LOCUS       sequence-391696-        5630 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Actin filament and cell cortex localization..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5630)
  AUTHORS   Nava S, Palma W, Wan X, Oh JY, Gharib S, Wang H, Revanna JS, Tan M, 
            Zhang M, Liu J, Chen CH, Lee JS, Perry B, Sternberg PW
  TITLE     A cGAL-UAS bipartite expression toolkit for Caenorhabditis elegans 
            sensory neurons.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Dec 19;120(51):e2221680120. doi: 
            10.1073/pnas.2221680120. Epub 2023 Dec 14.
  PUBMED    38096407
REFERENCE   2  (bases 1 to 5630)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-12-19"; volume: "120(51):e2221680120. 
            doi"; pages: " 10.1073/pnas.2221680120. Epub 2023 Dec 14"
COMMENT     Sequence Label: pJR025
FEATURES             Location/Qualifiers
     source          1..5630
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    36..130
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    169..263
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    302..396
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     primer_bind     complement(2005..2033)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2781..2801
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     promoter        3375..3479
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3480..3548,3549..4340)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3698..3717)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4511..5099
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5000..5019
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5253..5270
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5387..5408
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5423..5428,5429..5446,5447..5453)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5461..5477
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5466..5488
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5485..5501
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5485..5501
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcta gcccatgatt acgccaagct acgggcggag tactgtcctc cgagcggagt
       61 actgtcctcc gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact
      121 gtcctccgag cggagactcc ctagcccatg attacgccaa gctacgggcg gagtactgtc
      181 ctccgagcgg agtactgtcc tccgagcgga gtactgtcct ccgagcggag tactgtcctc
      241 cgagcggagt actgtcctcc gagcggagac tccctagccc atgattacgc caagctacgg
      301 gcggagtact gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg
      361 gagtactgtc ctccgagcgg agtactgtcc tccgagcgga gactccctag gggcggccgc
      421 actgactggg ccggccaaaa atgcataagg ttttgctggc actgttcttt atctttctgg
      481 caccagcatc cgcactggca gtctccgaac cggcctgcag gtcgattttt tgcaaattac
      541 gagcgttgta gggggcggag cgataggtcc tataggtttt ggtatatcat cattcattca
      601 ttcattggta cattcattta cccaccttcc tctttctgag cttctctgga gttctgtgct
      661 tcctttttcc cttatcttta tactgtaatt tttaactttc aggcattgat tggcgcgcct
      721 ctagaggatc cccgggattg gccaaaggac ccaaaggtat gtttcgaatg atactaacat
      781 aacatagaac attttcagga ggacccttgg ctagcgtcga cggtaccggt agaaaaaatg
      841 tgtgacgacg atgttgccgc tctcgtagtt gacaatggat ccggaatgtg caaagctgga
      901 ttcgctggag acgacgctcc acgcgccgtg ttcccatcca ttgtcggaag acctcgtcat
      961 caaggagtca tggtcggtat gggacagaaa gactcgtacg tcggagacga ggcccaatcc
     1021 aagagaggta tccttaccct taagtaccca attgagcatg gtatcgttac caactgggac
     1081 gacatggaaa aaatctggca tcacactttc tacaacgagc ttcgcgttgc cccagaagag
     1141 cacccagtac ttctcactga agccccactc aatccaaagg ctaaccgtga aaagatgacc
     1201 caaatcatgt tcgagacctt caatacccca gccatgtatg tcgccatcca agctgtcctc
     1261 tccctctacg cttccggacg taccaccgga atcgtcctcg actctggaga tggtgttacc
     1321 cacacagtcc caatctacga aggatatgcc ctcccacacg ccatcctccg tcttgacttg
     1381 gctggacgtg atcttactga ttacctcatg aagatcctta ccgagcgtgg ttactctttc
     1441 accaccaccg ctgagcgtga aatcgtccgt gacatcaagg agaagctttg ttacgtcgcc
     1501 ctcgatttcg agcaagaaat ggccaccgcc gcttcttcct cttccctcga gaagtcctac
     1561 gaacttcctg acggacaagt catcactgtt ggaaacgaac gcttccgttg cccagaggct
     1621 ctgttccaac catccttctt gggtatggaa tctgcaggaa tccacgagac ttcttacaac
     1681 tccatcatga agtgcgacat tgatatccgt aaggacttgt acgccaacac tgttctttcc
     1741 ggaggaacca ccatgtaccc aggaattgct gatcgtatgc agaaggaaat caccgctctt
     1801 gccccatcaa ccatgaagat caagatcatc gccccaccag agcgcaagta ctccgtctgg
     1861 atcggaggat ctatcctcgc ctccctctcc accttccaac agatgtggat ctccaagcaa
     1921 gaatacgacg agtctggacc atcaattgtt catcgcaaat gcttcgtacc aaaaatgagt
     1981 aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt
     2041 aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt
     2101 acccttaaat taatttgcac tactggaaaa ctacctgttc catgggtaag tttaaacata
     2161 tatatactaa ctaaccctga ttatttaaat tttcagccaa cacttgtcac tactttaggt
     2221 tatggtcttc aatgcttcgc cagataccca gatcatatga aacggcatga ctttttcaag
     2281 agtgccatgc ccgaaggtta tgtacaggaa agaactatat ttttcaaaga tgacgggaac
     2341 tacaagacac gtaagtttaa acagttcggt actaactaac catacatatt taaattttca
     2401 ggtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt aaaaggtatt
     2461 gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta taactcacac
     2521 aatgtataca tcactgccga caaacaaaag aatgggatca aagcggtaag tttaaacatg
     2581 attttactaa ctaactaatc tgatttaaat tttcagaact tcaaaattag acacaacatt
     2641 gaagatggag gagttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc
     2701 cctgtccttt taccagacaa ccattacctg tcctatcaat ctgccctttc gaaagatccc
     2761 aacgaaaaga gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacactt
     2821 ggcatggatg aactatacaa atagcattcg tagaattcgc tagccggcca tacaagtaat
     2881 ccggatgatc gacgccaacg tcgttgaatt ttcaaatttt aaatactgaa tatttgtttt
     2941 ttttcctatt atttatttat tctctttgtg ttttttttct tgctttctaa aaaattaatt
     3001 caatccaaat ctaaacattt ttttttctct ttccgtctcc caattcgtat tccgctcctc
     3061 tcatctgaac acaatgtgca agtttattta tcttctcgct ttcatttcat taggacgtgg
     3121 ggggaattgg tggaaggggg aaacacacaa aaggatgatg gaaatgaaat aaggacacac
     3181 aatatgcaac aacattcaat tcagaaatat ggaggaaggt ttaaaagaaa acataaaaat
     3241 atatagagga ggaaggaaaa ctagtaaaaa ataagcaaag aaattaggcg aacgatgaga
     3301 attgtcctcg cttgggccca aaaggccttt tttttttggc gccgacgtca ggtggcactt
     3361 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     3421 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     3481 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     3541 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     3601 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     3661 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     3721 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     3781 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     3841 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     3901 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     3961 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     4021 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     4081 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     4141 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     4201 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     4261 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     4321 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     4381 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     4441 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     4501 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     4561 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     4621 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     4681 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     4741 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     4801 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     4861 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     4921 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     4981 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     5041 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     5101 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     5161 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     5221 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     5281 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     5341 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     5401 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     5461 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctgt
     5521 aagtttaaac atgatcttac taactaacta ttctcattta aattttcaga gcttaaaaat
     5581 ggctgaaatc actcacaacg atggatacgc taacaacttg gaaatgaaat
//
