LOCUS       sequence-391636-         369 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of SnoopLigase2 which directs formation of an 
            isopeptide bond between SnoopTag2 and DogTag2 peptides.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Keeble AH, Wood DP, Howarth M
  TITLE     Design and Evolution of Enhanced Peptide-Peptide Ligation for 
            Modular Transglutaminase Assembly.
  JOURNAL   Bioconjug Chem. 2023 Jun 8. doi: 10.1021/acs.bioconjchem.3c00122.
  PUBMED    37289810
REFERENCE   2  (bases 1 to 369)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Bioconjug 
            Chem. 2023 Jun 8. doi: 10.1021/acs.bioconjchem.3c00122."
COMMENT     Sequence Label: pET28a SnoopLigase2
FEATURES             Location/Qualifiers
     source          1..369
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..33
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgggcagct ggagccatca tcatcatcat cacagctctg gtggtagtgg tgtgaataag
       61 aacgataaaa agccgctgcg tggtgccgtg tttagcctgc agaaacagca tcccgactat
      121 cccgatatct atggcgcgat tgatcagaat gggacctatc aaaatgtgcg taccggcgaa
      181 gatggtaaac tgacctttaa gaatctgagc gatggcaaat atcgcctgat tgaaaatagc
      241 gaacccccgg gctataaacc ggtgcagaat aagccgattg tgagctttcg tattgtggat
      301 ggcgaagtgc gtgatgtgac cagcattgtg ccgccgggtg tgccggctac atatgaattt
      361 acctaataa
//
