LOCUS       sequence-391640-        6941 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral expression of Mef2c.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6941)
  AUTHORS   Wang H, Keepers B, Qian Y, Xie Y, Colon M, Liu J, Qian L
  TITLE     Cross-lineage potential of Ascl1 uncovered by comparing diverse 
            reprogramming regulatomes.
  JOURNAL   Cell Stem Cell. 2022 Oct 6;29(10):1491-1504.e9. doi: 
            10.1016/j.stem.2022.09.006.
  PUBMED    36206732
REFERENCE   2  (bases 1 to 6941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2022.09"; journalName: "Cell Stem Cell"; date: 
            "2022-10-6- 6"; volume: "29"; issue: "10"; pages: "1491-1504.e9"
COMMENT     Sequence Label: pMX-Mef2c-puro
FEATURES             Location/Qualifiers
     source          1..6941
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             26..619
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    682..1039
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1098..1514
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1444..1466
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1482..1498
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1524..1898
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1836..1852
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     primer_bind     complement(3280..3300)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3285..3614
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3465..3600
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3624..4223
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3624..3643)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4120..4140
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             4450..4919
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(5049..5066)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5220..5808)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5300..5319)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5972..6763,6764..6832))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6595..6614
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6833..6937)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttccccgaa aagtgccacc tgcataatga aagaccccac ctgtaggttt ggcaagctag
       61 cttaagtaac gccattttgc aaggcatgga aaaatacata actgagaata gaaaagttca
      121 gatcaaggtc aggaacagat ggaacagctg aatatgggcc aaacaggata tctgtggtaa
      181 gcagttcctg ccccggctca gggccaagaa cagatggaac agctgaatat gggccaaaca
      241 ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggtccccaga
      301 tgcggtccag ccctcagcag tttctagaga accatcagat gtttccaggg tgccccaagg
      361 acctgaaatg accctgtgcc ttatttgaac taaccaatca gttcgcttct cgcttctgtt
      421 cgcgcgcttc tgctccccga gctcaataaa agagcccaca acccctcact cggcgcgcca
      481 gtcctccgat tgactgagtc gcccgggtac ccgtgtatcc aataaaccct cttgcagttg
      541 catccgactt gtggtctcgc tgttccttgg gagggtctcc tctgagtgat tgactacccg
      601 tcagcggggg tctttcattt gggggctcgt ccgggatcgg gagacccctg cccagggacc
      661 accgacccac caccgggagg taagctggcc agcaacttat ctgtgtctgt ccgattgtct
      721 agtgtctatg actgatttta tgcgcctgcg tcggtactag ttagctaact agctctgtat
      781 ctggcggacc cgtggtggaa ctgacgagtt cggaacaccc ggccgcaacc ctgggagacg
      841 tcccagggac ttcgggggcc gtttttgtgg cccgacctga gtccaaaaat cccgatcgtt
      901 ttggactctt tggtgcaccc ccctaatagg agggatatgt ggttctggta ggagacgaga
      961 acctaaaaca gttcccgcct ccgtctgaat ttttgctttc ggtttgggac cgaagccgcg
     1021 ccgcgcgtct tgtctgctgc agcatcgttc tgtgttgtct ctgtctgact gtgtttctgt
     1081 atttgtctga aaattagggc cagactgtta ccactccctt aagtttgacc ttaggtcact
     1141 ggaaagatgt cgagcggatc gctcacaacc agtcggtaga tgtcaagaag agacgttggg
     1201 ttaccttctg ctctgcagaa tggccaacct ttaacgtcgg atggccgcga gacggcacct
     1261 ttaaccgaga cctcatcacc caggttaaga tcaaggtctt ttcacctggc ccgcatggac
     1321 acccagacca ggtcccctac atcgtgacct gggaagcctt ggcttttgac ccccctccct
     1381 gggtcaagcc ctttgtacac cctaagcctc cgcctcctct tcctccatcc gccccgtctc
     1441 tcccccttga acctcctcgt tcgaccccgc ctcgatcctc cctttatcca gccctcactc
     1501 cttctctagg cgcccccata tggccatatg agatcttata tggggcaccc ccgccccttg
     1561 taaacttccc tgaccctgac atgacaagag ttactaacag cccctctctc caagctcact
     1621 tacaggctct ctacttagtc cagcacgaag tctggagacc tctggcggca gcctaccaag
     1681 aacaactgga ccgaccggtg gtacctcacc cttaccgagt cggcgacaca gtgtgggtcc
     1741 gccgacacca gactaagaac ctagaacctc gctggaaagg accttacaca gtcctgctga
     1801 ccacccccac cgccctcaaa gtagacggca tcgcagcttg gatacacgcc gcccacgtga
     1861 aggctgccga ccccgggggt ggaccatcct ctagactgcc ggatctagct agttaattaa
     1921 ggatccacta tggggagaaa aaagattcag attacgagga taatggatga gcgtaacaga
     1981 caggtgactt ttacgaagag gaaatttgga ttgatgaaga aggcttatga gctgagcgtg
     2041 ctgtgcgact gtgagattgc actgatcatc ttcaacagca ccaacaagct gttccagtac
     2101 gccagcactg acatggataa ggtgttgctc aagtacaccg agtacaacga gccgcacgag
     2161 agccggacaa actcagacat tgtggaggca ttgaacaaga aagaaaacaa aggctctgaa
     2221 agccccgatc ctgactcctc ttatgcactc accccacgca ctgaagaaaa atacaaaaaa
     2281 attaatgaag aatttgataa tatgatcaag agtcataaaa ttcctgctgt tccacctccc
     2341 agctttgaga tgccagttac catcccagtg tccagccata acagtttggt gtacagcaat
