LOCUS       sequence-391642-       10171 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus expressing eGFP and Rabies Glycoprotein (G) from CVS-N2c 
            strain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10171)
  AUTHORS   Lee H, Ciabatti E, Gonzalez-Rueda A, Williams E, Nugent F, Mookerjee
            S, Morgese F, Tripodi M
  TITLE     Combining long-term circuit mapping and network transcriptomics with
            SiR-N2c.
  JOURNAL   Nat Methods. 2023 Apr;20(4):580-589. doi: 
            10.1038/s41592-023-01787-1. Epub 2023 Mar 2.
  PUBMED    36864202
REFERENCE   2  (bases 1 to 10171)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-023-01787-1"; journalName: "Nat Methods"; date: 
            "2023-04"; volume: "20"; issue: "4"; pages: "580-589"
COMMENT     Sequence Label: pLenti-CMV-GFP-2A-G(N2c)-WPRE
FEATURES             Location/Qualifiers
     source          1..10171
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        170..373
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     intron          522..900
                     /label=chimeric intron
                     /note="fusion between human cytomegalovirus intron A and 
                     human beta-globin intron 2"
     CDS             join(1008..1010,1011..1721)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(1050..1071)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1311..1330)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1658..1679
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1731..1796
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     misc_feature    3423..4011
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3476..3496)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3894..3905)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4098..4331
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4403..4524
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4440..4459)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4494..4513
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4564..4699
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4719..4738)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4720..4738)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4748..4765)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4748..4764)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4757..4779)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4906..5361
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4993..5012)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5203..5224
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5387..5491
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5492..5560,5561..6352)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5710..5729)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6523..7111
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7012..7031
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7265..7282
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7399..7420
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7435..7440,7441..7458,7459..7465)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7473..7489
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7478..7500
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7497..7513
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7497..7513
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7532..7552
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7534..7552
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7898..8101
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             8116..8296
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8343..8468
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8961..9194
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9379..9423
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9690..9807
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        join(10037..10171,1..169)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
       61 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
      121 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
      181 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
      241 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
      301 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
      361 tataagcaga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt
      421 tgacctccat agaagacacc gggaccgatc cagcctccgc ggattcgaat cccggccggg
      481 aacggtgcat tggaacgcgg attccccgtg ccaagagtga cgtaagtacc gcctatagag
      541 tctataggcc cacaaaaaat gctttcttct tttaatatac ttttttgttt atcttatttc
      601 taatactttc cctaatctct ttctttcagg gcaataatga tacaatgtat catgcctctt
      661 tgcaccattc taaagaataa cagtgataat ttctgggtta aggcaatagc aatatttctg
      721 catataaata tttctgcata taaattgtaa ctgatgtaag aggtttcata ttgctaatag
      781 cagctacaat ccagctacca ttctgctttt attttatggt tgggataagg ctggattatt
      841 ctgagtccaa gctaggccct tttgctaatc atgttcatac ctcttatctt cctcccacag
      901 ctcctgggca acgtgctggt ctgtgtgctg gcccatcact ttggcaaaga attgggattc
      961 gaacatcgat tgaattcggt acccttcagg tcgactccgg agccaccatg agcaagggcg
     1021 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
     1081 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
     1141 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
     1201 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
     1261 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
     1321 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     1381 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
     1441 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggccaact
     1501 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
     1561 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt
     1621 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
     1681 ccgccgccgg gatcactcac ggcatggacg agctgtacaa gggatccgga gtgaaacaga
     1741 ctttgaattt tgaccttctc aagttggcgg gagacgtgga gtccaaccca gggcccatgg
     1801 tgcctcaggt gctgctgttt gtgctgctgc tgggcttcag cctgtgcttc ggcaagttcc
     1861 ccatctacac catccccgac gagctgggcc cttggagccc tatcgatatc caccacctga
     1921 gctgccccaa caacctggtg gtggaagatg agggctgcac caacctgagc gagttcagct
     1981 acatggaact gaaagtgggc tacatcagcg ccatcaaagt gaacggcttc acctgtaccg
     2041 gcgtcgtgac cgaggccgag acatacacca acttcgtggg ctacgtgacc accaccttca
     2101 agagaaagca cttcagaccc acccccgacg cctgcagagc cgcctacaat tggaagatgg
     2161 ccggcgaccc cagatacgag gaaagcctgc acaaccccta ccccgactac cactggctga
     2221 gaaccgtgcg gaccaccaaa gagagcctga tcatcatcag ccccagcgtg accgacctgg
     2281 acccctacga caagagcctg cactccagag tgttccctgg cggcaagtgc agcggcatca
     2341 ccgtgtctag cacctactgc agcaccaacc acgactacac aatctggatg cccgagaacc
     2401 ccagacccag aaccccctgc gacatcttca ccaacagcag aggcaagaga gccagcaacg
     2461 gcaacaagac ctgcggcttc gtggacgaga gaggcctgta caagtccctg aagggcgcct
     2521 gccggctgaa gctgtgtggc gtgctgggac tgagactgat ggacggcaca tgggtggcca
     2581 tgcagaccag cgacgagaca aagtggtgcc cccctgatca gctcgtgaac ctgcacgact
     2641 tcagatccga cgagatcgag catctggtgg tggaagaact cgtgaagaaa agggaagagt
     2701 gcctggacgc cctggaatcc atcatgacca ccaagagcgt gtccttcaga aggctgagcc
     2761 acctgagaaa gctggtgccc ggctttggca aggcttacac catcttcaac aagacactga
     2821 tggaagccga cgcccactac aagtctgtgc ggacctggaa cgagatcatc cccagcaagg
     2881 gctgcctgaa agtgggaggc agatgccacc ctcacgtgaa cggcgtgttc ttcaacggca
     2941 tcatcctggg ccccgacgac cacgtgctga tccctgagat gcagagcagc ctgctgcagc
     3001 agcatatgga actgctggaa agcagcgtga tccccctgat gcaccctctg gccgacccta
     3061 gcaccgtgtt caaagagggc gacgaggctg aggacttcgt ggaagtgcat ctgcccgacg
