LOCUS       sequence-391688-        5236 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For tissue specific RNA-seq.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5236)
  AUTHORS   Nava S, Palma W, Wan X, Oh JY, Gharib S, Wang H, Revanna JS, Tan M, 
            Zhang M, Liu J, Chen CH, Lee JS, Perry B, Sternberg PW
  TITLE     A cGAL-UAS bipartite expression toolkit for Caenorhabditis elegans 
            sensory neurons.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Dec 19;120(51):e2221680120. doi: 
            10.1073/pnas.2221680120. Epub 2023 Dec 14.
  PUBMED    38096407
REFERENCE   2  (bases 1 to 5236)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-12-19"; volume: "120(51):e2221680120. 
            doi"; pages: " 10.1073/pnas.2221680120. Epub 2023 Dec 14"
COMMENT     Sequence Label: pJR035
FEATURES             Location/Qualifiers
     source          1..5236
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    36..130
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    169..263
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    302..396
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     CDS             1243..1332
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     promoter        2981..3085
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3086..3154,3155..3946)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3304..3323)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4117..4705
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4606..4625
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4859..4876
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4993..5014
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5029..5034,5035..5052,5053..5059)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5067..5083
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5072..5094
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5091..5107
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5091..5107
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcta gcccatgatt acgccaagct acgggcggag tactgtcctc cgagcggagt
       61 actgtcctcc gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact
      121 gtcctccgag cggagactcc ctagcccatg attacgccaa gctacgggcg gagtactgtc
      181 ctccgagcgg agtactgtcc tccgagcgga gtactgtcct ccgagcggag tactgtcctc
      241 cgagcggagt actgtcctcc gagcggagac tccctagccc atgattacgc caagctacgg
      301 gcggagtact gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg
      361 gagtactgtc ctccgagcgg agtactgtcc tccgagcgga gactccctag gggcggccgc
      421 actgactggg ccggccaaaa atgcataagg ttttgctggc actgttcttt atctttctgg
      481 caccagcatc cgcactggca gtctccgaac cggcctgcag gtcgattttt tgcaaattac
      541 gagcgttgta gggggcggag cgataggtcc tataggtttt ggtatatcat cattcattca
      601 ttcattggta cattcattta cccaccttcc tctttctgag cttctctgga gttctgtgct
      661 tcctttttcc cttatcttta tactgtaatt tttaactttc aggcattgat tggcgcgcct
      721 ctagaggatc cccgggattg gccaaaggac ccaaaggtat gtttcgaatg atactaacat
      781 aacatagaac attttcagga ggacccttgg ctagcgtcga cggtaccggt gcgatcgcta
      841 gcaaaaatgg tcccaaagcc acacgccaag tcggccaaga aggctcttcg caagaagaag
      901 gtgcacctca agttcaacgt tgagtgcaag aacccagttg aggatggaat tctcagaatc
      961 gaagatcttg aagccttctt gaacgagaag atcaaggtca atggaaagac cggacatttg
     1021 gccgctaaca acgtcaaggt cgaggtcgcc aagtccaagg tctcagtcgt ctccgaggtc
     1081 ccattctcga agagatacct caagtacctt accaagaagt acctcaagag aaactccctc
     1141 cgtgattggc tccgtgtggt cgctgtcaac aagaacacct acgaggtcag atacttccac
     1201 atcaacgacg gagaggacgc aggaagcgac cacgaggaat tctacccata cgatgttcct
     1261 gactatgcgg gctatcccta tgacgtcccg gactatgcag gatcctatcc atatgacgtt
     1321 ccagattacg ctgctcagta gcctgcaggt tttgaattcg ctgtctcatc ctactttcac
     1381 ctagttaact gcttgtctta aaatctatgc ttctctttag tatctaaaat tttcctagaa
     1441 gcttacaagt atataaatgg tctcttctca ataaaggttg tatatttatt catcttattg
     1501 aatctgccat ttcctcgttt ttgcgagttt atataccttc caattttctt tctattgtat
     1561 tttcaacttc taattttaat tcagggaaac tgctgtaccg gtagaaaaaa tggtctccga
     1621 gctcattaaa gaaaacatgc atatgaagct ctacatggag ggaaccgtca acaaccacca
     1681 cttcaagtgc acctccgagg gagagggaaa gccatacgag ggaacccaaa ccatgcgtat
     1741 caaggccgtc gagggaggac cactcccatt cgccttcgac atcctcgcca cctccttcat
     1801 gtacggatcc aaggtaagtt taaacatata tatactaact aaccctgatt atttaaattt
     1861 tcagaccttc atcaaccaca cccaaggaat cccagacttc ttcaagcaat ccttcccaga
     1921 gggattcacc tgggagcgtg tcaccaccta cgaggacgga ggagtcctca ccgccaccca
     1981 agacacctcc ctccaagacg gatgcctcat ctacaacgtc aaggtaagtt taaacagttc
     2041 ggtactaact aaccatacat atttaaattt tcagatccgt ggagtcaact tcccatccaa
     2101 cggaccagtc atgcaaaaga agaccctcgg atgggaggcc tccaccgaga ccctctaccc
     2161 agccgacgga ggacttgagg gacgtgccga catggccctc aagctcgtcg gaggaggaca
     2221 cctcatctgc aacctcaagg taagtttaaa catgatttta ctaactaact aatctgattt
     2281 aaattttcag accacctacc gttccaagaa gccagccaag aacctcaaga tgccaggagt
     2341 ctactacgtc gaccgtcgtc ttgagcgtat caaggaggcc gacaaggaga cctacgtcga
     2401 gcaacacgag gtcgccgtcg cccgttactg cgacctccca tccaagctcg gacaccgtta
     2461 attcgctagc cggccataca agtaatccgg atgatcgacg ccaacgtcgt tgaattttca
     2521 aattttaaat actgaatatt tgtttttttt cctattattt atttattctc tttgtgtttt
     2581 ttttcttgct ttctaaaaaa ttaattcaat ccaaatctaa acattttttt ttctctttcc
     2641 gtctcccaat tcgtattccg ctcctctcat ctgaacacaa tgtgcaagtt tatttatctt
     2701 ctcgctttca tttcattagg acgtgggggg aattggtgga agggggaaac acacaaaagg
     2761 atgatggaaa tgaaataagg acacacaata tgcaacaaca ttcaattcag aaatatggag
     2821 gaaggtttaa aagaaaacat aaaaatatat agaggaggaa ggaaaactag taaaaaataa
     2881 gcaaagaaat taggcgaacg atgagaattg tcctcgcttg ggcccaaaag gccttttttt
     2941 tttggcgccg acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     3001 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     3061 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     3121 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     3181 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     3241 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     3301 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     3361 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     3421 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     3481 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     3541 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     3601 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     3661 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     3721 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     3781 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     3841 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     3901 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     3961 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     4021 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     4081 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     4141 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     4201 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     4261 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     4321 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     4381 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     4441 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     4501 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     4561 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     4621 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     4681 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     4741 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     4801 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     4861 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     4921 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     4981 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     5041 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     5101 ctatgaccat gattacgcca agctgtaagt ttaaacatga tcttactaac taactattct
     5161 catttaaatt ttcagagctt aaaaatggct gaaatcactc acaacgatgg atacgctaac
     5221 aacttggaaa tgaaat
//