     2401 cctgtcagca cactgggaaa ccccaatctt ctgccactgg cccacccgtc tctgcagagg
     2461 aatagtatgt ctcctggtgt aacacataga cctccaagtg caggtaacac aggcggtctg
     2521 atgggcggag atctgacatc cggtgcaggc accagcgcag ggaatggata cggcaacccc
     2581 cggaactcac caggcctgct ggtctcacct ggtaacctga acaagaatat acaagccaaa
     2641 tctcctcccc ctatgaatct aggaatgaat aatcgtaagc cagatctccg cgttcttatc
     2701 ccacctggca gcaagaacac gatgccatca gtgaatcaaa ggataaataa ctcccagtcg
     2761 gctcagtcat tggctacccc ggtggtttcc gtagcaactc ctactttacc aggacaagga
     2821 atgggaggat atccatcagc catttcaaca acatatggta ctgagtactc tctgagtagc
     2881 gcagatctgt catctctgtc tggcttcaac actgccagtg cgctccacct cggctctgta
     2941 actggctggc agcagcagca cctacataac atgccgccat ctgccctcag tcagttggga
     3001 gaccgtacca ccaccccttc gagataccca caacacacca cgcgccacga ggcggggagg
     3061 tctcctgttg acagcttgag cagctgtagc agttcctacg atgggagcga ccgagaggat
     3121 caccggaacg aattccactc ccccattgga ctcaccagac cttcgccgga cgaaagggaa
     3181 agtccttcag tcaagcgcat gcgactctct gaaggatggg caacatgagc ggccgccagc
     3241 acagtggtcg agcggccgcc agcacagtgg tcgacctgtg gaatgtgtgt cagttagggt
     3301 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     3361 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     3421 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     3481 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     3541 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     3601 taggcttttg caaaaagctt accatgaccg agtacaagcc cacggtgcgc ctcgccaccc
     3661 gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca
     3721 cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct
     3781 tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg
     3841 tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc
     3901 cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc
     3961 tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg
     4021 accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc
     4081 gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc
     4141 ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca
     4201 tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc gcagcgcccg accgaaagga
     4261 gcgcacgacc ccatgcatcg tcgacgataa aataaaagat tttatttagt ctccagaaaa
     4321 aggggggaat gaaagacccc acctgtaggt ttggcaagct agcttaagta acgccatttt
     4381 gcaaggcatg gaaaaataca taactgagaa tagagaagtt cagatcaagg tcaggaacag
     4441 atggaacagc tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct
     4501 cagggccaag aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca
     4561 gttcctgccc cggctcaggg ccaagaacag atggtcccca gatgcggtcc agccctcagc
     4621 agtttctaga gaaccatcag atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg
     4681 ccttatttga actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc
     4741 gagctcaata aaagagccca caacccctca ctcggggcgc cagtcctccg attgactgag
     4801 tcgcccgggt acccgtgtat ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc
     4861 gctgttcctt gggagggtct cctctgagtg attgactacc cgtcagcggg ggtctttcac
     4921 atgcagcatg tatcaaaatt aatttggttt tttttcttaa gtatttacat taaatggcca
     4981 tagttgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     5041 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     5101 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     5161 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     5221 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     5281 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     5341 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     5401 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     5461 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     5521 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     5581 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     5641 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     5701 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     5761 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     5821 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     5881 atgagattat caaaaaggat cttcacctag atccttttgc ggccggccgc aaatcaatct
     5941 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     6001 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     6061 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     6121 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     6181 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     6241 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     6301 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     6361 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     6421 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     6481 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     6541 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     6601 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     6661 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     6721 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     6781 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     6841 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     6901 aatgtattta gaaaaataaa caaatagggg ttccgcgcac a
//
