LOCUS       sequence-391732-        9099 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for expression of KSHV ORF29 with G226A and E321K 
            mutation .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9099)
  AUTHORS   McCollum CO, Didychuk AL, Liu D, Murray-Nerger LA, Cristea IM, 
            Glaunsinger BA
  TITLE     The viral packaging motor potentiates Kaposi's sarcoma-associated 
            herpesvirus gene expression late in infection.
  JOURNAL   PLoS Pathog. 2023 Apr 17;19(4):e1011163. doi: 
            10.1371/journal.ppat.1011163. eCollection 2023 Apr.
  PUBMED    37068108
REFERENCE   2  (bases 1 to 9099)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog."; date: "2023-04-17"; volume: "19(4):e1011163. doi"; pages: 
            " 10.1371/journal.ppat.1011163. eCollection 2023 Apr"
COMMENT     Sequence Label: pLJM1-zeo G226A E321K ORF29
FEATURES             Location/Qualifiers
     source          1..9099
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        131..334
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     285..305
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    2476..2593
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2642..3152
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3071..3089
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             3161..3535
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     LTR             3607..3840
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3912..4033
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3949..3968)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4003..4022
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4073..4208
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4228..4247)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4229..4247)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4257..4274)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4257..4273)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4266..4288)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4415..4870
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4502..4521)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4712..4733
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4896..5000
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5001..5069,5070..5861)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5219..5238)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6032..6620
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6521..6540
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6774..6791
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6908..6929
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6944..6949,6950..6967,6968..6974)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6982..6998
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6987..7009
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7006..7022
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7006..7022
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7041..7061
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7043..7061
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7089..7315
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7316..7496
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7543..7668
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8161..8394
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8579..8623
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     8921..8941
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
       61 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      121 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      181 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      241 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      301 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      361 gcgctaccgg tatgctgctc agccgtcaca gggagcgcct tgccgccaac ctgcaggaga
      421 ccgccaaaga cgccggagag aggtgggaac tgagtgcccc gacattcacg cgacactgtc
      481 ccaaaacggc acggatggcg caccctttta ttggcgtggt gcacagaata aactcataca
      541 gttcggtcct ggaaacatac tgcacacggc accatcccgc cacgcccacg tcagcaaatc
      601 ccgacgtggg aacccccaga ccgtccgagg acaacgtccc cgcaaagccg cgcctattgg
      661 agtccctatc aacatacttg cagatgcggt gtgtgcgcga ggacgcgcac gtctccacgg
      721 ccgatcaact ggtcgagtac caggcggcca gaaaaacaca cgactccctg cacgcctgct
      781 ctgtctaccg cgaacttcag gcttttctgg ttaacctttc gtcctttctg aacggctgtt
      841 acgttcccgg ggtgcactgg ctggagccct tccaacagca gctagtaatg cacacttttt
      901 tctttttggt ttcaatcaag gccccacaaa agacgcacca gttgtttgga ttgtttaagc
      961 agtacttcgg tttatttgaa actccaaaca gtgttttaca gacgtttaag caaaaggcaa
     1021 gcgtattcct aataccaagg agacacgcta agacatggat agtggtggcg atcatcagca
     1081 tgctactggc atccgtagag aacattaaca ttgggtacgt agcccaccaa aagcacgtag
     1141 ccaactccgt gttcgcggaa atcataaaga cgctttgtcg gtggttcccc cccaaaaatt
     1201 taaacatcaa gaaggagaac ggaaccataa tctacacgcg acccggagga cggtccagct
     1261 cgctgatgtg cgcaacatgc ttcaataaga acagcattcg gggacagacc tttaacctgc
     1321 tctacgtaga caaggcgaat tttattaaaa aggatgcact gccggctatt ctgggtttca
     1381 tgcttcagaa agacgccaag ctgatattta tatcatccgt gaactcgtca gaccgctcca
     1441 cgagtttcct gcttaacctc aggaacgccc aggaaaagat gctgaatgtg gtcagttacg
     1501 tgtgtgcgga ccaccgcgaa gatttccacc tgcaagacgc actagtgtcc tgtccttgtt
     1561 acagactgca cattccgacg tacatcacca tcgacgaatc catcaaaacc accaccaacc
     1621 tctttatgga gggggcattc gacaccgaac taatgggcga gggagcagcg tcgtcaaatg
     1681 ctacgcttta ccgcgtggtg ggtgacgcag cgctgacaca gtttgacatg tgtcgggtag
     1741 acaccaccgc ccagcaggtt cagaagtgcc ttggaaaaca gctgtttgtt tacatcgacc
     1801 ccgcgtatac gaacaacacg gaggcgtccg gtactggcgt gggcgccgtt gtcacgagta
     1861 ctcagactcc caccagaagc ctcatattgg gcatggagca tttcttcctg cgcgacctca
     1921 ctggcgcagc tgcttacgag atagcgtcct gcgcatgcac gatgattaag gcgatcgctg
     1981 tgctccaccc cacaattgag cgcgtgaacg cggcggtcga aggcaacagc agccaagatt
     2041 ctggggtggc tattgcaacc gtccttaacg aaatatgccc gctccccata cattttctac
     2101 actatactga caagagcagc gccctgcagt ggccaattta catgctggga ggcgagaaat
     2161 cctccgcgtt tgagacattc atctacgctc tgaactccgg caccctgagc gccagccaga
     2221 cggtggtgtc caacaccatc aaaatatcat ttgacccggt gacctacctg gtagaacagg
     2281 tccgcgcgat caagtgcgtc ccgcttaggg atggagggca gtcatacagc gccaagcaaa
     2341 agcacatgtc ggacgactta cttgtggcag ttgtcatggc ccattttatg gctaccgatg
     2401 atagacacat gtacaagccc atatccccac aataaaattc tcgacctcga gacaaatggc
     2461 agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
     2521 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
     2581 aattcaaaat tttcgggttt attacaggga cagcagagat ccactttggc cgcggctcga
     2641 gggggttggg gttgcgcctt ttccaaggca gccctgggtt tgcgcaggga cgcggctgct
     2701 ctgggcgtgg ttccgggaaa cgcagcggcg ccgaccctgg gtctcgcaca ttcttcacgt
