagaatca gcaactgcgt ggccgactac
      181 agcgtgctgt acaatagcgc cagcttcagc accttcaagt gctacggcgt gtcccctacc
      241 aagctgaacg acctgtgctt caccaatgtg tacgccgaca gcttcgtgat cagaggcgac
      301 gaagttcggc agatcgctcc tggacagaca ggcaagatcg ccgattacaa ctacaagctg
      361 cccgacgact tcaccggctg cgtgatcgcc tggaatagca acaacctgga cagcaaagtc
      421 ggcggcaact acaactacct gtaccggctg ttccggaagt ccaacctgaa gcctttcgag
      481 cgggacatca gcaccgagat ctatcaggcc ggcagcaccc cttgtaatgg cgtggaaggc
      541 ttcaactgct acttcccact gcagtcctac ggcttccagc ctacaaatgg cgtcggctac
      601 cagccttaca gagtggtggt gctgtccttc gaactgctgc atgcccctgc taccgtgtgc
      661 ggccctaaaa aaaccggagg aggtggctct gatattccgg ctacatacga attcaccgat
      721 ggtaaacatt atatcaccaa tgaaccgctg ccgccgaaag gtagcggcgg atctggagcc
      781 cacatcgtga tggttgatgc ctacaagccc accaaatagt ga
//
