LOCUS       sequence-391666-        3575 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Co-expression plasmid for the FLP, PVD neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   Nava S, Palma W, Wan X, Oh JY, Gharib S, Wang H, Revanna JS, Tan M, 
            Zhang M, Liu J, Chen CH, Lee JS, Perry B, Sternberg PW
  TITLE     A cGAL-UAS bipartite expression toolkit for Caenorhabditis elegans 
            sensory neurons.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Dec 19;120(51):e2221680120. doi: 
            10.1073/pnas.2221680120. Epub 2023 Dec 14.
  PUBMED    38096407
REFERENCE   2  (bases 1 to 3575)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-12-19"; volume: "120(51):e2221680120. 
            doi"; pages: " 10.1073/pnas.2221680120. Epub 2023 Dec 14"
COMMENT     Sequence Label: pWP170
FEATURES             Location/Qualifiers
     source          1..3575
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             633..782
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     promoter        1320..1424
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1425..1493,1494..2285)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1643..1662)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2456..3044
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2945..2964
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3198..3215
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3332..3353
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3368..3373,3374..3391,3392..3398)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3406..3422
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3411..3433
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3430..3446
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3430..3446
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcttggattt ttggcacttt ttattggcgc caaatttttt ttcggtaaaa
       61 tgcattttaa ggttaaattt aaaaacgtga tccctgatgt cttagttctc caaaaaaaaa
      121 acactttttt ttttcgaaaa ccttttagaa attgatcact tcagtggtgt gtcccccctt
      181 ttgtttgtca gtttttttta aggaaaaatc aatcaaaaaa gggtacaata aatgttaaat
      241 gagacatgtg tgtacctata taaatacgaa atcgaggttt gtgaaaaatt tcaaattttc
      301 cgagaaaaag ttttttccca aaaaaaaggc gcgcctctag aggatccccg ggattggcca
      361 aaggacccaa aggtatgttt cgaatgatac taacataaca tagaacattt tcaggaggac
      421 ccttggctag gatgggccct aaaaagaagc gtaaagctag catgatgttg aagaagattc
      481 tcaaaattga agaactggat gagcgtgagc tcatcgacat cgaggtctcc ggaaaccacc
      541 tcttctacgc caacgacatc ctcacccaca actcctcctc cgatgtactg cagtctggtg
      601 gcggaggggc tcctaggggt tccggacggg ctgacgcatt ggacgatttt gatctggata
      661 tgctgggaag tgacgccctc gatgattttg accttgacat gcttggttcg gatgcccttg
      721 atgactttga cctcgacatg ctcggcagtg acgcccttga tgatttcgac ctggacatgc
      781 tgattaacta aggtaccgaa ttcgctagcc ggccatacaa gtaatccgga tgatcgacgc
      841 caacgtcgtt gaattttcaa attttaaata ctgaatattt gttttttttc ctattattta
      901 tttattctct ttgtgttttt tttcttgctt tctaaaaaat taattcaatc caaatctaaa
      961 catttttttt tctctttccg tctcccaatt cgtattccgc tcctctcatc tgaacacaat
     1021 gtgcaagttt atttatcttc tcgctttcat ttcattagga cgtgggggga attggtggaa
     1081 gggggaaaca cacaaaagga tgatggaaat gaaataagga cacacaatat gcaacaacat
     1141 tcaattcaga aatatggagg aaggtttaaa agaaaacata aaaatatata gaggaggaag
     1201 gaaaactagt aaaaaataag caaagaaatt aggcgaacga tgagaattgt cctcgcttgg
     1261 gcccaaaagg cctttttttt ttggcgccga cgtcaggtgg cacttttcgg ggaaatgtgc
     1321 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     1381 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     1441 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     1501 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     1561 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     1621 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     1681 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     1741 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     1801 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     1861 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     1921 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     1981 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     2041 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     2101 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     2161 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     2221 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     2281 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     2341 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     2401 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     2461 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     2521 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     2581 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     2641 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     2701 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     2761 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     2821 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     2881 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     2941 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     3001 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     3061 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     3121 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     3181 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     3241 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     3301 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     3361 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     3421 aatttcacac aggaaacagc tatgaccatg attacgccaa gctgtaagtt taaacatgat
     3481 cttactaact aactattctc atttaaattt tcagagctta aaaatggctg aaatcactca
     3541 caacgatgga tacgctaaca acttggaaat gaaat
//