     2761 ccgttcgcag cgtcacccgg atcttcgccg ctacccttgt gggccccccg gcgacgcttc
     2821 ctgctccgcc cctaagtcgg gaaggttcct tgcggttcgc ggcgtgccgg acgtgacaaa
     2881 cggaagccgc acgtctcact agtaccctcg cagacggaca gcgccaggga gcaatggcag
     2941 cgcgccgacc gcgatgggct gtggccaata gcggctgctc agcagggcgc gccgagagca
     3001 gcggccggga aggggcggtg cgggaggcgg ggtgtggggc ggtagtgtgg gccctgttcc
     3061 tgcccgcgcg gtgttccgca ttctgcaagc ctccggagcg cacgtcggca gtcggctccc
     3121 tcgttgaccg aatcaccgac ctctctcccc agggggatcc atggccaagt tgaccagtgc
     3181 cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct
     3241 cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac
     3301 cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg
     3361 ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg
     3421 ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc
     3481 cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgaggtac
     3541 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     3601 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
     3661 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     3721 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     3781 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     3841 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     3901 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     3961 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     4021 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc
     4081 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     4141 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     4201 tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
     4261 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     4321 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     4381 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
     4441 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     4501 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     4561 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     4621 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     4681 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     4741 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     4801 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     4861 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     4921 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     4981 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     5041 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     5101 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     5161 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     5221 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     5281 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     5341 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     5401 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     5461 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     5521 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     5581 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     5641 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     5701 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     5761 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     5821 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     5881 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     5941 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     6001 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     6061 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     6121 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     6181 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     6241 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     6301 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     6361 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     6421 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     6481 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     6541 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     6601 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     6661 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     6721 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     6781 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     6841 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     6901 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     6961 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     7021 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc
     7081 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta
     7141 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg
     7201 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac
     7261 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc
     7321 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     7381 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     7441 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
     7501 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
     7561 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
     7621 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
     7681 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
     7741 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
     7801 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
     7861 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
     7921 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
     7981 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat
     8041 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     8101 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     8161 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     8221 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     8281 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     8341 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     8401 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     8461 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     8521 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     8581 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     8641 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     8701 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     8761 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
     8821 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta
     8881 tcgatcacga gactagcctc gagcggccgc ccccttcacc gagggcctat ttcccatgat
     8941 tccttcatat ttgcatatac gatacaaggc tgttagagag ataattggaa ttaatttgac
     9001 tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat ttcttgggta
     9061 gtttgcagtt ttaaaattat gttttaaaat ggactatca
//
