LOCUS       sequence-391646-       10428 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus expressing eGFP and (EnvA-N2c Rabies Glycoprotein) fusion
            protein. Used to package EnvA pseudotyped G-deleted Rabies of 
            CVS-N2c strain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10428)
  AUTHORS   Lee H, Ciabatti E, Gonzalez-Rueda A, Williams E, Nugent F, Mookerjee
            S, Morgese F, Tripodi M
  TITLE     Combining long-term circuit mapping and network transcriptomics with
            SiR-N2c.
  JOURNAL   Nat Methods. 2023 Apr;20(4):580-589. doi: 
            10.1038/s41592-023-01787-1. Epub 2023 Mar 2.
  PUBMED    36864202
REFERENCE   2  (bases 1 to 10428)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-023-01787-1"; journalName: "Nat Methods"; date: 
            "2023-04"; volume: "20"; issue: "4"; pages: "580-589"
COMMENT     Sequence Label: pLenti-CMV-CAG-GFP-EnvA(N2c)
FEATURES             Location/Qualifiers
     source          1..10428
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     intron          733..1111
                     /label=chimeric intron
                     /note="fusion between human cytomegalovirus intron A and 
                     human beta-globin intron 2"
     CDS             join(1219..1221,1222..1932)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(1261..1282)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1522..1541)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1869..1890
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1942..2007
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     misc_feature    3892..4480
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3945..3965)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4363..4374)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4567..4800
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4872..4993
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4909..4928)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4963..4982
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5033..5168
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5188..5207)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5189..5207)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5217..5234)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5217..5233)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5226..5248)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5375..5830
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5462..5481)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5672..5693
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5856..5960
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5961..6029,6030..6821)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6179..6198)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6992..7580
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7481..7500
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7734..7751
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7868..7889
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7904..7909,7910..7927,7928..7934)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7942..7958
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7947..7969
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7966..7982
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7966..7982
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8001..8021
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8003..8021
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8366..8569
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             8584..8764
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8811..8936
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9429..9662
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9847..9891
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10158..10275
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      661 ccagcctccg cggattcgaa tcccggccgg gaacggtgca ttggaacgcg gattccccgt
      721 gccaagagtg acgtaagtac cgcctataga gtctataggc ccacaaaaaa tgctttcttc
      781 ttttaatata cttttttgtt tatcttattt ctaatacttt ccctaatctc tttctttcag
      841 ggcaataatg atacaatgta tcatgcctct ttgcaccatt ctaaagaata acagtgataa
      901 tttctgggtt aaggcaatag caatatttct gcatataaat atttctgcat ataaattgta
      961 actgatgtaa gaggtttcat attgctaata gcagctacaa tccagctacc attctgcttt
     1021 tattttatgg ttgggataag gctggattat tctgagtcca agctaggccc ttttgctaat
     1081 catgttcata cctcttatct tcctcccaca gctcctgggc aacgtgctgg tctgtgtgct
     1141 ggcccatcac tttggcaaag aattgggatt cgaacatcga ttgaattcgg tacccttcag
     1201 gtcgactccg gagccaccat gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     1261 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
     1321 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     1381 gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac
     1441 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     1501 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     1561 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     1621 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
     1681 gacaagcaga agaacggcat caaggccaac ttcaagatcc gccacaacat cgaggacggc
     1741 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     1801 ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag
     1861 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactca cggcatggac
     1921 gagctgtaca agggatccgg agtgaaacag actttgaatt ttgaccttct caagttggcg
     1981 ggagacgtgg agtccaaccc agggcccatg gaagccgtca taaaggcatt tctgactggg
     2041 caccctggaa aggtgagcaa gaaggactcc aagaagaagc cgccagcaac aagcaagaaa
     2101 gacccggaga agacgccctt gctgccaacg agagttaatt atattattgg tgtcctggtc
     2161 ttgtgtgagg taacgggggt aagagctgat gtccacttac tcgagcagcc agggaacctt
     2221 tggattacat gggccaaccg tacaggccaa acggattttt gcctctctac acagtcagcc
     2281 acctcccctt ttcaaacatg tttgataggt atcccgtccc ctatttccga gggtgatttt
     2341 aagggatatg tttctgatac aaattgcacc accttgggaa ctgatcggtt agtctcgtca
     2401 gccgacttta ctggcggacc tgacaacagt accaccctca cttatcggaa ggtctcatgc
     2461 ttgttgttaa agctgaatgt ctctatgtgg gatgagccac ctgaactaca gctgttaggt
     2521 tcccagtctc tccctaacat tactaatatt gctcagattt ccggtataac cgggggatgc
     2581 gtaggcttca gaccacaagg ggttccttgg tatctaggtt ggtctagaca ggaggccacg
     2641 cggtttctcc ttagacaccc ctctttctct aaatccacgg aaccgtttac ggtggtgaca
     2701 gcggataggc acaatctttt tatggggagt gagtactgcg gtgcatatgg ctacagattt
     2761 tggaacatgt ataactgctc acaggtgggg cggcagtacc gctgtggtaa tgcgcgcagc
     2821 ccccgcccgg gtcttcctga aatccagtgt acaaggagag gaggcaaatg ggttaatcaa
     2881 tcacaggaaa ttaatgagtc ggagccgttc agctttacgg tgaactgtac agctagtagt
     2941 ttgggtaatg ccagtgggtg ttgcggaaaa gcaggcacga ttctcccggg aaagtgggtc
     3001 gacagcacac aaggtagttt caccaaacca aaagcgctac cacccgcaat tttcctcatt
     3061 tgtggggatc gcgcatggca aggaattccc agtcgtccgg tagggggccc ctgctattta
     3121 ggcaagctta ccatgttagc acctaagcat acagatattc tcaaggtgct tgtcaattca
     3181 tcgcggacag gtataagacg taaacgaagc acctcacacc tggatgatac atgctcagat
     3241 gaagtgcagc tttggggtcc tacagcaaga atctttgcat ctatcctagc cccgggggta
     3301 gcagctgcgc aagccttaag agaaattgag agactagcct gttggtccgt taaacaggct
     3361 aacttgacaa catcactcct cggggactta ttggatgatg tcacgagtat tcgacacgcg
     3421 gtcctgcaga accgagcggc tattgacttc ttgctcctag ctcacggcca tggctgtgag
     3481 gacgttgccg gaatgtgctg tttcaatttg agtgatcaca gtgagtctat acagaagaag
