LOCUS       sequence-391680-        4322 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C. elegans optimized version of mScarlet.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4322)
  AUTHORS   Nava S, Palma W, Wan X, Oh JY, Gharib S, Wang H, Revanna JS, Tan M, 
            Zhang M, Liu J, Chen CH, Lee JS, Perry B, Sternberg PW
  TITLE     A cGAL-UAS bipartite expression toolkit for Caenorhabditis elegans 
            sensory neurons.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Dec 19;120(51):e2221680120. doi: 
            10.1073/pnas.2221680120. Epub 2023 Dec 14.
  PUBMED    38096407
REFERENCE   2  (bases 1 to 4322)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-12-19"; volume: "120(51):e2221680120. 
            doi"; pages: " 10.1073/pnas.2221680120. Epub 2023 Dec 14"
COMMENT     Sequence Label: pJR022
FEATURES             Location/Qualifiers
     source          1..4322
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    36..130
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    169..263
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    302..396
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        2067..2171
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2172..2240,2241..3032)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2390..2409)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3203..3791
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3692..3711
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3945..3962
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4079..4100
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4115..4120,4121..4138,4139..4145)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4153..4169
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4158..4180
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4177..4193
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4177..4193
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcta gcccatgatt acgccaagct acgggcggag tactgtcctc cgagcggagt
       61 actgtcctcc gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact
      121 gtcctccgag cggagactcc ctagcccatg attacgccaa gctacgggcg gagtactgtc
      181 ctccgagcgg agtactgtcc tccgagcgga gtactgtcct ccgagcggag tactgtcctc
      241 cgagcggagt actgtcctcc gagcggagac tccctagccc atgattacgc caagctacgg
      301 gcggagtact gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg
      361 gagtactgtc ctccgagcgg agtactgtcc tccgagcgga gactccctag gggcggccgc
      421 actgactggg ccggccaaaa atgcataagg ttttgctggc actgttcttt atctttctgg
      481 caccagcatc cgcactggca gtctccgaac cggcctgcag gtcgattttt tgcaaattac
      541 gagcgttgta gggggcggag cgataggtcc tataggtttt ggtatatcat cattcattca
      601 ttcattggta cattcattta cccaccttcc tctttctgag cttctctgga gttctgtgct
      661 tcctttttcc cttatcttta tactgtaatt tttaactttc aggcattgat tggcgcgcct
      721 ctagaggatc cccgggattg gccaaaggac ccaaaggtat gtttcgaatg atactaacat
      781 aacatagaac attttcagga ggacccttgg ctagcgtcga cggtaccggt agaaaaaatg
      841 gtcagcaagg gagaggcagt tatcaaggag ttcatgcgtt tcaaggtcca catggaggga
      901 tccatgaacg gacacgagtt cgagatcgag ggagagggag agggacgtcc atacgaggga
      961 acccaaaccg ccaagctcaa ggtcaccaag ggaggaccac tcccattctc ctgggacatc
     1021 ctctccccac aattcatgta cggatcccgt gccttcacca agcacccagc cgacatccca
     1081 gactactaca agcaatcctt cccagaggga ttcaagtggg agcgtgtcat gaacttcgag
     1141 gacggaggag ccgtcaccgt cacccaagac acctccctcg aggacggaac cctcatctac
     1201 aaggtcaagc tccgtggaac caacttccca ccagacggac cagtcatgca aaagaagacc
     1261 atgggatggg aggcctccac cgagcgtctc tacccagagg acggagtcct caagggagac
     1321 atcaagatgg ccctccgtct caaggacgga ggacgttacc tcgccgactt caagaccacc
     1381 tacaaggcca agaagccagt ccaaatgcca ggagcctaca acgtcgaccg taagctcgac
     1441 atcacctccc acaacgagga ctacaccgtc gtcgagcaat acgagcgttc cgagggacgt
     1501 cactccaccg gaggaatgga cgagctctac aagtaacatt cgtagaattc gctagccggc
     1561 catacaagta atccggatga tcgacgccaa cgtcgttgaa ttttcaaatt ttaaatactg
     1621 aatatttgtt ttttttccta ttatttattt attctctttg tgtttttttt cttgctttct
     1681 aaaaaattaa ttcaatccaa atctaaacat ttttttttct ctttccgtct cccaattcgt
     1741 attccgctcc tctcatctga acacaatgtg caagtttatt tatcttctcg ctttcatttc
     1801 attaggacgt ggggggaatt ggtggaaggg ggaaacacac aaaaggatga tggaaatgaa
     1861 ataaggacac acaatatgca acaacattca attcagaaat atggaggaag gtttaaaaga
     1921 aaacataaaa atatatagag gaggaaggaa aactagtaaa aaataagcaa agaaattagg
     1981 cgaacgatga gaattgtcct cgcttgggcc caaaaggcct tttttttttg gcgccgacgt
     2041 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     2101 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     2161 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     2221 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     2281 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     2341 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     2401 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     2461 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     2521 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     2581 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     2641 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     2701 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     2761 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     2821 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     2881 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     2941 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     3001 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     3061 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     3121 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     3181 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     3241 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     3301 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     3361 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     3421 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     3481 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     3541 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     3601 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     3661 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     3721 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     3781 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     3841 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     3901 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     3961 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     4021 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     4081 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     4141 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     4201 acgccaagct gtaagtttaa acatgatctt actaactaac tattctcatt taaattttca
     4261 gagcttaaaa atggctgaaa tcactcacaa cgatggatac gctaacaact tggaaatgaa
     4321 at
//
