LOCUS       sequence-391692-        6142 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Epidermal growth factor .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6142)
  AUTHORS   Nava S, Palma W, Wan X, Oh JY, Gharib S, Wang H, Revanna JS, Tan M, 
            Zhang M, Liu J, Chen CH, Lee JS, Perry B, Sternberg PW
  TITLE     A cGAL-UAS bipartite expression toolkit for Caenorhabditis elegans 
            sensory neurons.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Dec 19;120(51):e2221680120. doi: 
            10.1073/pnas.2221680120. Epub 2023 Dec 14.
  PUBMED    38096407
REFERENCE   2  (bases 1 to 6142)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-12-19"; volume: "120(51):e2221680120. 
            doi"; pages: " 10.1073/pnas.2221680120. Epub 2023 Dec 14"
COMMENT     Sequence Label: pJR015
FEATURES             Location/Qualifiers
     source          1..6142
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    36..130
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    169..263
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    302..396
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     primer_bind     complement(2525..2553)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     3301..3321
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     promoter        3887..3991
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3992..4060,4061..4852)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4210..4229)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5023..5611
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5512..5531
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5765..5782
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5899..5920
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5935..5940,5941..5958,5959..5965)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5973..5989
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5978..6000
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5997..6013
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5997..6013
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcta gcccatgatt acgccaagct acgggcggag tactgtcctc cgagcggagt
       61 actgtcctcc gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact
      121 gtcctccgag cggagactcc ctagcccatg attacgccaa gctacgggcg gagtactgtc
      181 ctccgagcgg agtactgtcc tccgagcgga gtactgtcct ccgagcggag tactgtcctc
      241 cgagcggagt actgtcctcc gagcggagac tccctagccc atgattacgc caagctacgg
      301 gcggagtact gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg
      361 gagtactgtc ctccgagcgg agtactgtcc tccgagcgga gactccctag gggcggccgc
      421 actgactggg ccggccaaaa atgcataagg ttttgctggc actgttcttt atctttctgg
      481 caccagcatc cgcactggca gtctccgaac cggcctgcag gtcgattttt tgcaaattac
      541 gagcgttgta gggggcggag cgataggtcc tataggtttt ggtatatcat cattcattca
      601 ttcattggta cattcattta cccaccttcc tctttctgag cttctctgga gttctgtgct
      661 tcctttttcc cttatcttta tactgtaatt tttaactttc aggcattgat tggcgcgcct
      721 ctagaggatc cccgggattg gccaaaggac ccaaaggtat gtttcgaatg atactaacat
      781 aacatagaac attttcagga ggacccttgg ctagcgtcga cggtaccggt aaaaatgcgg
      841 aaaatgctac ttttttgcat ccttctactc tttatgcctc aatttacagt ttcagaatcg
      901 tgtctccctt cgtggtttcg tcaagaacgt agtgctcccg aacagcttca atctgcagag
      961 aatgcagctg aaaatagtgg ctctgtacca cccgatactt ctcgaaattc tctagaaaca
     1021 aacgaaatag gtgatgcacc gtcgtcgact tcgacacctg aaacacctac tgaaactacg
     1081 atttccgaag ctggagacga tgaaaaacga actgaagagg ttgcaaaaga attaatcgag
     1141 aaagaagcag aatatgaggg tgaatatgaa gatgaaaagg ttgatgaaga agtagaagaa
     1201 gcgttaaaat ataatgaaga tgccactcaa gatgccacgt cgactcttaa accggcggtt
     1261 cggaaggaaa tcgagaagtt gaaagaagca aaatgcaaag actactgtca tcacaacgcg
     1321 acatgccacg tggaagtgat attccgtgaa gatagagttt cagcagttgt tccttcttgc
     1381 cattgtccac agggttggga aggcactcgt tgtgatcgtc actacgttca ggcgttctat
     1441 gccccaatca acggcagata taatggaaaa acgaaaaaac cattaatttt tatggttcat
     1501 catccaaatc aaacgatttc tacaacgcct tccagtcaag attctgaaat ttcttctatt
     1561 ttcagtgggc tttatgagag aattgtgcaa tcttcaacat cagctattcc tgcgttcgca
     1621 tttctcattg tcatgctcat catgtttata acaattgttg tttatgctta tagaagaatg
     1681 tctaaacgat cggatgatat gacatataca atgagtcata tgtgcccacc agaagcattc
     1741 aatgtcctca aaacaccaaa tggacgacat attccagttc atcaaattcc atcatgttct
     1801 tatactatcc caacaccggg tacagtacct ccaaatatat catcaactcc tggatcaaga
     1861 atacccactc gtcaacaagc tattcgaaat aatgaacaag cacggaacaa ctttttcagc
     1921 attctcagaa gtcaaggtac cattccatcc aggagtatca atgacgacga cacgccgaag
     1981 cactacaagt cagtgccacg tgttgaagtt tcagcaatta attactctgg ccacattgat
