LOCUS       sequence-391635-        1062 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transferrin receptor (TfR)-based construct to display superfolder 
            green fluorescent protein (sfGFP) linked to SnoopTag2 on the surface
            of mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Keeble AH, Wood DP, Howarth M
  TITLE     Design and Evolution of Enhanced Peptide-Peptide Ligation for 
            Modular Transglutaminase Assembly.
  JOURNAL   Bioconjug Chem. 2023 Jun 8. doi: 10.1021/acs.bioconjchem.3c00122.
  PUBMED    37289810
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Bioconjug 
            Chem. 2023 Jun 8. doi: 10.1021/acs.bioconjchem.3c00122."
COMMENT     Sequence Label: pENTR4 TfR-sfGFP-SnoopTag2
FEATURES             Location/Qualifiers
     source          1..1062
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             283..996
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             1009..1044
                     /label=SnoopTag2
ORIGIN
        1 atgatggatc aagctagatc agcattctct aacttgtttg gtggagaacc attgtcatgt
       61 acccgggcca gcctggctcg gcaagtagat ggcgataaca gtcatgtgga gatgaaactt
      121 gctgtagatg aagaagaaaa tgctgacaat aacacaaagg ccaatgtcac aaaaccaaaa
      181 aggtgtagtg gaagtatctg ctatgggact attgctgtga tcgtcttttt cttgattgga
      241 tttatgattg gctacttggg ctatggaagc ggaggatccg ggatgcgtaa aggcgaagag
      301 ctgttcactg gtgtcgtccc tattctggtg gaactggatg gtgatgtcaa cggtcataag
      361 ttttccgtgc gtggcgaggg tgaaggtgac gcaactaatg gtaaactgac gctgaagttc
      421 atctgtacta ctggtaaact gccggtacct tggccgactc tggtaacgac gctgacttat
      481 ggtgttcagt gctttgctcg ttatccggac catatgaagc agcatgactt cttcaagtcc
      541 gccatgccgg aaggctatgt gcaggaacgc acgatttcct ttaaggatga cggcacgtac
      601 aaaacgcgtg cggaagtgaa atttgaaggc gataccctgg taaaccgcat tgagctgaaa
      661 ggcattgact ttaaagaaga cggcaatatc ctgggccata agctggaata caattttaac
      721 agccacaatg tttacatcac cgccgataaa caaaaaaatg gcattaaagc gaattttaaa
      781 attcgccaca acgtggagga tggcagcgtg cagctggctg atcactacca gcaaaacact
      841 ccaatcggtg atggtcctgt tctgctgcca gacaatcact atctgagcac gcaaagcgtt
      901 ctgtctaaag atccgaacga gaaacgcgat catatggttc tgctggagtt cgtaaccgca
      961 gcgggcatca cgcatggtat ggatgaacta tacaaaagca gcggcgggaa actgggctat
     1021 attgaatttt ataaagtgga aaaaggatct agcgtatctt aa
//
