LOCUS       sequence-39173-e         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Billings NA, Emerson MM, Cepko CL
  TITLE     Analysis of thyroid response element activity during retinal 
            development.
  JOURNAL   PLoS One. 2010  . 5(10):e13739.
  PUBMED    21060789
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2010  . 5(10):e13739."
COMMENT     Sequence Label: Stagia3
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(193..297)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    370..418
                     /note="synthetic polyadenylation signal"
     misc_feature    432..523
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     472..491
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     regulatory      633..642
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(684..705)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ggaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
       61 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
      121 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
      181 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
      241 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
      301 atttccccga aaagtgccac ctgggtcgag aagtaatatt aaggtacggg aggtacttgg
      361 agcggccgca ataaaatatc tttattttca ttacatctgt gtgttggttt tttgtgtgaa
      421 tcgatagtac taacatacgc tctccatcaa aacaaaacga aacaaaacaa actagcaaaa
      481 taggctgtcc ccagtgcaag tgcaggtgcc agaacatttc tctatcgata gtcgaccaat
      541 tgctcgagga attctatata atggaagctt gaccggagcc gttagttgcc cgtcggacgc
      601 gatttgcgag gcgctttatg cgcgcaccgg tcgccaccat ggtgagcaag ggcgaggagc
      661 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
      721 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
      781 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg
      841 gcgtgcagtg cttcagccg
//