     3541 ttccagctaa tgaaggaaca tgtcaataag atcggcgtgg acagcgaccc aattggaagt
     3601 tggctgcgag gactattcgg gggaatagga gaatgggccg ttcatttgct gaaaggactg
     3661 cttttggggc ttgtagttat tttgttgcta gtagtgtgcc tgccttgcag aagagccaat
     3721 cgaccagaat cgaaacaacg cagttttgga gggacagggg ggaatgtgtc agtcacttcc
     3781 caaagcggaa aagtcatacc ttcatgggaa tcatataaga gtggaggtga gatcagactg
     3841 taataaaagc ttgcctcgag gatggggact gataaaagct tcgagctcga caatcaacct
     3901 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     3961 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     4021 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     4081 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     4141 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     4201 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     4261 gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt
     4321 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     4381 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     4441 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaattcg agctcggtac
     4501 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     4561 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
     4621 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     4681 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     4741 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     4801 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     4861 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     4921 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     4981 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc
     5041 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     5101 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     5161 tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
     5221 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     5281 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     5341 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
     5401 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     5461 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     5521 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     5581 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     5641 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     5701 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     5761 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     5821 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     5881 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     5941 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     6001 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     6061 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     6121 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     6181 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     6241 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     6301 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     6361 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     6421 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     6481 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     6541 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     6601 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     6661 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     6721 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     6781 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     6841 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     6901 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     6961 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     7021 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     7081 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     7141 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     7201 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     7261 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     7321 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     7381 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     7441 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     7501 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     7561 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     7621 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     7681 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     7741 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     7801 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     7861 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     7921 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     7981 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc
     8041 aagcttggcc attgcatacg ttgtatccat atcataatat gtacatttat attggctcat
     8101 gtccaacatt accgccatgt tgacattgat tattgactak ttwtyaatag taacsmcaat
     8161 mgggrcyttm mrttsacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     8221 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     8281 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     8341 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
     8401 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
     8461 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
     8521 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc gtttagtgaa
     8581 ccggggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     8641 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     8701 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     8761 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
     8821 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
     8881 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
     8941 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     9001 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     9061 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     9121 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     9181 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     9241 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga
     9301 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
     9361 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
     9421 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
     9481 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
     9541 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
     9601 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
     9661 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
     9721 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
     9781 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
     9841 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
     9901 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
     9961 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
    10021 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
    10081 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct
    10141 cgacggtatc ggttaacttt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
    10201 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
    10261 aaaattcaaa attttatcga tcacgagact agcctcgact ctagaaagac gcgtctagtt
    10321 attaatagta atcaattayg grgyyrytag ytyrtmgmcy ryataygtwg tntccatatc
    10381 ataatatgta catttatatt ggctcatgtc caacattacc gccatgtt
//