     2041 ttttcaacag tatcatatca gtcgactgaa tcagaagttt caaaagcatc agtaacatgt
     2101 ccaccaccgg cgcacactgt aattaatatc gagttggatt ctgcagatac gaattttcga
     2161 tccccgtctc gaagttctgg agaacaagga tcaccagcaa catgtgaacc aatgattcga
     2221 cacacatgag gtaccgtagc tgtctcatcc tactttcacc tagttaactg cttgtcttaa
     2281 aatctatgct tctctttagt atctaaaatt ttcctagaag cttacaagta tataaatggt
     2341 ctcttctcaa taaaggttgt atatttattc atcttattga atctgccatt tcctcgtttt
     2401 tgcgagttta tataccttcc aattttcttt ctattgtatt ttcaacttct aattttaatt
     2461 cagggaaact gctgtaccgg tagaaaaaat gctcgagagt aaaggagaag aacttttcac
     2521 tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt
     2581 cagtggagag ggtgaaggtg atgcaacata cggaaaactt acccttaaat ttatttgcac
     2641 tactggaaaa ctacctgttc catgggtaag tttaaacata tatatactaa ctaaccctga
     2701 ttatttaaat tttcagccaa cacttgtcac tactttctgt tatggtgttc aatgcttctc
     2761 gagataccca gatcatatga aacggcatga ctttttcaag agtgccatgc ccgaaggtta
     2821 tgtacaggaa agaactatat ttttcaaaga tgacgggaac tacaagacac gtaagtttaa
     2881 acagttcggt actaactaac catacatatt taaattttca ggtgctgaag tcaagtttga
     2941 aggtgatacc cttgttaata gaatcgagtt aaaaggtatt gattttaaag aagatggaaa
     3001 cattcttgga cacaaattgg aatacaacta taactcacac aatgtataca tcatggcaga
     3061 caaacaaaag aatggaatca aagttgtaag tttaaacatg attttactaa ctaactaatc
     3121 tgatttaaat tttcagaact tcaaaattag acacaacatt gaagatggaa gcgttcaact
     3181 agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa
     3241 ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga gagaccacat
     3301 ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa
     3361 ataggaattc gctagccggc catacaagta atccggatga tcgacgccaa cgtcgttgaa
     3421 ttttcaaatt ttaaatactg aatatttgtt ttttttccta ttatttattt attctctttg
     3481 tgtttttttt cttgctttct aaaaaattaa ttcaatccaa atctaaacat ttttttttct
     3541 ctttccgtct cccaattcgt attccgctcc tctcatctga acacaatgtg caagtttatt
     3601 tatcttctcg ctttcatttc attaggacgt ggggggaatt ggtggaaggg ggaaacacac
     3661 aaaaggatga tggaaatgaa ataaggacac acaatatgca acaacattca attcagaaat
     3721 atggaggaag gtttaaaaga aaacataaaa atatatagag gaggaaggaa aactagtaaa
     3781 aaataagcaa agaaattagg cgaacgatga gaattgtcct cgcttgggcc caaaaggcct
     3841 tttttttttg gcgccgacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     3901 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     3961 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     4021 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     4081 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     4141 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     4201 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     4261 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     4321 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     4381 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     4441 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     4501 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     4561 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     4621 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     4681 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     4741 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     4801 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     4861 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     4921 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     4981 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     5041 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     5101 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     5161 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     5221 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     5281 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     5341 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     5401 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     5461 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     5521 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     5581 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     5641 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     5701 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     5761 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     5821 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     5881 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     5941 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
     6001 aaacagctat gaccatgatt acgccaagct gtaagtttaa acatgatctt actaactaac
     6061 tattctcatt taaattttca gagcttaaaa atggctgaaa tcactcacaa cgatggatac
     6121 gctaacaact tggaaatgaa at
//
