LOCUS       sequence-391675-        4611 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Drives specific expression in the ASJ neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4611)
  AUTHORS   Nava S, Palma W, Wan X, Oh JY, Gharib S, Wang H, Revanna JS, Tan M, 
            Zhang M, Liu J, Chen CH, Lee JS, Perry B, Sternberg PW
  TITLE     A cGAL-UAS bipartite expression toolkit for Caenorhabditis elegans 
            sensory neurons.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Dec 19;120(51):e2221680120. doi: 
            10.1073/pnas.2221680120. Epub 2023 Dec 14.
  PUBMED    38096407
REFERENCE   2  (bases 1 to 4611)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-12-19"; volume: "120(51):e2221680120. 
            doi"; pages: " 10.1073/pnas.2221680120. Epub 2023 Dec 14"
COMMENT     Sequence Label: pCC14
FEATURES             Location/Qualifiers
     source          1..4611
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1669..1818
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     promoter        2356..2460
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2461..2529,2530..3321)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2679..2698)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3492..4080
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3981..4000
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4234..4251
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4368..4389
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4404..4409,4410..4427,4428..4434)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4442..4458
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4447..4469
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4466..4482
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4466..4482
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcgcggccgc actgactggg ccggccagaa gaactttttg ttcgtggagt
       61 tccaaatttt gaccatgatt ttgagtcgga agagatgact ttgaagacaa cattggatgg
      121 aaagaactat aataatcagt tcttgaatct aggaagaaag gagattattg gaaaacatca
      181 ttcaatggaa tatttcattt ctggaagaac tttgattaga ttggtaaaga acgaaggcca
      241 agaactatca cgtacagaat attatctgaa acatggagat ttgcatgtat atgacaaaag
      301 aggaaactta gattgtaccc gagtatatcg ttctcctacc atctctccta tgcttcaatc
      361 caaaattcgt caattttaat tttcatttgt gacagagttt tgcctaatga tattctatta
      421 gtccccgatt cttattttta atgtaaaatt ctgttttttt tttttcgctt tccatgttta
      481 aatatgtaca gtttttctat ttttcttttc aataaaaacc aacaacaaac atttgaaaat
      541 gcatctctgt ccaattttga gttgaagtga atagggcgca ttactgtgta accccactaa
      601 caattttaag gaaaattcat aactaaaaga gaaaatttta aaaaattaat ttaacaagca
      661 tatttcaaga ccaaactccc attgacgaaa ctttgctgac gacattatct gatttttagt
      721 tttcattttt gagcactgta attattttct tcattcttca gttttttttc tcagccttca
      781 ttattcatcc atgcctcctc ctcccacaca cctttttctg tttatcgatg cttcttcccc
      841 cgttcatcaa cccatgaatt aagtgggcgt gtacctgtcc gatcgactga aagtcttttt
      901 ctcttcatct tctcacttat ttcatataaa tatcatactg agactgactc agtttttatt
      961 tgaattttct acacttgtat ttcatcatgt ctctcaccaa ggagcctatt ctggagcttg
     1021 ctgatatggt aacagaggcg cgcctctaga ggatccccgg gattggccaa aggacccaaa
     1081 ggtatgtttc gaatgatact aacataacat agaacatttt caggaggacc cttggctagg
     1141 atgggcccta aaaagaagcg taaagctagc atgaagctgt tgtcttcaat ggaacaagct
     1201 tgcgacatat gccggttaaa aaaactcagg tgctcgaagg aaaagcccaa gtgtgccaag
     1261 tgtctgaaga acaactcgga gtgccgttat tctcccaagg ctatgaggtc cccactgacc
     1321 agggcgcatc tgacagaggt agaatcaagg ttggaaaaat tggaagattt gtttctgctc
     1381 atgtttcctc gggaaaacct ggacagcatt ttgaacatgg actctttgga cgaagtgaaa
     1441 gtaatgttga aacaattata tatgcaggac tatggtgaca cggatgctgc tatggatagc
     1501 ttgaccccta tggaaaatgg catgtctgaa gggccgagaa aacacatgaa gactgcgaca
     1561 tcatcgccaa aagtggatga agatggaagc caaagtcagt tgagtgtatc gccggaattc
     1621 ccgctgcagt ctggtggcgg aggggctcct aggggttccg gacgggctga cgcattggac
     1681 gattttgatc tggatatgct gggaagtgac gccctcgatg attttgacct tgacatgctt
     1741 ggttcggatg cccttgatga ctttgacctc gacatgctcg gcagtgacgc ccttgatgat
     1801 ttcgacctgg acatgctgat taactaaggt accgaattcg ctagccggcc atacaagtaa
     1861 tccggatgat cgacgccaac gtcgttgaat tttcaaattt taaatactga atatttgttt
     1921 tttttcctat tatttattta ttctctttgt gttttttttc ttgctttcta aaaaattaat
     1981 tcaatccaaa tctaaacatt tttttttctc tttccgtctc ccaattcgta ttccgctcct
     2041 ctcatctgaa cacaatgtgc aagtttattt atcttctcgc tttcatttca ttaggacgtg
     2101 gggggaattg gtggaagggg gaaacacaca aaaggatgat ggaaatgaaa taaggacaca
     2161 caatatgcaa caacattcaa ttcagaaata tggaggaagg tttaaaagaa aacataaaaa
     2221 tatatagagg aggaaggaaa actagtaaaa aataagcaaa gaaattaggc gaacgatgag
     2281 aattgtcctc gcttgggccc aaaaggcctt ttttttttgg cgccgacgtc aggtggtact
     2341 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     2401 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     2461 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     2521 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     2581 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     2641 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     2701 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     2761 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     2821 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     2881 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     2941 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     3001 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     3061 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     3121 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     3181 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     3241 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     3301 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     3361 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     3421 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     3481 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     3541 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     3601 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     3661 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     3721 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     3781 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     3841 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     3901 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     3961 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     4021 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     4081 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     4141 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     4201 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     4261 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     4321 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     4381 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     4441 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagctg
     4501 taagtttaaa catgatctta ctaactaact attctcattt aaattttcag agcttaaaaa
     4561 tggctgaaat cactcacaac gatggatacg ctaacaactt ggaaatgaaa t
//
