LOCUS       sequence-391683-        5623 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Natural light-gated anion channels. In vivo inhibition of neurons 
            using blue light. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5623)
  AUTHORS   Nava S, Palma W, Wan X, Oh JY, Gharib S, Wang H, Revanna JS, Tan M, 
            Zhang M, Liu J, Chen CH, Lee JS, Perry B, Sternberg PW
  TITLE     A cGAL-UAS bipartite expression toolkit for Caenorhabditis elegans 
            sensory neurons.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Dec 19;120(51):e2221680120. doi: 
            10.1073/pnas.2221680120. Epub 2023 Dec 14.
  PUBMED    38096407
REFERENCE   2  (bases 1 to 5623)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-12-19"; volume: "120(51):e2221680120. 
            doi"; pages: " 10.1073/pnas.2221680120. Epub 2023 Dec 14"
COMMENT     Sequence Label: pJR014
FEATURES             Location/Qualifiers
     source          1..5623
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    36..130
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    169..263
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    302..396
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     primer_bind     complement(2006..2034)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2782..2802
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     promoter        3368..3472
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3473..3541,3542..4333)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3691..3710)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4504..5092
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4993..5012
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5246..5263
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5380..5401
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5416..5421,5422..5439,5440..5446)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5454..5470
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5459..5481
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5478..5494
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5478..5494
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcta gcccatgatt acgccaagct acgggcggag tactgtcctc cgagcggagt
       61 actgtcctcc gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact
      121 gtcctccgag cggagactcc ctagcccatg attacgccaa gctacgggcg gagtactgtc
      181 ctccgagcgg agtactgtcc tccgagcgga gtactgtcct ccgagcggag tactgtcctc
      241 cgagcggagt actgtcctcc gagcggagac tccctagccc atgattacgc caagctacgg
      301 gcggagtact gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg
      361 gagtactgtc ctccgagcgg agtactgtcc tccgagcgga gactccctag gggcggccgc
      421 actgactggg ccggccaaaa atgcataagg ttttgctggc actgttcttt atctttctgg
      481 caccagcatc cgcactggca gtctccgaac cggcctgcag gtcgattttt tgcaaattac
      541 gagcgttgta gggggcggag cgataggtcc tataggtttt ggtatatcat cattcattca
      601 ttcattggta cattcattta cccaccttcc tctttctgag cttctctgga gttctgtgct
      661 tcctttttcc cttatcttta tactgtaatt tttaactttc aggcattgat tggcgcgcct
      721 ctagaggatc cccgggattg gccaaaggac ccaaaggtat gtttcgaatg atactaacat
      781 aacatagaac attttcagga ggacccttgg ctagcgtcga cggtaccggt aaaaatggca
      841 tcacaagttg tgtatggtga atgggcatca acacacacag agtgctacaa catgtcacga
      901 attgattcta cattcgtgtc attgcttcag ttggtctggg cagtcgtgtc aggatgccag
      961 acaatcttca tgatctcacg agctccgaaa gtcccatggg aatcagtgta ccttccattc
     1021 gttgagtcta tcacttacgc cttggcctca acaggaaatg gaactcttca gatgcgtgac
     1081 ggtcgattct tcccatggtc tcgaatggca tcatggttgt gcacttgccc aatcatgttg
     1141 ggtcaaatct ctaacatggc cttggtgaag tacaagtcaa tcccattgaa cccgattgcc
     1201 caggctgctt caatcattcg agtggtgatg ggtatcacag ccacaatctc tccagctgag
     1261 tacatgaagt ggttgttctt cttcttcggt gcaacttgtc ttgtcttcga gtactctgtg
     1321 gtgttcacaa tcttccaggt cggattgtac ggattcgagt ctgtcggaac accacttgcc
     1381 cagaaggtcg tggtgcgtat caagatgttg cgtcttatct tcttcatcgc ttggacaatg
     1441 ttcccgatcg tctggttgat ctcaccaact ggtgtctgcg tcatccacga gaatgtctct
     1501 gcaatccttt acttgcttgc cgacggactt tgcaagaata cttatggagt tatcttgtgg
     1561 tcaactgcct ggggtgtcct tgaaggtaaa tgggacccag cttgccttcc aggacaggag
     1621 aagccagaag ctgacgaccc gttcggattg aaccacgaga agaacgctcc accaaacgac
     1681 gaggtgaaca tccgaatgtt cggaagatga ggtaccgtag ctgtctcatc ctactttcac
     1741 ctagttaact gcttgtctta aaatctatgc ttctctttag tatctaaaat tttcctagaa
