LOCUS       sequence-391660-        5213 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lysosomal associated membrane protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5213)
  AUTHORS   Nava S, Palma W, Wan X, Oh JY, Gharib S, Wang H, Revanna JS, Tan M, 
            Zhang M, Liu J, Chen CH, Lee JS, Perry B, Sternberg PW
  TITLE     A cGAL-UAS bipartite expression toolkit for Caenorhabditis elegans 
            sensory neurons.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Dec 19;120(51):e2221680120. doi: 
            10.1073/pnas.2221680120. Epub 2023 Dec 14.
  PUBMED    38096407
REFERENCE   2  (bases 1 to 5213)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-12-19"; volume: "120(51):e2221680120. 
            doi"; pages: " 10.1073/pnas.2221680120. Epub 2023 Dec 14"
COMMENT     Sequence Label: pJR026
FEATURES             Location/Qualifiers
     source          1..5213
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    36..130
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    169..263
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    302..396
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     primer_bind     complement(1588..1616)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2364..2384
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     promoter        2958..3062
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3063..3131,3132..3923)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3281..3300)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4094..4682
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4583..4602
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4836..4853
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4970..4991
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5006..5011,5012..5029,5030..5036)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5044..5060
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5049..5071
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5068..5084
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5068..5084
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcta gcccatgatt acgccaagct acgggcggag tactgtcctc cgagcggagt
       61 actgtcctcc gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact
      121 gtcctccgag cggagactcc ctagcccatg attacgccaa gctacgggcg gagtactgtc
      181 ctccgagcgg agtactgtcc tccgagcgga gtactgtcct ccgagcggag tactgtcctc
      241 cgagcggagt actgtcctcc gagcggagac tccctagccc atgattacgc caagctacgg
      301 gcggagtact gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg
      361 gagtactgtc ctccgagcgg agtactgtcc tccgagcgga gactccctag gggcggccgc
      421 actgactggg ccggccaaaa atgcataagg ttttgctggc actgttcttt atctttctgg
      481 caccagcatc cgcactggca gtctccgaac cggcctgcag gtcgattttt tgcaaattac
      541 gagcgttgta gggggcggag cgataggtcc tataggtttt ggtatatcat cattcattca
      601 ttcattggta cattcattta cccaccttcc tctttctgag cttctctgga gttctgtgct
      661 tcctttttcc cttatcttta tactgtaatt tttaactttc aggcattgat tggcgcgcct
      721 ctagaggatc cccgggattg gccaaaggac ccaaaggtat gtttcgaatg atactaacat
      781 aacatagaac attttcagga ggacccttgg ctagcgtcga cggtaccggt agaaaaaatg
      841 ttgaaatcgt ttgtcatctt gtttgccttc ctggcctccg catccgccac cgcttcgcat
      901 tactatgtaa ccaacaacaa caccggactc acgtgcatca tcctcgacgg agacttccag
      961 ttcaatttgg tcttcaacga gaagaacacc accgagaagt tcacggtcac attcaacgag
     1021 acagtcagtg tcgaaggaga ttgcaacgga gtccgcaaca atcaatcggt tcagacgttg
     1081 aatattaagt tcaaccctga aggacaatct gcccgttacg ccaaagagtg ggagctcgat
     1141 atcgtgttcg gatcatctag caacgtcgcc tttgagatca ttgactacac cctcaccact
     1201 cagagaacgg atcttgtccc atactttgga aagtttgtca gagacgccaa tgctgccgga
     1261 gatgttacag caactcagac caacgcttac aagtgctcca ccgccaagct tggactcgtc
     1321 ggaggatcta ccattgacat caagacatcc aacgtgattg catttgcaca gatgaacggc
     1381 accgttttcc caactgacca agtctacgag gtgtgctatt tggatgctcg caccaacgaa
     1441 gttgtcccaa tcgtcgtcgg agcctgcctc gcggctctcg tcgtggttgt gctcgtcgga
     1501 taccttatcg gacgtgctcg tgcaaagaga caaggatatg ccagcgtcgt accaaaaatg
     1561 agtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat
     1621 gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa
     1681 cttaccctta aattaatttg cactactgga aaactacctg ttccatgggt aagtttaaac
     1741 atatatatac taactaaccc tgattattta aattttcagc caacacttgt cactacttta
     1801 ggttatggtc ttcaatgctt cgccagatac ccagatcata tgaaacggca tgactttttc
     1861 aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg
     1921 aactacaaga cacgtaagtt taaacagttc ggtactaact aaccatacat atttaaattt
     1981 tcaggtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga gttaaaaggt
     2041 attgatttta aagaagatgg aaacattctt ggacacaaat tggaatacaa ctataactca
     2101 cacaatgtat acatcactgc cgacaaacaa aagaatggga tcaaagcggt aagtttaaac
     2161 atgattttac taactaacta atctgattta aattttcaga acttcaaaat tagacacaac
     2221 attgaagatg gaggagttca actagcagac cattatcaac aaaatactcc aattggcgat
     2281 ggccctgtcc ttttaccaga caaccattac ctgtcctatc aatctgccct ttcgaaagat
     2341 cccaacgaaa agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca
     2401 cttggcatgg atgaactata caaatagcat tcgtagaatt cgctagccgg ccatacaagt
     2461 aatccggatg atcgacgcca acgtcgttga attttcaaat tttaaatact gaatatttgt
     2521 tttttttcct attatttatt tattctcttt gtgttttttt tcttgctttc taaaaaatta
     2581 attcaatcca aatctaaaca tttttttttc tctttccgtc tcccaattcg tattccgctc
     2641 ctctcatctg aacacaatgt gcaagtttat ttatcttctc gctttcattt cattaggacg
     2701 tggggggaat tggtggaagg gggaaacaca caaaaggatg atggaaatga aataaggaca
     2761 cacaatatgc aacaacattc aattcagaaa tatggaggaa ggtttaaaag aaaacataaa
     2821 aatatataga ggaggaagga aaactagtaa aaaataagca aagaaattag gcgaacgatg
     2881 agaattgtcc tcgcttgggc ccaaaaggcc tttttttttt ggcgccgacg tcaggtggca
     2941 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     3001 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     3061 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     3121 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     3181 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     3241 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     3301 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     3361 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     3421 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     3481 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     3541 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     3601 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     3661 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     3721 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     3781 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     3841 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     3901 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     3961 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     4021 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     4081 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     4141 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     4201 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     4261 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     4321 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     4381 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     4441 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     4501 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     4561 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     4621 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     4681 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     4741 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     4801 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     4861 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     4921 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     4981 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     5041 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
     5101 tgtaagttta aacatgatct tactaactaa ctattctcat ttaaattttc agagcttaaa
     5161 aatggctgaa atcactcaca acgatggata cgctaacaac ttggaaatga aat
//
