LOCUS       sequence-391720-        4927 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  enhancer AAV for Cre recombinase expression in striatal medium spiny
            neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4927)
  AUTHORS   Hunker AC, Wirthlin ME, Gill G, Johansen NJ, Hooper M, Omstead V, 
            Vargas S, Lerma MN, Taskin N, Weed N, Laird WD, Bishaw YM, Bendrick 
            JL, Gore BB, Ben-Simon Y, Opitz-Araya X, Martinez RA, Way SW, 
            Thyagarajan B, Otto S, Sanchez REA, Alexander JR, Amaya A, Amster A,
            Arbuckle J, Ayala A, Baker PM, Barcelli T, Barta S, Bertagnolli D, 
            Bielstein C, Bishwakarma P, Bowlus J, Boyer G, Brouner K, Casian B, 
            Casper T, Chakka AB, Chakrabarty R, Chong P, Clark M, Colbert K, 
            Daniel S, Dawe T, Departee M, DiValentin P, Donadio NP, Dotson NI, 
            Dwivedi D, Egdorf T, Fliss T, Gary A, Goldy J, Grasso C, Groce EL, 
            Gudsnuk K, Han W, Haradon Z, Hastings S, Helback O, Ho WV, Huang C, 
            Johnson T, Jones DL, Juneau Z, Kenney J, Leibly M, Li S, Liang E, 
            Loeffler H, Lusk NA, Madigan Z, Malloy J, Malone J, McCue R, Melchor
            J, Mich JK, Moosman S, Morin E, Naidoo R, Newman D, Ngo K, Nguyen K,
            Oster AL, Ouellette B, Oyama AA, Pena N, Pham T, Phillips E, Pom C, 
            Potekhina L, Ransford S, Ray PL, Reding M, Rette DF, Reynoldson C, 
            Rimorin C, Sigler AR, Rocha DB, Ronellenfitch K, Ruiz A, Sawyer L, 
            Sevigny JP, Shapovalova NV, Shepard N, Shulga L, Soliman S, Staats 
            B, Taormina MJ, Tieu M, Wang Y, Wilkes J, Wood T, Zhou T, Williford 
            A, Dee N, Mollenkopf T, Ng L, Esposito L, Kalmbach BE, Yao S, Ariza 
            J, Collman F, Mufti S, Smith K, Waters J, Ersing I, Patrick M, Zeng 
            H, Lein ES, Kojima Y, Horwitz G, Owen SF, Levi BP, Daigle TL, Tasic 
            B, Bakken TE, Ting JT
  TITLE     Enhancer AAV toolbox for accessing and perturbing striatal cell 
            types and circuits.
  JOURNAL   Neuron. 2025 May 21;113(10):1507-1524.e17. doi: 
            10.1016/j.neuron.2025.04.035.
  PUBMED    40403704
REFERENCE   2  (bases 1 to 4927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2025.04"; journalName: "Neuron"; date: 
            "2025-05-21- 21"; volume: "113"; issue: "10"; pages: "1507-1524.e17"
COMMENT     Sequence Label: AiP13778 - pAAV-AiE0450h-minBG-iCre(R297T)-BGH...