     1801 gcttacaagt atataaatgg tctcttctca ataaaggttg tatatttatt catcttattg
     1861 aatctgccat ttcctcgttt ttgcgagttt atataccttc caattttctt tctattgtat
     1921 tttcaacttc taattttaat tcagggaaac tgctgtaccg gtagaaaaaa tgctcgagag
     1981 taaaggagaa gaacttttca ctggagttgt cccaattctt gttgaattag atggtgatgt
     2041 taatgggcac aaattttctg tcagtggaga gggtgaaggt gatgcaacat acggaaaact
     2101 tacccttaaa tttatttgca ctactggaaa actacctgtt ccatgggtaa gtttaaacat
     2161 atatatacta actaaccctg attatttaaa ttttcagcca acacttgtca ctactttctg
     2221 ttatggtgtt caatgcttct cgagataccc agatcatatg aaacggcatg actttttcaa
     2281 gagtgccatg cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa
     2341 ctacaagaca cgtaagttta aacagttcgg tactaactaa ccatacatat ttaaattttc
     2401 aggtgctgaa gtcaagtttg aaggtgatac ccttgttaat agaatcgagt taaaaggtat
     2461 tgattttaaa gaagatggaa acattcttgg acacaaattg gaatacaact ataactcaca
     2521 caatgtatac atcatggcag acaaacaaaa gaatggaatc aaagttgtaa gtttaaacat
     2581 gattttacta actaactaat ctgatttaaa ttttcagaac ttcaaaatta gacacaacat
     2641 tgaagatgga agcgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg
     2701 ccctgtcctt ttaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc
     2761 caacgaaaag agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca
     2821 tggcatggat gaactataca aataggaatt cgctagccgg ccatacaagt aatccggatg
     2881 atcgacgcca acgtcgttga attttcaaat tttaaatact gaatatttgt tttttttcct
     2941 attatttatt tattctcttt gtgttttttt tcttgctttc taaaaaatta attcaatcca
     3001 aatctaaaca tttttttttc tctttccgtc tcccaattcg tattccgctc ctctcatctg
     3061 aacacaatgt gcaagtttat ttatcttctc gctttcattt cattaggacg tggggggaat
     3121 tggtggaagg gggaaacaca caaaaggatg atggaaatga aataaggaca cacaatatgc
     3181 aacaacattc aattcagaaa tatggaggaa ggtttaaaag aaaacataaa aatatataga
     3241 ggaggaagga aaactagtaa aaaataagca aagaaattag gcgaacgatg agaattgtcc
     3301 tcgcttgggc ccaaaaggcc tttttttttt ggcgccgacg tcaggtggca cttttcgggg
     3361 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     3421 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     3481 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     3541 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     3601 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     3661 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     3721 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     3781 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     3841 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     3901 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     3961 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     4021 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     4081 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     4141 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     4201 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     4261 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     4321 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     4381 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     4441 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     4501 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     4561 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     4621 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     4681 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     4741 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     4801 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     4861 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     4921 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     4981 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     5041 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     5101 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     5161 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     5221 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     5281 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     5341 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     5401 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     5461 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc tgtaagttta
     5521 aacatgatct tactaactaa ctattctcat ttaaattttc agagcttaaa aatggctgaa
     5581 atcactcaca acgatggata cgctaacaac ttggaaatga aat
//