     3121 tgtacaagca gatcagcggc gtggacctgg gcctgcccaa ctggggcaaa tatgtgctga
     3181 tgaccgctgg cgccatgatc ggcctggtgc tgatcttctc cctgatgacc tggtgcagaa
     3241 gggccaacag acccgagagc aagcagagaa gcttcggcgg cacaggcggc aacgtgtccg
     3301 tgacaagcca gagcggcaaa gtgatcccta gctgggagag ctacaagagc ggcggagaga
     3361 tcagactgtg atgatgaaag cttgcctcga ggatggggac tgataaaagc ttcgagctcg
     3421 acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     3481 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     3541 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     3601 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     3661 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     3721 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     3781 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc
     3841 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     3901 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     3961 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaattc
     4021 gagctcggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     4081 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt
     4141 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     4201 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     4261 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     4321 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
     4381 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag
     4441 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     4501 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg
     4561 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     4621 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     4681 ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg
     4741 cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     4801 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     4861 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg
     4921 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     4981 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     5041 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     5101 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     5161 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     5221 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     5281 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     5341 aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat
     5401 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     5461 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     5521 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     5581 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     5641 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     5701 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     5761 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     5821 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     5881 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     5941 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     6001 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     6061 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     6121 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     6181 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     6241 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     6301 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     6361 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     6421 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     6481 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     6541 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     6601 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     6661 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     6721 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     6781 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     6841 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     6901 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     6961 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     7021 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     7081 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     7141 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     7201 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     7261 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     7321 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     7381 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     7441 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
     7501 aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa
     7561 gctggagctg caagcttggc cattgcatac gttgtatcca tatcataata tgtacattta
     7621 tattggctca tgtccaacan taccsccrys ttgacattga ttatngacnt rkktwyymat
     7681 agtaayvmmw wwvgggryyt tmmrttsamg tcaatgggtg gagtatttac ggtaaactgc
     7741 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     7801 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     7861 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
     7921 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
     7981 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
     8041 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
     8101 tcgtttagtg aaccggggtc tctctggtta gaccagatct gagcctggga gctctctggc
     8161 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     8221 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     8281 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg
     8341 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
     8401 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg
     8461 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc
     8521 cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac
     8581 gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac
     8641 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacagtag
     8701 caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca
     8761 agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc
     8821 agacctggag gaggagatat gagggacaat tggagaagtg aattatataa atataaagta
     8881 gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga
     8941 gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc
     9001 actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata
     9061 gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc
     9121 acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag
     9181 gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg
     9241 ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg
     9301 atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa
     9361 tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt
     9421 ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata
     9481 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
     9541 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac
     9601 aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta
     9661 gtgaacggat ctcgacggta tcggttaact tttaaaagaa aaggggggat tggggggtac
     9721 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
     9781 aaacaaatta caaaaattca aaattttatc gatcacgaga ctagcctcga ctctagaaag
     9841 acgcgtctag tyaytaawrg kaaymaawkm yggrgyyryw agytyrkmsm yyryataygk
     9901 wgtntccryr tyayaatayk tacatttata ttgsctcatg tccaacatta ccgccatgty
     9961 kmcattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
    10021 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
    10081 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
    10141 ctttccattg acgtcaatgg gtggagtatt t
//