FEATURES             Location/Qualifiers
     source          1..4927
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     regulatory      863..872
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             875..895
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1961..2072
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   2190..2330
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2190..2319
                     /label=AAV2 ITR
     rep_origin      2405..2860
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2492..2511)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2702..2723
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2877..2896)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2996..3018
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3056..3074)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3142..3246
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3247..3315,3316..4107)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3465..3484)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4278..4866
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4767..4786
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgagacc acaggctgag aggccaggca gggttcacca
      181 ggacactctg aaagagctgt ctggctccca ggcccacagg ccttcgaggg aatagacaca
      241 taagccttgc tcctttttct cctggtatct gcccacaccc atatcctgga aagctgcgtg
      301 atttatgtcc accagagggg atttgctctg aggctgtggc ctgcaggagc gcccttccgc
      361 tggagggtgg gaggactgtc taaggcagtc acagcaagga gaggcacggt gtgggcactg
      421 ctgctatttc taacctgctg gagtgaaggg aaccttgact acttgaaaag caggctgatt
      481 gtcaggggcg gaaccgaact cagagctgtc agggccgtca catggaaagt gcctggcagg
      541 gggcggcgac agggggcttg ggctgacagg gctgtgattt tgaaagaaac gtctccaaga
      601 aagtgattta aagtaggaga gaaatattat tattagataa tggctgtcag gttatttttt
      661 gatgtgtttt ctgagtattt cataatcatc ttgcgcttag atggtaagcc cttcctggct
      721 aggggccgtg tcccatccca tgcctctgtg gctttcacct tcgggtgtgc aacaggtatc
      781 agagctcggg ctgggcataa aagtcagggc agagccatct attgcttaca tttgcttctg
      841 ggatccagat ctttcgaagc tagccaccat ggtgcccaag aagaagagga aagtctccaa
      901 cctgctgact gtgcaccaaa acctgcctgc cctccctgtg gatgccacct ctgatgaagt
      961 caggaagaac ctgatggaca tgttcaggga caggcaggcc ttctctgaac acacctggaa
     1021 gatgctcctg tctgtgtgca gatcctgggc tgcctggtgc aagctgaaca acaggaaatg
     1081 gttccctgct gaacctgagg atgtgaggga ctacctcctg tacctgcaag ccagaggcct
     1141 ggctgtgaag accatccaac agcacctggg ccagctcaac atgctgcaca ggagatctgg
     1201 cctgcctcgc ccttctgact ccaatgctgt gtccctggtg atgaggagaa tcagaaagga
     1261 gaatgtggat gctggggaga gagccaagca ggccctggcc tttgaacgca ctgactttga
     1321 ccaagtcaga tccctgatgg agaactctga cagatgccag gacatcagga acctggcctt
     1381 cctgggcatt gcctacaaca ccctgctgcg cattgccgaa attgccagaa tcagagtgaa
     1441 ggacatctcc cgcaccgatg gtgggagaat gctgatccac attggcagga ccaagaccct
     1501 ggtgtccaca gctggtgtgg agaaggccct gtccctgggg gttaccaagc tggtggagag
     1561 atggatctct gtgtctggtg tggctgatga ccccaacaac tacctgttct gccgggtcag
     1621 aaagaatggt gtggctgccc cttctgccac ctcccaactg tccacccggg ccctggaagg
     1681 gatctttgag gccacccacc gcctgatcta tggtgccaag gatgactctg ggcagagata
     1741 cctggcctgg tctggccact ctgccagagt gggtgctgcc accgacatgg ccagggctgg
     1801 tgtgtccatc cctgaaatca tgcaggctgg tggctggacc aatgtgaaca ttgtgatgaa
     1861 ctacatcaga aacctggact ctgagactgg ggccatggtg aggctgctcg aagatgggga
     1921 ctgaggcgcg ccgaattcaa gcttctcgag agatcttcga ctgtgccttc tagttgccag
     1981 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     2041 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     2101 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     2161 gagatctcac gtgcggaccg agcggccgca ggaaccccta gtgatggagt tggccactcc
     2221 ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg
     2281 ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat
     2341 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca
     2401 tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     2461 accgctacac ttgccagcgc cttagcgccc gctcctttcg ctttcttccc ttcctttctc
     2521 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     2581 tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt
     2641 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     2701 agtggactct tgttccaaac tggaacaaca ctcaactcta tctcgggcta ttcttttgat
     2761 ttataaggga ttttgccgat ttcggtctat tggttaaaaa atgagctgat ttaacaaaaa
     2821 tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca
     2881 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     2941 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     3001 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
     3061 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     3121 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     3181 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     3241 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     3301 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     3361 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     3421 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     3481 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     3541 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     3601 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     3661 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     3721 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     3781 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     3841 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     3901 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     3961 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     4021 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     4081 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     4141 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     4201 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     4261 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     4321 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     4381 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     4441 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     4501 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     4561 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     4621 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     4681 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     4741 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     4801 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     4861 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     4921 cacatgt
//
